Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,208,695 |
C→T |
S224S (AGC→AGT) |
ttdT → |
L‑tartrate:succinate antiporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,208,695 | 0 | C | T | 100.0%
| 142.4
/ NA
| 41 | S224S (AGC→AGT) | ttdT | L‑tartrate:succinate antiporter |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (18/23); total (18/23) |
GTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGG > NC_000913/3208618‑3208771
|
gttgCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGTGCa > 1:560638/1‑81 (MQ=255)
ttgCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGTGCAt < 1:560589/81‑1 (MQ=255)
ttgCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGTGCAt > 1:560637/1‑81 (MQ=255)
ccGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGTGCAtctc < 1:560590/81‑1 (MQ=255)
gACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGTGCATCTcac < 1:560591/81‑1 (MQ=255)
cGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGTGCATCTcacgcc < 2:560593/79‑1 (MQ=255)
cGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGTGCATCTcacgcc < 1:560592/79‑1 (MQ=255)
gtcaCCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGTGCATCTcacgccacg < 2:560594/78‑1 (MQ=255)
ccATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGTGCATCTCACGCCACGCTGAGTTggg > 1:560648/1‑81 (MQ=255)
cATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGTGCATCTCACGCCACGCTGAGTTgggg > 1:560642/1‑81 (MQ=255)
aTTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGTGCATCTCACGCCACGCTGAGTTGGGGc > 1:560643/1‑81 (MQ=255)
ttttCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGTGCATCTCACGCCACGCTGAGTTGGGGCg < 1:560595/81‑1 (MQ=255)
cTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGTGCATCTCACGCCACGCTGAGTTGGGGCGACTg > 1:560661/1‑81 (MQ=255)
ttGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGTGCATCTCACGCCACGCTGAGTTGGGGCGACTgg > 1:560669/1‑81 (MQ=255)
tGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGTGCATCTCACGCCACGCTGAGTTGGGGCGACTGGt > 1:560653/1‑81 (MQ=255)
gACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGTGCATCTCACGCCACGCTGAGTTGGGGCGACTg < 2:560596/78‑1 (MQ=255)
gACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGTGCATCTCACGCCACGCTGAGTTGGGGCGACTGGtt > 2:560644/1‑81 (MQ=255)
cGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGTGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTcc < 2:560597/81‑1 (MQ=255)
ggCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGTGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCt < 2:560598/81‑1 (MQ=255)
gCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGTGGATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTc < 2:560599/81‑1 (MQ=255)
gATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGTGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCgg < 2:560600/81‑1 (MQ=255)
aTGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGTGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCggg < 2:560601/81‑1 (MQ=255)
gCACCAAACTTGCTGTTAATTGGACTGATGAGAAGTGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATg < 2:560602/81‑1 (MQ=255)
gCACCAAACTTGCTGTTAATTGGACTGATGAAAAGTGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGAt < 1:560603/80‑1 (MQ=255)
gCACCAAACTTGCTGTTAATTGGACTGATGAAAAGTGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATg > 2:560654/1‑81 (MQ=255)
cACCAAACTTGCTGTTAATTGGACTGATGAAAAGTGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGt > 2:560652/1‑81 (MQ=255)
gCTGTTAATTGGACTGATGAAAAGTGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTc < 2:560604/79‑1 (MQ=255)
gCTGTTAATTGGACTGATGAAAAGTGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTc < 1:560605/79‑1 (MQ=255)
gCTGTTAATTGGACTGATGAAAAGTGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAg > 2:560667/1‑81 (MQ=255)
cTGTTAATTGGACTGATGAAAAGTGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAg < 2:560606/80‑1 (MQ=255)
ttAATTGGACTGATGAAAAGTGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCAtt < 2:560607/81‑1 (MQ=255)
aaTTGGACTGATGAAAAGTGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCAttt < 1:560608/80‑1 (MQ=255)
aTTGGACTGATGAAAAGTGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCAtttt < 2:560609/80‑1 (MQ=255)
ttGGACTGATGAAAAGTGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTAc > 1:560671/1‑81 (MQ=255)
tGGACTGATGAAAAGTGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACt > 1:560646/1‑81 (MQ=255)
tgatgaAAAGTGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTc > 1:560679/1‑81 (MQ=255)
tgatgaAAAGTGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTc > 2:560659/1‑81 (MQ=255)
atgaAAAGTGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTc < 2:560610/79‑1 (MQ=255)
gaAAAGTGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTctgct > 1:560655/1‑81 (MQ=255)
aaaGTGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTgg < 2:560611/81‑1 (MQ=255)
aaaGTGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTgg > 2:560672/1‑81 (MQ=255)
|
GTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGG > NC_000913/3208618‑3208771
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
