Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,323,583 |
T→C |
K163R (AAA→AGA) |
glmM ← |
phosphoglucosamine mutase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,323,583 | 0 | T | C | 100.0%
| 153.0
/ NA
| 43 | K163R (AAA→AGA) | glmM | phosphoglucosamine mutase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (19/24); total (19/24) |
ATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGATATAGCGACCCGCGGCATCAACGATACGGCTGGCTTTACCCAGTTCTGCCGAATCAACGC > NC_000913/3323505‑3323655
|
aTGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTCTg < 1:583690/81‑1 (MQ=255)
tgATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTCTGCa > 1:583750/1‑80 (MQ=255)
aTAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTCTGCaaa < 2:583691/80‑1 (MQ=255)
aGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTCTGCAAAACTc < 1:583692/81‑1 (MQ=255)
aGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTCTGCAAAACTc > 1:583740/1‑81 (MQ=255)
gTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTCTGCAAAACTc > 2:583747/1‑80 (MQ=255)
gTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTCTGCAAAACTCg < 2:583693/81‑1 (MQ=255)
tCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTCTGCAAAACTCGa < 1:583694/81‑1 (MQ=255)
gCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTCTGCAAAACTCGatt > 1:583756/1‑80 (MQ=255)
gCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTCTGCAAAACTCGat < 1:583695/80‑1 (MQ=255)
cGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTCTGCAAAACTCGATATAGc > 1:583739/1‑81 (MQ=255)
cGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTCTGCAAAACTCGATATAGc > 2:583744/1‑81 (MQ=255)
tttGCACAATCCGCCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTCTGCAAAACTCGATATAGCGa < 2:583696/81‑1 (MQ=255)
tttGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTCTGCAAAACTCGATATAGCGa > 1:583755/1‑81 (MQ=255)
tGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTCTGCAAAACTCGATATAGCGAcc > 1:583766/1‑81 (MQ=255)
aaTCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTCTGCAAAACTCGATATAGCGACCCGCgg > 2:583763/1‑81 (MQ=255)
aTCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTCTGCAAAACTCGATATAGCGACCCGCGGc < 1:583697/81‑1 (MQ=255)
tCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTCTGCAAAACTCGATATAGCGACCCGCGGCa < 2:583698/81‑1 (MQ=255)
caccacAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTCTGCAAAACTCGATATAGCGACCCGCGGCAt > 1:583749/1‑80 (MQ=255)
ccacAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTCTGCAAAACTCGATATAGCGACCCGCGGCATCaa < 2:583699/81‑1 (MQ=255)
aaTCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTCTGCAAAACTCGATATAGCGACCCGCGGCATCAACGAt < 1:583700/81‑1 (MQ=255)
cAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTCTGCAAAACTCGATATAGCGACCCGCGGCATCAACGATAc < 1:583702/77‑1 (MQ=255)
aaGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTCTGCAAAACTCGATATAGCGACCCGCGGCATCAACGATAc < 1:583701/76‑1 (MQ=255)
aGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTCTGCAAAACTCGATATAGCGACCCGCGGCATCAACGATACggctg > 1:583762/1‑81 (MQ=255)
ttCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTCTGCAAAACTCGATATAGCGACCCGCGGCATCAACGATACggctggc < 2:583703/81‑1 (MQ=255)
tCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTCTGCAAAACTCGATATAGCGACCCGCGGCATCAACGATACggctggct > 2:583748/1‑81 (MQ=255)
aCTGAGGCTAAGTTCGTTCGGGAACGTGGCTCTGCAAAACTCGATATAGCGACCCGCGGCATCAACGATACGGCTGGCttt < 1:583705/81‑1 (MQ=255)
aCTGAGGCTAAGTTCGTTCGGGAACGTGGCTCTGCAAAACTCGATATAGCGACCCGCGGCATCAACGATACGGCTGGCtt < 1:583704/80‑1 (MQ=255)
cTAAGTTCGTTCGGGAACGTGGCTCTGCAAAACTCGATATAGCGACCCGCGGCATCAACGATACGGCTGGCTTTACCCAGt < 2:583706/81‑1 (MQ=255)
aaGTTCGTTCGGGAACGTGGCTCTGCAAAACTCGATATAGCGACCCGCGGCATCAACGATACGGCTGGCTTTACCCAGTTc > 2:583757/1‑81 (MQ=255)
aGTTCGTTCGGGAACGTGGCTCTGCAAAACTCGATATAGCGACCCGCGGCATCAACGATACGGCTGGCTTTACCCAGTTCt < 1:583707/81‑1 (MQ=255)
tcgttcgGGAACGTGGCTCTGCAAAACTCGATATAGCGACCCGCGGCATCAACGATACGGCTGGCTTTACCCAGTTCTGcc > 2:583751/1‑81 (MQ=255)
cgttcgGGAACGTGGCTCTGCAAAACTCGATATAGCGACCCGCGGCATCAACGATACGGCTGGCTTTACCCAGTTCTGcc < 2:583708/80‑1 (MQ=255)
cgttcgGGAACGTGGCTCTGCAAAACTCGATATAGCGACCCGCGGCATCAACGATACGGCTGGCTTTACCCAGTTCTGcc < 2:583709/80‑1 (MQ=255)
gttcgGGAACGTGGCTCTGCAAAACTCGGTATAGCGACCCGCGGCATCAACGATACGGCTGGCTTTACCCAGTTCTGCCg < 1:583710/80‑1 (MQ=255)
gttcgGGAACGTGGCTCTGCAAAACTCGATATAGCGACCCGCGGCATCAACGATACGGCTGGCTTTACCCAGTTCTGCCg > 2:583754/1‑80 (MQ=255)
gggAACGTGGCTCTGCAAAACTCGATATAGCGACCCGTGGCATCAACGATACGGCTGGCTTTACCCAGTTCTGCCGAATc > 1:583771/1‑80 (MQ=255)
gggAACGTGGCTCTGCAAAACTCGATATAGCGACCCGCGGCATCAACGATACGGCTGGCTTTACCCAGTTCTGCCGAATc > 1:583753/1‑80 (MQ=255)
gggAACGTGGCTCTGCAAAACTCGATATAGCGACCCGCGGCATCAACGATACGGCTGGCTTTACCCAGTTCTGCCGAATc < 1:583711/80‑1 (MQ=255)
ggAACGTGGCTCTGCAAAACTCGATATAGCGACCCGCGGCATCAACGATACGGCTGGCTTTACCCAGTTCTGCCGAATCaa < 1:583712/81‑1 (MQ=255)
aCGTGGCTCTGCAAAACTCGATATAGCGACCCGCGGCATCAACGATACGGCTGGCTTTACCCAGTTCTGCCGAATCAACg > 2:583769/1‑80 (MQ=255)
aCGTGGCTCTGCAAAACTCGATATAGCGACCCGCGGCATCAACGATACGGCTGGCTTTACCCAGTTCTGCCGAATCAACGc < 1:583713/81‑1 (MQ=255)
cGTGGCTCTGCAAAACTCGATATAGCGACCCGCGGCATCAACGATACGGCTGGCTTTACCCAGTTCTGCCGAATCAACg > 1:583776/1‑79 (MQ=255)
|
ATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGATATAGCGACCCGCGGCATCAACGATACGGCTGGCTTTACCCAGTTCTGCCGAATCAACGC > NC_000913/3323505‑3323655
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
