Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,125,423 |
(CG)5→4 |
coding (36‑37/1224 nt) |
glcF ← |
glycolate dehydrogenase, putative iron‑sulfur subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,125,414 | 0 | G | . | 100.0%
| 133.6
/ NA
| 29 | coding (46/1224 nt) | glcF | glycolate dehydrogenase, putative iron‑sulfur subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base . (17/12); total (17/12) |
| * | NC_000913 | 3,125,415 | 0 | C | . | 100.0%
| 133.6
/ NA
| 29 | coding (45/1224 nt) | glcF | glycolate dehydrogenase, putative iron‑sulfur subunit |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base . (17/12); total (17/12) |
GCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACAT > NC_000913/3125334‑3125487
||
gCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAg < 1:544052/81‑1 (MQ=255)
aGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAgcgcg < 2:544053/81‑1 (MQ=255)
aGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAgcgcg > 2:544110/1‑81 (MQ=255)
gAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAgcgcgc < 2:544054/81‑1 (MQ=255)
gAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAgcgcgc > 1:544107/1‑81 (MQ=255)
tGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAgcgcgcgcgtt > 1:544106/1‑79 (MQ=255)
aTAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAgcgcgcgcgttct < 1:544055/81‑5 (MQ=255)
tGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCA‑‑GCGCGCGCGTTCTGCCGCa > 2:544104/1‑81 (MQ=255)
ggCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCA‑‑GCGCGCGCGTTCTGCCGCAt < 1:544056/80‑1 (MQ=255)
aGGTTGCGGTACAAAGTCCGCAGTGAACACAGGCGCGCAGGATGCGGTCGGCTTCCA‑‑GCGCGCGCGTTCTGCCGCATctct < 2:544057/81‑1 (MQ=255)
aGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCA‑‑GCGCGCGCGTTCTGCCGCATctct > 1:544096/1‑81 (MQ=255)
ggTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCA‑‑GCGCGCGCGTTCTGCCGCATCTCtt < 1:544058/81‑1 (MQ=255)
gTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCA‑‑GCGCGCGCGTTCTGCCGCATCTCTTc < 1:544059/81‑1 (MQ=255)
gCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCA‑‑GCGCGCGCGTTCTGCCGCATCTCTTCAGt > 2:544097/1‑81 (MQ=255)
cGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCA‑‑GCGCGCGCGTTCTGCCGCATCTCTTCAGt < 2:544060/80‑1 (MQ=255)
gTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCA‑‑GCGCGCGCGTTCTGCCGCATCTCTTCAGTTaa > 1:544114/1‑81 (MQ=255)
aCAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCA‑‑GCGCGCGCGTTCTGCCGCATCTCTTCAGTTAAtt > 2:544099/1‑81 (MQ=255)
aaaTCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCA‑‑GCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTggg < 2:544061/81‑1 (MQ=255)
aTCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCA‑‑GCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGtt > 1:544109/1‑81 (MQ=255)
tCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCA‑‑GCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGttt < 1:544062/81‑1 (MQ=255)
ccGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCA‑‑GCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTg > 1:544120/1‑81 (MQ=255)
cGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCA‑‑GCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTg < 1:544063/80‑1 (MQ=255)
gCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCA‑‑GCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCa > 1:544116/1‑81 (MQ=255)
cAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCA‑‑GCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCAt < 1:544064/81‑1 (MQ=255)
tGAACACAGGCGCGCAGGATGCTGTCGGCTTCCA‑‑GCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGc < 1:544065/81‑1 (MQ=255)
aaCACAGGCGCGCAGGATGCTGTCGGCTTCCA‑‑GCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCt > 1:544111/1‑81 (MQ=255)
aaCACAGGCGCGCAGGATGCTGTCGGCTTCCA‑‑GCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCt < 1:544066/81‑1 (MQ=255)
gcgcAGGATGCTGTCGGCTTCCA‑‑GCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCaa < 1:544067/81‑1 (MQ=255)
cgcAGGATGCTGTCGGCTTCCA‑‑GCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCa > 1:544102/1‑79 (MQ=255)
gcAGGATGCTGTCGGCTTCCA‑‑GCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAg > 1:544115/1‑81 (MQ=255)
gcAGGATGCTGTCGGCTTCCA‑‑GCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAg > 1:544105/1‑81 (MQ=255)
gcAGGATGCTGTCGGCTTCCA‑‑GCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAg > 2:544101/1‑81 (MQ=255)
cAGGATGCTGTCGGCTTCCA‑‑GCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGt > 2:544112/1‑81 (MQ=255)
tGTCGGCTTCCA‑‑GCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTa > 2:544126/1‑81 (MQ=255)
gTCGGCTTCCA‑‑GCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTa > 1:544108/1‑80 (MQ=255)
cGGCTTCCA‑‑GCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACAt > 2:544113/1‑81 (MQ=255)
||
GCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACAT > NC_000913/3125334‑3125487
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
