Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,332,210 |
C→A |
V176L (GTG→TTG) |
yhbE ← |
inner membrane protein YhbE |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,332,210 | 0 | C | A | 100.0%
| 88.4
/ NA
| 26 | V176L (GTG→TTG) | yhbE | inner membrane protein YhbE |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (13/13); total (13/13) |
CGCAATTGTACATAAAGTGTACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCACGCCATAACTCACCCAAACCGTCGCCGCACCGACCCCAAAGATAACTCCCCAGGTGTAATCGGTGAGCTTTGTAAATA > NC_000913/3332133‑3332287
|
cgcAATTGTACATAAAGTGTACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGcgccaagcc < 2:585509/81‑1 (MQ=255)
cAATTGTACATAAAGTGTACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGcgccaagcca > 2:585538/1‑80 (MQ=255)
aCATAAAGTGTACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCAc > 1:585537/1‑81 (MQ=255)
tAAAGTGTACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCa < 2:585510/81‑1 (MQ=255)
aGTGTACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAAc < 1:585512/81‑1 (MQ=255)
aGTGTACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAAc < 1:585511/81‑1 (MQ=255)
tgtACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCg < 2:585513/81‑1 (MQ=255)
tACAGTAAAAACAGGCTCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTc < 2:585514/81‑1 (MQ=255)
aCAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTcg > 2:585542/1‑81 (MQ=255)
cAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTcg < 1:585515/80‑1 (MQ=255)
aaaCAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCAcc < 2:585516/81‑1 (MQ=255)
aGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGAcc < 2:585517/81‑1 (MQ=255)
gATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGAcccc < 1:585518/81‑1 (MQ=255)
aTCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGAcccc > 2:585545/1‑80 (MQ=255)
cGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGACCCCAAAGa < 1:585519/80‑1 (MQ=255)
ggTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGACCCCAAAGAt > 1:585543/1‑80 (MQ=255)
aGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGACCCCAAAGATAACt < 2:585520/80‑1 (MQ=255)
ggCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGACCCCAAAGATAACTcc > 2:585547/1‑81 (MQ=255)
gCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGACCCCAAAGATAACTccc > 2:585554/1‑81 (MQ=255)
cAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGACCCCAAAGATAACTccc > 1:585553/1‑79 (MQ=255)
ccGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGACCCCAAAGATAACTCCCCAGgt > 2:585551/1‑81 (MQ=255)
gACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGACCCCAAAGATAACTCCCCAGgtg > 1:585550/1‑80 (MQ=255)
cGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGACCCCAAAGATAACTCCCCAGGTGTaa > 2:585560/1‑81 (MQ=255)
aaTAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGACCCCAAAGATAACTCCCCAGGTGTAATCg < 2:585521/81‑1 (MQ=255)
aTAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGACCCCAAAGATAACTCCCCAGGTGTAATCgg < 1:585522/81‑1 (MQ=255)
aaaCCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGACCCCAAAGATAACTCCCCAGGTGTAATCGGt > 1:585559/1‑79 (MQ=255)
aaCCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACAGACCCCAAAGATAACTCCCCAGGTGTAATCGGTGAg > 1:585556/1‑81 (MQ=255)
ttGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGACCCCAAAGATAACTCCCCAGGTGTAATCGGTGAGCTTTg > 2:585546/1‑80 (MQ=255)
ccaagccaTAACTCACCCAAACCGTCGCCGCACCGACCCCAAAGATAACTCCCCAGGTGTAATCGGTGAGCTTTGTAAata > 2:585555/1‑81 (MQ=255)
|
CGCAATTGTACATAAAGTGTACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCACGCCATAACTCACCCAAACCGTCGCCGCACCGACCCCAAAGATAACTCCCCAGGTGTAATCGGTGAGCTTTGTAAATA > NC_000913/3332133‑3332287
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
