Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,706,877 |
A→T |
D177V (GAT→GTT) |
rsxB → |
SoxR [2Fe‑2S] reducing system protein RsxB |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,706,877 | 0 | A | T | 100.0%
| 103.1
/ NA
| 29 | D177V (GAT→GTT) | rsxB | SoxR [2Fe‑2S] reducing system protein RsxB |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (14/15); total (14/15) |
GTTGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATGGGATCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAAT > NC_000913/1706807‑1706951
|
gtTGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATGGGTTCTGAacac > 2:316285/1‑80 (MQ=255)
ttGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATGGGTTCTGAACAcc < 2:316244/80‑1 (MQ=255)
aTCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATGGGTTCTGAACACCATTc < 1:316245/81‑1 (MQ=255)
gTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATGGGTTCTGAACACCATTCCCGt < 2:316246/81‑1 (MQ=255)
gCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATGGGTTCTGAACACCATTCCCGTGc > 2:316290/1‑81 (MQ=255)
aCTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATGGGTTCTGAACACCATTCCCGTGCGTATCATTc < 1:316247/81‑1 (MQ=255)
cTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATGGGTTCTGAACACCATTCCCGTGCGTATCATTcc < 1:316248/81‑1 (MQ=255)
cTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATGGGTTCTGAACACCATTCCCGTGCGTATCATTcc > 1:316274/1‑81 (MQ=255)
cTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATGGGTTCTGAACACCATTCCCGTGCGTATCATTcc > 2:316281/1‑81 (MQ=255)
cATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATGGGTTCTGAACACCATTCCCGTGCGTATCATTCCCg < 1:316250/80‑1 (MQ=255)
cATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATGGGTTCTGAACACCATTCCCGTGCGTATCATTCCCGt < 2:316249/81‑1 (MQ=255)
tctcGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATGGGTTCTGAACACCATTCCCGTGCGTATCATTCCCGTg > 1:316278/1‑80 (MQ=255)
gTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATGGGTTCTGAACTCCATTCCCGTGCGTATCATTCCCGTGGAaca > 2:316280/1‑81 (MQ=255)
tGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATGGGTTCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAacac > 2:316282/1‑80 (MQ=255)
gCAACCGGTCGCAGAAACACCTGACTCCTGGAAATGGGTTCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCa > 1:316284/1‑81 (MQ=255)
aaCCGGTCGCAGAAACACCTGACTCCTGGAAATGGGTTCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATg < 1:316251/81‑1 (MQ=255)
aCCGGTCGCAGAAACACCTGACTCCTGGAAATGGGTTCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGc > 1:316277/1‑81 (MQ=255)
ccGGTCGCAGAAACACCTGACTCCTGGAAATGGGTTCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCt < 1:316253/81‑1 (MQ=255)
ccGGTCGCAGAAACACCTGACTCCTGGAAATGGGTTCTGAACACCATTCCCGTGCGTAACATTCCCGTGGAACACCATGCt < 2:316252/81‑1 (MQ=255)
cGGTCGCAGAAACACCTGACTCCTGGAAATGGGTTCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCtt < 1:316254/81‑1 (MQ=255)
gTCGCAGAAACACCTGACTCCTGGAAATGGGTTCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTaa < 1:316255/81‑1 (MQ=255)
aaCACCTGACTCCTGGAAATGGGTTCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATtct > 1:316283/1‑81 (MQ=255)
cacCTGACTCCTGGAAATGGGTTCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTctct < 2:316256/81‑1 (MQ=255)
acCTGACTCCTGGAAATGGGTTCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTg > 2:316287/1‑81 (MQ=255)
ccTGACTCCTGGAAATGGGTTCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGc < 2:316257/81‑1 (MQ=255)
aCTCCTGGAAATGGGTTCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCAtt < 1:316258/80‑1 (MQ=255)
cTCCTGGAAATGGGTTCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCAtt > 1:316296/1‑79 (MQ=255)
gAAATGGGTTCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGaaaa > 1:316286/1‑80 (MQ=255)
aTGGGTTCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAaaaat > 2:316279/1‑80 (MQ=255)
|
GTTGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATGGGATCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAAT > NC_000913/1706807‑1706951
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
