Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,432,299 |
+C |
coding (166/474 nt) |
smg ← |
DUF494 domain‑containing protein Smg |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,432,296 | 1 | . | C | 100.0%
| 68.8
/ NA
| 20 | L57V (TTG→GTG) | smg | DUF494 domain‑containing protein Smg |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (8/12); total (8/12) |
GCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAA‑CCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATATCTTCTCGCTCAAAACCT > NC_000913/3432217‑3432366
|
gCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAA‑c < 2:605733/81‑1 (MQ=255)
aTCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAA‑ccc < 1:605734/81‑1 (MQ=255)
tCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAA‑cccc > 1:605768/1‑80 (MQ=255)
aGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAA‑ccccttc < 1:605735/81‑5 (MQ=255)
tCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCCTTCCt < 2:605736/81‑1 (MQ=255)
acTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCCTTCCTGATAATCa < 1:605737/81‑1 (MQ=255)
tctTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCCTTCCTGATAATCAg > 2:605766/1‑80 (MQ=255)
ttCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCCTTCCTGATAATCAGc < 1:605738/79‑1 (MQ=255)
ccGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCCTTCCTGATAATCAGCa < 2:605740/78‑1 (MQ=255)
ccGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCCTTCCTGATAATCAGCAAg < 2:605739/80‑1 (MQ=255)
cGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCCTTCCTGATAATCAGCAAGt < 2:605741/80‑1 (MQ=255)
aaTACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCCTTCCTGATAATCAGCAAGTTTTTCCAGc > 2:605764/1‑80 (MQ=255)
aCGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCCTTCCTGATAATCAGCAAGTTTTTCCAGCCa > 1:605771/1‑79 (MQ=255)
cGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAg < 2:605742/81‑1 (MQ=255)
gagGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTg > 2:605778/1‑81 (MQ=255)
agGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGt < 1:605743/81‑1 (MQ=255)
gATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTg > 2:605783/1‑78 (MQ=255)
tCCGAGGCCAGTTGCATCGGTTCTGCCAACCCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGa > 1:605772/1‑80 (MQ=255)
tCCGAGGCCAGTTGCATCGGTTCTGCCAACCCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGa > 1:605765/1‑80 (MQ=255)
gCCAGTTGCATCGGTTCTGCCAACCCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATAtcttc < 1:605744/81‑1 (MQ=255)
cATCGGTTCTGCCAACCCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATATCTTCTCGCTCa < 2:605745/80‑1 (MQ=255)
cGGTTCTGCCAACCCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATATCTTCTCGCTCAAAAc > 2:605775/1‑81 (MQ=255)
ggTTCTGCCAACCCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATATCTTCTCGCTCAAAAc < 2:605746/80‑1 (MQ=255)
gTTCTGCCAACCCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATATCTTCTCGCTCAAAACCt < 2:605747/81‑1 (MQ=255)
|
GCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAA‑CCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATATCTTCTCGCTCAAAACCT > NC_000913/3432217‑3432366
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
