Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,549,669 |
C→T |
P134P (CCG→CCA) |
malQ ← |
4‑alpha‑glucanotransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,549,669 | 0 | C | T | 100.0%
| 117.0
/ NA
| 32 | P134P (CCG→CCA) | malQ | 4‑alpha‑glucanotransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (19/13); total (19/13) |
CCCCAGTTTTTTTCCGATCGCAGCGTATAAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGCGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGGTGAGTGTCAGCGTGTG > NC_000913/3549592‑3549746
|
ccccAGTTTTTTTCCGATCGCAGCGTATAAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGTGGt > 2:681095/1‑81 (MQ=255)
ccccAGTTTTTTTCCGATCGCAGCGTATAAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGTGGt > 2:681092/1‑81 (MQ=255)
cccAGTTTTTTTCCGATCGCAGCGTATAAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGTGGtt > 1:681107/1‑81 (MQ=255)
tttttttCCGATCGCAGCGTATAAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGTGGTTCGTaa > 2:681116/1‑81 (MQ=255)
tttttCCGATCGCAGCGTATAAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGTGGTTCGTAAc < 1:681055/80‑1 (MQ=255)
ttttCCGATCGCAGCGTATAAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGTGGTTCGTAACAg > 1:681104/1‑81 (MQ=255)
ttCCGATCGCAGCGTATAAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGTGGTTCGTAACAGCg > 1:681086/1‑81 (MQ=255)
ttCCGATCGCAGCGTATAAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGTGGTTCGTAACAGCg > 1:681087/1‑81 (MQ=255)
tCCGATCGCAGCGTATAAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGTGGTTCGTAACAGCGt > 1:681094/1‑81 (MQ=255)
cGATCGCAGCGTATAAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATACAGCAACGCCTGTGGTTCGTAACAGCGtt < 2:681056/80‑1 (MQ=255)
aTCGCAGCGTATAAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGTGGTTCGTAACAGCGTTTCg > 1:681098/1‑81 (MQ=255)
cAGCGTATAAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGTGGTTCGTAACAGCGTTTCGGGGc > 1:681109/1‑81 (MQ=255)
aGCGTATAAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGTGGTTCGTAACAGCGTTTCGGGGc > 2:681101/1‑80 (MQ=255)
aGCGTATAAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGTGGTTCGTAACAGCGTTTCGGGGCg < 1:681057/81‑1 (MQ=255)
cGTATAAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGTGGTTCGTAACAGCGTTTCGGGGCGAc < 1:681058/81‑1 (MQ=255)
gTATAAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGTGGTTCGTAACAGCGTTTCGGGGCGAc > 1:681108/1‑80 (MQ=255)
ataAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGTGGTTCGTAACAGCGTTTCGGGGCGACAAt > 1:681078/1‑81 (MQ=255)
aaCGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGTGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGc < 1:681059/80‑1 (MQ=255)
aaCGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGTGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGc > 2:681102/1‑80 (MQ=255)
ggCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGTGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATgcgc < 1:681060/81‑1 (MQ=255)
cACCCCACAGCTTTTGTTTATTCAGCAACGCCTGTGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATgcgcgc > 1:681123/1‑81 (MQ=255)
cccACAGCTTTTGTTTATTCAGCAACGCCTGTGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATGCGCGCGCt < 2:681061/81‑1 (MQ=255)
aGCTTTTGTTTATTCAGCAACGCCTGTGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATGCGCGCGCTGgtcg > 2:681110/1‑81 (MQ=255)
gCTTTTGTTTATTCAGCAACGCCTGTGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATGCGCGCGCTGgtcgt < 1:681062/81‑1 (MQ=255)
ttGTTTATTCAGCAACGCCTGTGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATGCGCGCGCTGGTCGTcc < 2:681063/79‑1 (MQ=255)
ttCAGCAACGCCTGTGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGGTGAgt > 2:681111/1‑81 (MQ=255)
ttCAGCAACGCCTGTGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGGTGAg < 1:681064/80‑1 (MQ=255)
gCAACGCCTGTGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGGTGAgtgt > 1:681115/1‑79 (MQ=255)
gCCTGTGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGGTGAGTGTCAGCgtg < 2:681065/81‑1 (MQ=255)
ccTGTGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGGTGAGTGTCAGCgtgt > 1:681121/1‑81 (MQ=255)
cTGTGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGGTGAGTGTCAGCGtgtg < 2:681066/81‑1 (MQ=255)
cTGTGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGGTGAGTGTCAGCGtgtg < 2:681067/81‑1 (MQ=255)
|
CCCCAGTTTTTTTCCGATCGCAGCGTATAAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGCGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGGTGAGTGTCAGCGTGTG > NC_000913/3549592‑3549746
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
