Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,508,746 |
G→A |
intergenic (+4/+88) |
rlhA → / ← yncJ |
23S rRNA 5‑hydroxycytidine C2501 synthase/protein YncJ |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,508,746 | 0 | G | A | 100.0%
| 115.6
/ NA
| 32 | intergenic (+4/+88) | rlhA/yncJ | 23S rRNA 5‑hydroxycytidine C2501 synthase/protein YncJ |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (17/15); total (17/15) |
GTTACCGGGAAGCGTAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCG > NC_000913/1508670‑1508821
|
gTTACCGGGAAGCGTAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACACCAg > 2:286243/1‑81 (MQ=255)
ttACCGGGAAGCGTAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACACCAGt > 2:286244/1‑81 (MQ=255)
ttACCGGGAAGCGTAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACACCAGt < 1:286199/81‑1 (MQ=255)
ccGGGAAGCGTAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACACCAGtttt > 1:286226/1‑81 (MQ=255)
ccGGGAAGCGTAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACACCAGttt < 1:286201/80‑1 (MQ=255)
ccGGGAAGCGTAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACACCAGttt < 2:286200/80‑1 (MQ=255)
cGGGAAGCGTAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACACCAGTTTTc < 2:286202/81‑1 (MQ=255)
gggAAGCGTAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACACCAGTTTTCt > 1:286231/1‑81 (MQ=255)
ggAAGCGTAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACACCAGTTTTCTg > 1:286242/1‑81 (MQ=255)
gAAGCGTAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACACCAGTTTTCTgg > 2:286230/1‑81 (MQ=255)
aGCGTAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACACCAGTTTTCTGGtt < 2:286203/81‑1 (MQ=255)
tAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACACCAGTTTTCTGGTTACTc < 1:286204/81‑1 (MQ=255)
aGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACACCAGTTTTCTGGTTACTca > 1:286228/1‑81 (MQ=255)
aGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACACCAGTTTTCTGGTTACTca > 1:286236/1‑81 (MQ=255)
gTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACACCAGTTTTCTGGTTACTcac < 1:286205/81‑1 (MQ=255)
tGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACACCAGTTTTCTGGTTACTcaca < 2:286206/81‑1 (MQ=255)
aTCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACACCAGTTTTCTGGTTACTCACAACt > 1:286234/1‑80 (MQ=255)
cGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACACCAGTTTTCTGGTTACTCACAACTTAt < 1:286207/80‑1 (MQ=255)
gTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACACCAGTTTTCTGGTTACTCACAACTTATTg < 1:286208/81‑1 (MQ=255)
aTGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACACCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCAt > 2:286237/1‑80 (MQ=255)
ctgAAAACATTGCCGAAGCGAAAAGGGTAAAACACCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATtg < 1:286209/79‑1 (MQ=255)
ctgAAAACATTGCCGAAGCGAAAAGGGTAAAACACCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATtg < 2:286210/79‑1 (MQ=255)
gAAAACATTGCCGAAGCGAAAAGGGTAAAACACCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATtgcctg > 1:286256/1‑81 (MQ=255)
aaaaCATTGCCGAAGCGAAAAGGGTAAAACACCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATtgcctgc < 2:286211/81‑1 (MQ=255)
aTTGCCGAAGCGAAAAGGGTAAAACACCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCggg > 1:286240/1‑80 (MQ=255)
cGAAGCGAAAAGGGTAAAACACCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGc < 1:286212/81‑1 (MQ=255)
gAAGCGAAAAGGGTAAAACACCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCg < 1:286213/81‑1 (MQ=255)
cGAAAAGGGTAAAACACCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTAc > 2:286235/1‑81 (MQ=255)
gAAAAGGGTAAAACACCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTAcg > 1:286257/1‑81 (MQ=255)
aaaGGGTAAAACACCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTAcgcc > 1:286249/1‑81 (MQ=255)
ggTAAAACACCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCAt > 1:286239/1‑81 (MQ=255)
aaaaCACCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCg > 2:286252/1‑81 (MQ=255)
|
GTTACCGGGAAGCGTAGTGGCATCCGTAAGTCCGGATGAGCTGCTGAAAACATTGCCGAAGCGAAAAGGGTAAAACGCCAGTTTTCTGGTTACTCACAACTTATTGAATCTGCATGATATTGCCTGCCGGGTAAGGCGTTACGCCGCATCCG > NC_000913/1508670‑1508821
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
