Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,625,496 |
A→G |
F629L (TTT→CTT) |
dcp ← |
peptidyl‑dipeptidase Dcp |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,625,496 | 0 | A | G | 100.0%
| 108.2
/ NA
| 30 | F629L (TTT→CTT) | dcp | peptidyl‑dipeptidase Dcp |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (12/18); total (12/18) |
TTACCTCTGGAAAGGATCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTTAATCCGCCCTGCTCAACAAACCACTGATAACCATCATCGGCCAACATTTGCGTCCACAGATAAGCGTAATAACCTGCAGCATATCC > NC_000913/1625426‑1625562
|
ttACCTCTGGAAAGGATCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTTAATCCGCCCTGCTCAACAAGCCACTGATaa > 2:304173/1‑81 (MQ=255)
tGGAAAGGATCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTTAATCCGCCCTGCTCAACAAGCCACTGATAACcatc < 2:304142/79‑1 (MQ=255)
gAAAGGATCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTTAATCCGCCCTGCTCAACAAGCCACTGATAACCatcatc < 1:304143/80‑1 (MQ=255)
ggATCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTTAATCCGCCCTGCTCAACAAGCCACTGATAACCATCATCGGCCa < 1:304144/81‑1 (MQ=255)
gATCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTTAATCCGCCCTGCTCAACAAGCCACTGATAACCATCATCGGCCaa > 1:304180/1‑81 (MQ=255)
tCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTTAATCCGCCCTGCTCAACAAGCCACTGATAACCATCATCGGCCAACa < 2:304145/81‑1 (MQ=255)
ccTCGCGAAAACGCAGCCCATTTTCACGCGTTAATCCGCCCTGCTCAACAAGCCACTGATAACCATCATCGGCCAACAttt < 1:304147/81‑1 (MQ=255)
ccTCGCGAAAACGCAGCCCATTTTCACGCGTTAATCCGCCCTGCTCAACAAGCCACTGATAACCATCATCGGCCAACAttt < 2:304146/81‑1 (MQ=255)
cTCGCGAAAACGCAGCCCATTTTCACGCGTTAATCCGCCCTGCTCAACAAGCCACTGATAACCATCATCGGCCAACATTTg > 1:304185/1‑81 (MQ=255)
gcgAAAACGCAGCCCATTTTCACGCGTTAATCCGCCCTGCTCAACAAGCCACTGATAACCATCATCGGCCAACATTTGc < 2:304149/79‑1 (MQ=255)
gcgAAAACGCAGCCCATTTTCACGCGTTAATCCGCCCTGCTCAACAAGCCACTGATAACCATCATCGGCCAACATTTGc < 2:304148/79‑1 (MQ=255)
gAAAACGCAGCCCATTTTCACGCGTTAATCCGCCCTGCTCAACAAGCCACTGATAACCATCATCGGCCAACATTTGCGTc > 2:304192/1‑80 (MQ=255)
aaaaCGCAGCCCATTTTCACGCGTTAATCCGCCCTGCTCAACAAGCCACTGATAACCATCATCGGCCAACATTTGCGTCca > 2:304190/1‑81 (MQ=255)
aaaaCGCAGCCCATTTTCACGCGTTAATCCGCCCTGCTCAACAAGCCACTGATAACCATCATCGGCCAACATTTGCGTCca < 2:304150/81‑1 (MQ=255)
aaaCGCAGCCCATTTTCACGCGTTAATCCGCCCTGCTCAACAAGCCACTGATAACCATCATCGGCCAACATTTGCGTCca > 2:304194/1‑80 (MQ=255)
aaCGCAGCCCATTTTCACGCGTTAATCCGCCCTGCTCAACAAGCCACTGATAACCATCATCGGCCAACATTTGCGTCca > 1:304188/1‑79 (MQ=255)
aCGCAGCCCATTTTCACGCGTTAATCCGCCCTGCTCAACAAGCCACTGATAACCATCATCGGCCAACATTTGCGTCCACAg < 2:304151/81‑1 (MQ=255)
gCCCATTTTCACGCGTTAATCCGCCCTGCTCAACAAGCCACTGATAACCATCATCGGCCAACATTTGCGTCCACAGATAAg < 1:304152/81‑1 (MQ=255)
gCCCATTTTCACGCGTTAATCCGCCCTGCTCAACAAGCCACTGATAACCATCATCGGCCAACATTTGCGTCCACAGATAAg > 1:304184/1‑81 (MQ=255)
cATTTTCACGCGTTAATCCGCCCTGCTCAACAAGCCACTGATAACCATCATCGGCCAACATTTGCGTCCACAGATAAGCg > 1:304186/1‑80 (MQ=255)
aTTTTCACGCGTTAATCCGCCCTGCTCAACAAGCCACTGATAACCATCATCGGCCAACATTTGCGTCCACAGATAAGCGta < 1:304153/81‑1 (MQ=255)
cACGCGTTAATCCGCCCTGCTCAACAAGCCACTGATAACCATCATCGGCCAACATTTGCGTCCACAGATAAGCGTAATAAc > 1:304189/1‑81 (MQ=255)
cgcgTTAATCCGCCCTGCTCAACAAGCCACTGATAACCATCATCGGCCAACATTTGCGTCCACAGATAAGCGTAATAACCt < 2:304155/81‑1 (MQ=255)
cgcgTTAATCCGCCCTGCTCAACAAGCCACTGATAACCATCATCGGCCAACATTTGCGTCCACAGATAAGCGTAATAACCt < 1:304156/81‑1 (MQ=255)
cgcgTTAATCCGCCCTGCTCAACAAGCCACTGATAACCATCATCGGCCAACATTTGCGTCCACAGATAAGCGTAATAACCt < 1:304154/81‑1 (MQ=255)
gTTAATCCGCCCTGCTCAACAAGCCACTGATAACCATCATCGGCCAACATTTGCGTCCACAGATAAGCGTAATAACCTgca < 1:304157/81‑1 (MQ=255)
ttAATCCGCCCTGCTCAACAAGCCACTGATAACCATCATCGGCCAACATTTGCGTCCACAGATAAGCGTAATAACCTgcag < 2:304158/81‑1 (MQ=255)
aaTCCGCCCTGCTCAACAAGCCACTGATAACCATCATCGGCCAACATTTGCGTCCACAGATAAGCGTAATAACCTgcagca > 2:304193/1‑81 (MQ=255)
cGCCCTGCTCAACAAGCCACTGATAACCATCATCGGCCAACATTTGCGTCCACAGATAAGCGTAATAACCTGCAGCatat < 1:304159/80‑1 (MQ=255)
gCCCTGCTCAACAAGCCACTGATAACCATCATCGGCCAACATTTGCGTCCACAGATAAGCGTAATAACCTGCAGCATATcc > 2:304191/1‑81 (MQ=255)
|
TTACCTCTGGAAAGGATCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTTAATCCGCCCTGCTCAACAAACCACTGATAACCATCATCGGCCAACATTTGCGTCCACAGATAAGCGTAATAACCTGCAGCATATCC > NC_000913/1625426‑1625562
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
