Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,142,898 |
G→A |
A31V (GCA→GTA) |
hybC ← |
hydrogenase 2 large subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,142,898 | 0 | G | A | 100.0%
| 106.5
/ NA
| 31 | A31V (GCA→GTA) | hybC | hydrogenase 2 large subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (12/19); total (12/19) |
CAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCTCAATACGGGTTACCGGATCAATAGTA > NC_000913/3142821‑3142975
|
caATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATACtt < 1:547790/81‑1 (MQ=255)
aaTCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATACttt < 2:547791/81‑1 (MQ=255)
cATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATACTTTCGAAac < 1:547792/80‑1 (MQ=255)
aTGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATACTTTCGAAacga > 1:547838/1‑81 (MQ=255)
cATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATACTTTCGAAACGACGc > 2:547824/1‑81 (MQ=255)
aTCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATACTTTCGAAACGACGc > 2:547828/1‑80 (MQ=255)
cgcgcgGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATACTTTCGAAACGACGCCAt > 1:547825/1‑81 (MQ=255)
cgGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATACTTTCGAAACGACGCCATTTTc < 1:547794/81‑1 (MQ=255)
cgGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATACTTTCGAAACGACGCCATTTTc < 1:547793/81‑1 (MQ=255)
ttCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATACTTTCGAAACGACGCCATTTTCGATTTCGCAATc < 1:547796/80‑1 (MQ=255)
ttCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATACTTTCGAAACGACGCCATTTTCGATTTCGCAATc < 2:547795/80‑1 (MQ=255)
aCGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATACTTTCGAAACGACGCCATTTTCGATTTCGCAATCGAt < 1:547797/80‑1 (MQ=255)
tctcTTCCATGCCGCGCCACATGGTACCGGAAGCCCATACTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGt > 1:547827/1‑80 (MQ=255)
ttCCATGCCGCGCCACATGGTACCGGAAGCCCATACTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCg < 2:547798/75‑1 (MQ=255)
ttCCATGCCGCGCCACATGGTACCGGAAGCCCATACTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTa < 1:547799/77‑1 (MQ=255)
tGCCGCGCCACATGGTACCGGAAGCCCATACTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCt > 1:547829/1‑81 (MQ=255)
gCCGCGCCACATGGTACCGGAAGCCCATACTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCt > 1:547836/1‑80 (MQ=255)
ccGCGCCACATGGTACCGGAAGCCCATACTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCTc > 2:547833/1‑80 (MQ=255)
ccGCGCCACATGGTACCGGAAGCCCATACTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCTc > 1:547831/1‑80 (MQ=255)
ccGCGCCACATGGTACCGGAAGCCCATACTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCTc < 1:547800/80‑1 (MQ=255)
cacaTGGTACCGGAAGCCCATACTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCTCAATACgg < 2:547801/81‑1 (MQ=255)
cacaTGGTACCGGAAGCCCATACTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCTCAATACgg < 1:547802/81‑1 (MQ=255)
caTGGTACCGGAAGCCCATACTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCTCAATACGGGt < 2:547803/81‑1 (MQ=255)
tACCGGAAGCCCATACTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGCAAATTCCCCTCAATACGGGTTACCg > 1:547842/1‑81 (MQ=255)
cGGAAGCCCATACTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCTCAATACGGGTTACCGGAt > 2:547826/1‑81 (MQ=255)
ggAAGCCCATACTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCTCAATACGGGTTACCGGATc < 2:547805/81‑1 (MQ=255)
ggAAGCCCATACTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCTCAATACGGGTTACCGGATc < 1:547804/81‑1 (MQ=255)
gAAGCCCATACTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCTCAATACGGGTTACCGGATc > 2:547830/1‑80 (MQ=255)
ccATACTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCTCAATACGGGTTACCGGATCAATAGt < 1:547807/81‑1 (MQ=255)
cATACTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCTCAATACGGGTTACCGGATCAATAGTa < 1:547809/81‑1 (MQ=255)
cATACTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCTCAATACGGGTTACCGGATCAATAGTa < 2:547808/81‑1 (MQ=255)
|
CAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCTCAATACGGGTTACCGGATCAATAGTA > NC_000913/3142821‑3142975
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
