Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,489,824 |
T→A |
A119A (GCA→GCT) |
argD ← |
N‑acetylornithine aminotransferase/N‑succinyldiaminopimelate aminotransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,489,824 | 0 | T | A | 100.0%
| 141.0
/ NA
| 40 | A119A (GCA→GCT) | argD | N‑acetylornithine aminotransferase/N‑succinyldiaminopimelate aminotransferase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (23/17); total (23/17) |
CGAGCGACCATGAAAAGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGTGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAACACCACGCGTTCGGCAAACGTTGCCT > NC_000913/3489746‑3489897
|
cgagcgaCCATGAAAAGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGAGc < 2:666415/81‑1 (MQ=255)
gagcgaCCATGAAAAGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGAGc > 2:666458/1‑80 (MQ=255)
cgaCCATGAAAAGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGAGCCAGt < 2:666416/81‑1 (MQ=255)
cgaCCATGAAAAGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGAGCCAGt > 1:666472/1‑81 (MQ=255)
cATGAAAAGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGAGCCAGTTTa > 2:666479/1‑80 (MQ=255)
aaGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGAGCCAGTTTAAAGGCgg > 1:666475/1‑81 (MQ=255)
aaGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGAGCCAGTTTAAAGGCgg > 2:666453/1‑81 (MQ=255)
aGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGAGCCAGTTTAAAGGCGGt > 1:666466/1‑81 (MQ=255)
aGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGAGCCAGTTTAAAGGCGGt < 1:666417/81‑1 (MQ=255)
cGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGAGCCAGTTTAAAGGCGGttt < 2:666418/81‑1 (MQ=255)
gTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGAGCCAGTTTAAAGGCGGTTTc > 1:666467/1‑81 (MQ=255)
gTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGAGCCAGTTTAAAGGCGGTTTc > 2:666494/1‑81 (MQ=255)
tATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGAGCCAGTTTAAAGGCGGTTTCGt > 1:666460/1‑81 (MQ=255)
aTGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGAGCCAGTTTAAAGGCGGTTTCGt < 2:666419/80‑1 (MQ=255)
tGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGAGCCAGTTTAAAGGCGGTTTCGtt < 2:666420/80‑1 (MQ=255)
aaGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGAGCCAGTTTAAAGGCGGTTTCGTTAGCt > 2:666457/1‑81 (MQ=255)
ggCAATAATCTTTGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGAGCCAGTTTAAAGGCGGTTTCGTTAGCTTc < 2:666421/81‑1 (MQ=255)
tGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGTGAGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTTCCGGAGt > 1:666482/1‑81 (MQ=255)
gTTTTGAACGGGCTATGACGCACACAGGCGTGATGGCGAGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTc < 2:666422/81‑1 (MQ=255)
gTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGAGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGtt < 1:666423/80‑1 (MQ=255)
gTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGAGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGtt > 1:666480/1‑80 (MQ=255)
ttGAACGGGCTATGACGCACACAGGCGTAATGGCGAGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCAt < 1:666424/80‑1 (MQ=255)
tGAACGGGCTATGACGCACACAGGCGTAATGGCGAGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATa > 2:666477/1‑80 (MQ=255)
gAACGGGCTATGACGCACACAGGCGTAATGGCGAGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATaaa > 1:666487/1‑81 (MQ=255)
cGGGCTATGACGCACACAGGCGTAATGGCGAGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAacac > 1:666471/1‑81 (MQ=255)
gggCTATGACGCACACAGGCGTAATGGCGAGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAacac > 1:666463/1‑80 (MQ=255)
gggCTATGACGCACACAGGCGTAATGGCGAGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAAcacc < 2:666425/81‑1 (MQ=255)
ggCTATGACGCACACAGGCGTAATGGCGAGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAAcacca > 2:666492/1‑81 (MQ=255)
gCTATGACGCACACAGGCGTAATGGCGAGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAAcaccac < 1:666426/81‑1 (MQ=255)
cTATGACGCACACAGGCGTAATGGCGAGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAACACCAcg > 1:666464/1‑81 (MQ=255)
aCGCACACAGGCGTAATGGCGAGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAACACCACGCGtt < 2:666427/80‑1 (MQ=255)
aCGCACACAGGCGTAATGGCGAGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAACACCACGCGTTc < 1:666428/81‑1 (MQ=255)
cGCACACAGGCGTAATGGCGAGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAACACCACGCGTTCg > 2:666468/1‑81 (MQ=255)
acaGGCGTAATGGCGAGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAACACCACGCGTTCGGCaaa > 2:666491/1‑81 (MQ=255)
gCGTAATGGCGAGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAATCACCACGCGTTCGGCAAACGtt < 1:666429/81‑1 (MQ=255)
gCGTAATGGCGAGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAACACCACGCGTTCGGCAAACGtt > 1:666470/1‑81 (MQ=255)
gCGTAATGGCGAGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAACACCACGCGTTCGGCAAACGtt < 1:666430/81‑1 (MQ=255)
cGTAATGGCGAGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAACACCACGCGTTCGGCAAACGTTg > 1:666476/1‑81 (MQ=255)
aTGGCGAGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAACACCACGCGTTCGGCAAACGTTGCCt < 2:666431/80‑1 (MQ=255)
aTGGCGAGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAACACCACGCGTTCGGCAAACGTTGCCt > 1:666489/1‑80 (MQ=255)
|
CGAGCGACCATGAAAAGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGTGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAACACCACGCGTTCGGCAAACGTTGCCT > NC_000913/3489746‑3489897
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
