Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,134,358 |
(A)7→8 |
coding (228/693 nt) |
yghT → |
putative ATP‑binding protein YghT |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,134,351 | 1 | . | A | 100.0%
| 83.8
/ NA
| 25 | A74E (GCA→GAA) | yghT | putative ATP‑binding protein YghT |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base A (15/10); total (15/10) |
CGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGC‑AAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGT > NC_000913/3134274‑3134424
|
cggtcgTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGC‑aa > 2:545990/1‑80 (MQ=255)
cggtcgTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGC‑aa > 2:545984/1‑80 (MQ=255)
cgTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGC‑aaaaaa > 2:545994/1‑80 (MQ=255)
gTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGC‑aaaaaaaa > 2:546001/1‑80 (MQ=255)
cAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAAATTGCCTGGa > 2:545996/1‑81 (MQ=255)
aaaaTTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAAATTGCCTGGAc < 2:545966/81‑1 (MQ=255)
aaTTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAAATTGCCTGGAc > 2:545989/1‑79 (MQ=255)
aTTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAAATTGCCTGGACCAg > 2:545988/1‑81 (MQ=255)
aCCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAAATTGCCTGGACCAGTTc > 2:546007/1‑81 (MQ=255)
ccGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAAATTGCCTGGACCAGTTcc < 1:545968/81‑1 (MQ=255)
ccGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAAATTGCCTGGACCAGTTcc < 2:545967/81‑1 (MQ=255)
gATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAAATTGCCTGGACCAGTTCCGgcgc > 1:545995/1‑81 (MQ=255)
gAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTa < 2:545969/81‑1 (MQ=255)
tACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCg > 2:546010/1‑81 (MQ=255)
aCATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGt > 1:546005/1‑81 (MQ=255)
aaaaCAATTGAACGAAATCAGGTGAAAACAGCAAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGt > 2:546011/1‑81 (MQ=255)
ttGAACGAAATCAGGTGAAAACAGCAAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGt < 2:545970/81‑1 (MQ=255)
ttGAACGAAATCAGGTGAAAACAGCAAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGt > 1:546002/1‑81 (MQ=255)
gAACGAAATCAGGTGAAAACAGCAAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCg > 2:546014/1‑81 (MQ=255)
gAACGAAATCAGGTGAAAACAGCAAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCg > 2:546017/1‑81 (MQ=255)
aCGAAATCAGGTGAAAACAGCAAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCt < 2:545971/81‑1 (MQ=255)
cGAAATCAGGTGAAAACAGCAAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCtt < 2:545972/81‑1 (MQ=255)
aaTCAGGTGAAAACAGCAAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACt > 1:546024/1‑81 (MQ=255)
aGGTGAAAACAGCAAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTCGTCGCCCGTCGCTTACTgcgc > 2:546023/1‑81 (MQ=255)
ggTGAAAACAGCAAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCt > 1:546019/1‑81 (MQ=255)
gTGAAAACAGCAAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCtt < 2:545973/81‑1 (MQ=255)
tGAAAACAGCAAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCttt > 1:546009/1‑81 (MQ=255)
aaaaCAGCAAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTc < 2:545974/80‑1 (MQ=255)
aaaCAGCAAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGt < 2:545975/81‑1 (MQ=255)
|
CGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGC‑AAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGT > NC_000913/3134274‑3134424
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
