Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,332,210 |
C→A |
V176L (GTG→TTG) |
yhbE ← |
inner membrane protein YhbE |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,332,210 | 0 | C | A | 100.0%
| 102.7
/ NA
| 29 | V176L (GTG→TTG) | yhbE | inner membrane protein YhbE |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (20/9); total (20/9) |
GCGCAATTGTACATAAAGTGTACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCACGCCATAACTCACCCAAACCGTCGCCGCACCGACCCCAAAGATAACTCCCCAGGTGTAATCGGTGAGCTTT > NC_000913/3332132‑3332280
|
gcgcAATTGTACATAAAGTGTACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGcgccaagc > 2:630605/1‑81 (MQ=255)
cgcAATTGTACATAAAGTGTACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGcgccaagcc < 1:630567/81‑1 (MQ=255)
cgcAATTGTACATAAAGTGTACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGcgccaagcc < 2:630568/81‑1 (MQ=255)
gcAATTGTACATAAAGTGTACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGcgccaagcca > 2:630596/1‑81 (MQ=255)
aaTTGTACATAAAGTGTACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATa < 2:630569/81‑1 (MQ=255)
aTTGTACATAAAGTGTACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATaa > 1:630598/1‑81 (MQ=255)
aTTGTACATAAAGTGTACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATaa > 1:630622/1‑81 (MQ=255)
aaGTGTACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCaaa > 1:630599/1‑81 (MQ=255)
aGTGTACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAAc > 1:630604/1‑81 (MQ=255)
gtgtACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAAcc < 1:630570/81‑1 (MQ=255)
gtgtACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAAcc > 1:630613/1‑81 (MQ=255)
tACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTc > 1:630610/1‑81 (MQ=255)
tACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTc > 2:630606/1‑81 (MQ=255)
aCAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTcg > 1:630615/1‑81 (MQ=255)
cAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTcg < 2:630571/80‑1 (MQ=255)
aaaaCAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCa < 2:630572/80‑1 (MQ=255)
aaaCAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCAcc < 2:630573/81‑1 (MQ=255)
aaCAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCg < 2:630574/81‑1 (MQ=255)
cAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGAc > 2:630601/1‑81 (MQ=255)
cAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGAc < 1:630575/81‑1 (MQ=255)
gATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGAcccc < 1:630576/81‑1 (MQ=255)
gATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGAccc > 2:630618/1‑80 (MQ=255)
cTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGACCCCa > 1:630608/1‑79 (MQ=255)
gCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGACCCCa > 2:630612/1‑77 (MQ=255)
gTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGACCCCAAAGATaa > 2:630602/1‑81 (MQ=255)
gAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGACCCCAAAGATAACt > 2:630623/1‑81 (MQ=255)
ccGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGACCCCAAAGATAACTCCCCAGgt > 2:630609/1‑81 (MQ=255)
cGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGACCCCAAAGATAACTCCCCAGgtg > 2:630607/1‑81 (MQ=255)
cGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGACCCCAAAGATAACTCCCCAGgtg > 1:630617/1‑81 (MQ=255)
aCGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGACCCCAAAGATAACTCCCCAGgtgt > 1:630614/1‑80 (MQ=255)
tAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGACCCCAAAGATAACTCCCCAGGTGTAATCGGt > 2:630619/1‑81 (MQ=255)
aCCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGACCCCAAAGATAACTCCCCAGGTGTAATCGGTGAGc < 1:630577/81‑1 (MQ=255)
ttttGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGACCCCAAAGATAACTCCCCAGGTGTAATCGGTGAGCttt > 2:630620/1‑81 (MQ=255)
|
GCGCAATTGTACATAAAGTGTACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCACGCCATAACTCACCCAAACCGTCGCCGCACCGACCCCAAAGATAACTCCCCAGGTGTAATCGGTGAGCTTT > NC_000913/3332132‑3332280
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
