Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,474,329 |
T→G |
Q75P (CAG→CCG) |
rpsL ← |
30S ribosomal subunit protein S12 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,474,329 | 0 | T | G | 97.4%
| 130.2
/ ‑4.0
| 38 | Q75P (CAG→CCG) | rpsL | 30S ribosomal subunit protein S12 |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base G (18/19); total (18/20) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01 |
ACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACG > NC_000913/3474253‑3474405
|
accacGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCGGCAg < 2:614017/81‑1 (MQ=255)
gtgGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCGGCAGGTTGTGACCtt > 2:614064/1‑80 (MQ=255)
tgGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCGGCAGGTTGTGACCTTc < 2:614018/80‑1 (MQ=255)
ggTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCGGCAGGTTGTGACCTTcac > 2:614066/1‑81 (MQ=255)
gTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCGGCAGGTTGTGACCTTcac < 2:614019/80‑1 (MQ=255)
aCGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCGGCAGGTTGTGACCTTCaccacc < 2:614020/81‑1 (MQ=255)
cGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCGGCAGGTTGTGACCTTCaccacc > 2:614061/1‑80 (MQ=255)
gAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCGGCAGGTTGTGACCTTCACCACCGa > 1:614051/1‑81 (MQ=255)
gAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCGGCAGGTTGTGACCTTCACCACCGa > 1:614077/1‑81 (MQ=255)
aaCACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCGGCAGGTTGTGACCTTCACCACCGAt > 1:614093/1‑81 (MQ=255)
cacCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGt < 2:614021/81‑1 (MQ=255)
cacCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCGGCAGGTTGTGACCTTCACCACCGATg > 1:614074/1‑80 (MQ=255)
ggAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCGGCAGGTTGTGACCTTCACCACCGATGTAGGAAg < 1:614022/81‑1 (MQ=255)
gAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCGGCAGGTTGTGACCTTCACCACCGATGTAGGAAGt < 1:614023/81‑1 (MQ=255)
gAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCGGCAGGTTGTGACCTTCACCACCGATGTAGGAAGt > 1:614069/1‑81 (MQ=255)
aGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCGGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTc < 2:614024/81‑1 (MQ=255)
gTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCGGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCAc > 1:614079/1‑81 (MQ=255)
tCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCGGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACt > 2:614094/1‑81 (MQ=255)
ttAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCGGCAGGT‑‑TGACCTTCACCACCGATGTAGGAAGTCACTTCGa > 1:614072/1‑80 (MQ=255)
tAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCGGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCg > 1:614075/1‑80 (MQ=255)
tAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCGGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCg > 1:614073/1‑80 (MQ=255)
acGACCGCCACGGATCAGGATCACGGAGTGCTCCGGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAAcc < 2:614025/81‑1 (MQ=255)
aCCGCCACGGATCAGGATCACGGAGTGCTCCGGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGtt < 1:614026/81‑1 (MQ=255)
cGCCACGGATCAGGATCACGGAGTGCTCCGGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAg > 1:614080/1‑81 (MQ=255)
ccACGGATCAGGATCACGGAGTGCTCCGGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTc < 2:614027/81‑1 (MQ=255)
aCGGATCAGGATCACGGAGTGCTCCGGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAg < 1:614028/81‑1 (MQ=255)
aCGGATCAGGATCACGGAGTGCTCCGGCAGGATGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAg < 2:614029/81‑1 (MQ=255)
ggatcaggatcaCGGAGTGCTCCGGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGAc > 1:614092/1‑81 (MQ=255)
ggatcaggatcaCGGAGTGCTCCGGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGAc < 1:614030/81‑1 (MQ=255)
aggatcaCGGAGTGCTCCGGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAaca < 2:614031/81‑1 (MQ=255)
aCGGAGTGCTCCGGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCa < 2:614033/80‑1 (MQ=255)
aCGGAGTGCTCCGGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCAt < 2:614032/81‑1 (MQ=255)
ggAGTGCTCCGGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATAc > 1:614081/1‑81 (MQ=255)
gAGTGCTCCGGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACt < 2:614036/81‑1 (MQ=255)
gAGTGCTCCGGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACt < 2:614034/81‑1 (MQ=255)
gAGTGCTCCGGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACt < 1:614035/81‑1 (MQ=255)
tGCTCCGGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTa > 2:614097/1‑81 (MQ=255)
tCCGGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACg > 2:614102/1‑80 (MQ=255)
|
ACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACG > NC_000913/3474253‑3474405
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
