Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,549,669 |
C→T |
P134P (CCG→CCA) |
malQ ← |
4‑alpha‑glucanotransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,549,669 | 0 | C | T | 100.0%
| 141.3
/ NA
| 40 | P134P (CCG→CCA) | malQ | 4‑alpha‑glucanotransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (23/17); total (23/17) |
CCCAGTTTTTTTCCGATCGCAGCGTATAAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGCGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGGTGAGTGTCAGCGTGT > NC_000913/3549593‑3549745
|
cccAGTTTTTTTCCGATCGCAGCGTATAAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGTGGtt > 1:630603/1‑81 (MQ=255)
cccAGTTTTTTTCCGATCGCAGCGTATAAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGTGGtt < 2:630571/81‑1 (MQ=255)
gTTTTTTTCCGATCGCAGCGTATAAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGTGGTTCGTa < 2:630572/81‑1 (MQ=255)
ttttttCCGATCGCAGCGTATAAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGTGGTTCGTaa > 2:630616/1‑80 (MQ=255)
ttttttCCGATCGCAGCGTATAAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGTGGTTCGTaa > 2:630601/1‑80 (MQ=255)
tttttCCGATCGCAGCGTATAAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGTGGTTCGTaa > 1:630619/1‑79 (MQ=255)
cGATCGCAGCGTATAAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGTGGTTCGTAACAGCGtt < 2:630573/80‑1 (MQ=255)
gATCGCAGCGTATAAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGTGGTTCGTAACAGCGTTTc > 2:630604/1‑81 (MQ=255)
gATCGCAGCGTATAAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGTGGTTCGTAACAGCGTTTc > 2:630599/1‑81 (MQ=255)
gCAGCGTATAAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGTGGTTCGTAACAGCGTTTCgggg > 2:630637/1‑81 (MQ=255)
gCGTATAAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGTGGTTCGTAACAGCGTTTCGGGGCGa < 1:630574/81‑1 (MQ=255)
tataAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGTGGTTCGTAACAGCGTTTCGGGGCGACaa > 2:630620/1‑81 (MQ=255)
tataAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGTGGTTCGTAACAGCGTTTCGGGGCGACaa > 1:630622/1‑81 (MQ=255)
ataAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGTGGTTCGTAACAGCGTTTCGGGGCGACAAt > 1:630611/1‑81 (MQ=255)
aaGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGTGGTTCGTAACAGCGTTTCGGGGCGACAATCAc > 1:786567/1‑81 (MQ=255)
cTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGTGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCg < 1:630575/81‑1 (MQ=255)
gAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGTGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCgg > 1:630608/1‑80 (MQ=255)
aaCGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGTGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCgg < 2:630576/79‑1 (MQ=255)
cGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGTGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAAt < 2:630577/81‑1 (MQ=255)
gCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGTGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATg > 1:630657/1‑81 (MQ=255)
cAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGTGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATgc < 2:630578/81‑1 (MQ=255)
aGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGTGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATgcg > 1:630614/1‑81 (MQ=255)
aCCCCACAGCTTTTGTTTATTCAGCAACGCCTGTGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATgcgcgcg > 2:630613/1‑81 (MQ=255)
ccccACAGCTTTTGTTTATTCAGCAACGCCTGTGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATgcgcgcgc > 2:630598/1‑81 (MQ=255)
aGCTTTTGTTTATTCAGCAACGCCTGTGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATGCGCGCGCTGgtcg < 1:630579/81‑1 (MQ=255)
aGCTTTTGTTTATTCAGCAACGCCTGTGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATGCGCGCGCTGgtcg < 1:630580/81‑1 (MQ=255)
gCTTTTGTTTATTCAGCAACGCCTGTGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATGCGCGCGCTGgtcgt < 1:630581/81‑1 (MQ=255)
gCTTTTGTTTATTCAGCAACGCCTGTGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATGCGCGCGCTGgtcgt > 2:630609/1‑81 (MQ=255)
tGTTTATTCAGCAACGCCTGTGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTg < 2:630582/80‑1 (MQ=255)
gTTTATTCAGCAACGCCTGTGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTggg > 1:630629/1‑81 (MQ=255)
tttATTCAGCAACGCCTGTGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGGt > 1:691048/1‑81 (MQ=255)
tttATTCAGCAACGCCTGTGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGGt < 2:630583/81‑1 (MQ=255)
tATTCAGCAACGCCTGTGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGGTg < 1:630584/80‑1 (MQ=255)
ttCAGCAACGCCTGTGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGGTGAgt < 1:630585/81‑1 (MQ=255)
cAGCAACGCCTGTGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGGTGAgtgt > 2:630621/1‑81 (MQ=255)
gCAACGCCTGTGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGGTGAGTGTCa < 2:630586/81‑1 (MQ=255)
aaCGCCTGTGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGGTGAGTGTCAGc > 1:630625/1‑81 (MQ=255)
cGCCTGTGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGGTGAGTGTCAGCgt > 1:630617/1‑81 (MQ=255)
gCCTGTGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGGTGAGTGTCAGCgtg > 2:630615/1‑81 (MQ=255)
ccTGTGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGGTGAGTGTCAGCgtgt < 2:739882/81‑1 (MQ=255)
|
CCCAGTTTTTTTCCGATCGCAGCGTATAAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGCGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGGTGAGTGTCAGCGTGT > NC_000913/3549593‑3549745
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
