Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,149,148 |
T→C |
K108K (AAA→AAG) |
yqhA ← |
uncharacterized protein YqhA |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,149,148 | 0 | T | C | 100.0%
| 103.4
/ NA
| 29 | K108K (AAA→AAG) | yqhA | uncharacterized protein YqhA |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (17/12); total (17/12) |
ATCCGGGACATTTTTCGCATCCATAAAGACGCGCAGTAAGTGAATGGAAGAAATTGCCACAATCGACGCTGCTACTTTGTTTTTCAGCGACGTTGCGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGT > NC_000913/3149073‑3149223
|
aTCCGGGACATTTTTCGCATCCATAAAGACGCGCAGTAAGTGAATGGAAGAAATTGCCACAATCGACGCTGCTACCTTGtt > 2:589969/1‑81 (MQ=255)
cGGGACATTTTTCGCATCCATAAAGACGCGCAGTAAGTGAATGGAAGAAATTGCCACAATCGACGCTGCTACCTTGttttt > 2:589980/1‑81 (MQ=255)
cGGGACATTTTTCGCATCCATAAAGACGCGCAGTAAGTGAATGGAAGAAATTGCCACAATCGACGCTGCTACCTTGttttt > 1:589970/1‑81 (MQ=255)
ttCGCATCCATAAAGACGCGCAGTAAGTGAATGGAAGAAATTGCCACAATCGACGCTGCTACCTTGTTTTTCAGCGACGt < 1:589926/80‑1 (MQ=255)
tCGCATCCATAAAGACGCGCAGTAAGTGAATGGAAGAAATTGCCACAATCGACGCTGCTACCTTGTTTTTCAGCGACGtt < 1:589927/80‑1 (MQ=255)
tCGCATCCATAAAGACGCGCAGTAAGTGAATGGAAGAAATTGCCACAATCGACGCTGCTACCTTGTTTTTCAGCGACGtt < 2:589928/80‑1 (MQ=255)
aTCCATAAAGACGCGCAGTAAGTGAATGGAAGAAATTGCCACAATCGACGCTGCTACCTTGTTTTTCAGCGACGTTGCGTc > 2:589975/1‑81 (MQ=255)
tCCATAAAGACGCGCAGTAAGTGAATGGAAGAAATTGCCACAATCGACGCTGCTACCTTGTTTTTCAGCGACGTTGCGTcc < 2:589929/81‑1 (MQ=255)
aTAAAGACGCGCAGTAAGTGAATGGAAGAAATTGCCACAATCGACGTTGCTACCTTGTTTTCCAGCGACGTTGTGTCCAtt > 1:589967/1‑81 (MQ=255)
aaaGACGCGCAGTAAGTGAATGGAAGAAATTGCCACAATCGACGCTGCTACCTTGTTTTTCAGCGACGTTGCGTCCAtttt > 1:589966/1‑81 (MQ=255)
gACGCGCAGTAAGTGAATGGAAGAAATTGCCACAATCGACGCTGCTACCTTGTTTTTCAGCGACGTTGCGTCCATTTTccc > 1:589971/1‑81 (MQ=255)
cgcgCAGTAAGTGAATGGAAGAAATTGCCACAATCGACGCTGCTACCTTGTTTTTCAGCGACGTTGCGTCCATTTTccc > 1:589991/1‑79 (MQ=255)
gcAGTAAGTGAATGGAAGAAATTGCCACAATCGACGCTGCTACCTTGTTTTTCAGCGACGTTGCGTCCATTTTCCccagc > 1:589972/1‑80 (MQ=255)
aGTGAATGGAAGAAATTGCCACAATCGACGCTGCTACCTTGTTTTTCAGCGACGTTGCGTCCATTTTCCCCAGCCAGTTc < 2:589930/80‑1 (MQ=255)
aaTGGAAGAAATTGCCACAATCGACGCTGCTACCTTGTTTTTCAGCGACGTTGCGTCCATTTTCCCCAGCCAGTTCAGCtt < 1:589931/81‑1 (MQ=255)
agaaATTGCCACAATCGACGCTGCTACCTTGTTTTTCAGCGACGTTGCGTCCATTTTCCCCAGCCAGTTCAGCTTCTCttt < 2:589932/81‑1 (MQ=255)
aaaTTGCCACAATCGACGCTGCTACCTTGTTTTTCAGCGACGTTGCGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGt < 1:589933/81‑1 (MQ=255)
aaTTGCCACAATCGACGCTGCTACCTTGTTTTTCAGCGACGTTGCGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGtt > 1:589981/1‑81 (MQ=255)
tGCCACAATCGACGCTGCTACCTTGTTTTTCAGCGACGTTGCGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTtctc > 1:589992/1‑81 (MQ=255)
gCCACAATCGCCGCTGCTACCTTGTTTTTCAGCGACGTTGCGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCg > 2:589993/1‑81 (MQ=255)
gCCACAATCGACGCTGCTACCTTGTTTTTCAGCGACGTTGCGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTtctc < 1:589934/80‑1 (MQ=255)
aaTCGACGCTGCTACCTTGTTTTTCAGCGACGTTGCGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGat < 2:589935/81‑1 (MQ=255)
cGACGCTGCTACCTTGTTTTTCAGCGACGTTGCGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATc > 2:589988/1‑81 (MQ=255)
gACGCTGCTACCTTGTTTTTCAGCGACGTTGCGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATcc < 1:589936/81‑1 (MQ=255)
gctgctACCTTGTTTTTCAGCGACGTTGCGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGc > 1:589977/1‑81 (MQ=255)
gctgctACCTTGTTTTTCAGCGACGTTGCGTCCATTTTCCCCAGCCAGTTCAGCCTCTCTTTGTTCTCGGAGATATCCAGc > 2:589982/1‑81 (MQ=255)
tgctACCTTGTTTTTCAGCGACGTTGCGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCtg > 2:589989/1‑81 (MQ=255)
gctACCTTGTTTTTCAGCGACGTTGCGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCtgt > 2:589976/1‑81 (MQ=255)
ctACCTTGTTTTTCAGCGACGTTGCGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCtg < 1:589937/79‑1 (MQ=255)
|
ATCCGGGACATTTTTCGCATCCATAAAGACGCGCAGTAAGTGAATGGAAGAAATTGCCACAATCGACGCTGCTACTTTGTTTTTCAGCGACGTTGCGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGT > NC_000913/3149073‑3149223
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
