Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,368,665 |
T→G |
L280R (CTG→CGG) |
yhcG → |
DUF1016 domain‑containing protein YhcG |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,368,665 | 0 | T | G | 100.0%
| 53.3
/ NA
| 16 | L280R (CTG→CGG) | yhcG | DUF1016 domain‑containing protein YhcG |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (12/4); total (12/4) |
GTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCTGCTAATCGTCGATCTAAAAGTGGGCAAATTCAGCTATAGCGATGCCGGACAGATGAATATGTATCTCAACTACGCC > NC_000913/3368587‑3368741
|
gTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGctgccggc > 2:592976/1‑81 (MQ=255)
gTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGctgccggc < 2:592952/81‑1 (MQ=255)
gTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGctgccggc > 1:592995/1‑81 (MQ=255)
cGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCGGCt > 1:592982/1‑80 (MQ=255)
ggCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCGGCTaa > 1:592985/1‑81 (MQ=255)
ggCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCGGCTa < 2:592953/80‑1 (MQ=255)
cGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCGGCTAAtcgtc > 1:592977/1‑81 (MQ=255)
cGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCGGCTAAtcgtc > 2:592978/1‑81 (MQ=255)
aTTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCGGCTAATCGTCGATCTAAAAGTg > 2:592992/1‑81 (MQ=255)
tGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCGGCTAATCGTCGATCTAAAAGTGGGCAAATTCAGc > 1:592996/1‑81 (MQ=255)
tCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCGGCTAATCGTCGATCTAAAAGTGGGCAAATTCAGCTATAg > 1:592983/1‑81 (MQ=255)
gtgtCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCGGCTAATCGTCGATCTAAAAGTGGGCAAATTCAGCTATAGc > 1:592994/1‑80 (MQ=255)
gtCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCGGCTAATCGTCGATCTAAAAGTGGGCAAATTCAGCTATAGCGAt > 1:592991/1‑81 (MQ=255)
cGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCGGCTAATCGTCGATCTAAAAGTGGGCAAATTCAGCTATAGCGATGc > 1:592987/1‑81 (MQ=255)
ctgTTTTTCCACCGCCGTTTACGCTGCCGGCTAATCGTCGATCTAAAAGTGGGCAAATTCAGCTATAGCGATGCCGGAc < 2:592954/79‑1 (MQ=255)
tCCACCGCCGTTTACGCTGCCGGCTAATCGTCGATCTAAAAGTGGGCAAATTCAGCTATAGCGATGCCGGACAGATGAat < 2:592955/80‑1 (MQ=255)
cACCGCCGTTTACGCTGCCGGCTAATCGTCGATCTAAAAGTGGGCAAATTCAGCTATAGCGATGCCGGACAGATGAATATg < 2:592956/81‑1 (MQ=255)
gTTTACGCTGCCGGCTAATCGTCGATCTAAAAGTGGGCAAATTCAGCTATAGCGATGCCGGACAGATGAATATGTAtctc > 2:593005/1‑80 (MQ=255)
gCTGCCGGCTAATCGTCGATCTAAAAGTGGGCAAATTCAGCTATAGCGATGCCGGACAGATGAATATGTATCTCAACTACg > 2:593007/1‑81 (MQ=255)
gccggcTAATCGTCGATCTAAAAGTGGGCAAATTCAGCTATAGCGATGCCGGACAGATGAATATGTATCTCAACTACGcc > 2:592997/1‑80 (MQ=255)
|
GTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCTGCTAATCGTCGATCTAAAAGTGGGCAAATTCAGCTATAGCGATGCCGGACAGATGAATATGTATCTCAACTACGCC > NC_000913/3368587‑3368741
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
