Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,748,585 |
T→A |
R485R (CGT→CGA) |
ydhS → |
FAD/NAD(P) binding domain‑containing protein YdhS |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,748,585 | 0 | T | A | 100.0%
| 120.4
/ NA
| 34 | R485R (CGT→CGA) | ydhS | FAD/NAD(P) binding domain‑containing protein YdhS |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (19/15); total (19/15) |
CGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGTGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATGCACGACCAGCCTTTCGTTCAGGGACTTACGGCATGTG > NC_000913/1748507‑1748658
|
cgAGCAATTACAGAAAACAGGGGATGAAGTCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGAgg < 2:323001/81‑1 (MQ=255)
aaTTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGAGGGcgcg < 2:323002/81‑1 (MQ=255)
aTTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGAGGGCGCGt < 1:323003/81‑1 (MQ=255)
gAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGAGGGCGCGTAGCGtt > 1:323057/1‑81 (MQ=255)
aaaaCAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGAGGGCGCGTAGCGTTc < 1:323004/81‑1 (MQ=255)
aaaCAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGAGGGCGCGTAGCGTTCg > 1:323049/1‑81 (MQ=255)
aaaCAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGAGGGCGCGTAGCGTGCg > 2:323048/1‑81 (MQ=255)
aCAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGAGGGCGCGTAGCGTTCGgc < 2:570477/81‑1 (MQ=255)
aCAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGAGGGCGCGTAGCGTTCGgc < 1:323005/81‑1 (MQ=255)
aGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGAGGGCGCGTAGCGTTCGgcgc > 2:323044/1‑81 (MQ=255)
aGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGAGGGCGCGTAGCGTTCGgcgc > 1:323042/1‑81 (MQ=255)
ggATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGAGGGCGCGTAGCGTTCGGCGCGtt < 2:323007/81‑1 (MQ=255)
ggATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGAGGGCGCGTAGCGTTCGGCGCGtt < 1:323006/81‑1 (MQ=255)
aaaTCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGAGGGCGCGTAGCGTTCGGCGCGTTGCCCt < 2:323008/81‑1 (MQ=255)
tCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGAGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGt > 2:323052/1‑81 (MQ=255)
ccTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGAGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGtt > 1:323039/1‑80 (MQ=255)
cTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGAGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGa > 2:323051/1‑81 (MQ=255)
ttGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGAGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATGCAc > 1:323058/1‑80 (MQ=255)
ttGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGAGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATGCAc < 2:323010/80‑1 (MQ=255)
ttGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGAGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATGCACg < 2:323009/81‑1 (MQ=255)
tGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGAGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATGCACGa > 2:323041/1‑81 (MQ=255)
ggCGAAGATTATACGTTACAGCAACCCGAAGATATTCGAGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATGCACGAc > 2:323050/1‑81 (MQ=255)
ggCGAAGATTATACGTTACAGCAACCCGAAGATATTCGAGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATGCACGAc > 2:323061/1‑81 (MQ=255)
gAAGATTATACGTTACAGCAACCCGAAGATATTCGAGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATGCACGACCAg < 1:323011/81‑1 (MQ=255)
ttATACGTTACAGCAACCCGAAGATATTCGAGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATGCACGACCAGCCttt < 2:323012/81‑1 (MQ=255)
ataCGTTACAGCAACCCGAAGATATTCGAGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATGCACGACCAGCCTTTc < 2:323013/80‑1 (MQ=255)
taCGTTACAGCAACCCGAAGATATTCGAGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATGCACGACCAGCCTTTCGt < 1:323014/81‑1 (MQ=255)
aCAGCAACCCGAAGATATTCGAGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATGCACGACCAGCCTTTCGTTCAgg > 1:323059/1‑80 (MQ=255)
cGAAGATATTCGAGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATGCACGACCAGCCTTTCGTTCAGGGACTTACgg > 1:323053/1‑80 (MQ=255)
gAAGATATTCGAGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATGCACGACCAGCCTTTCGTTCAGGGACTTACGGCa > 2:323054/1‑81 (MQ=255)
gAAGATATTCGAGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATGCACGACCAGCCTTTCGTTCAGGGACTTACGGCa > 2:323076/1‑81 (MQ=255)
aGATATTCGAGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATGCACGACCAGCCTTTCGTTCAGGGACTTACGGCAtg > 1:323040/1‑81 (MQ=255)
tatTCGAGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATGCACGACCAGCCTTTCGTTCAGGGACTTACGGCAtgtg > 1:323063/1‑80 (MQ=255)
atTCGAGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATGCACGACCAGCCTTTCGTTCAGGGACTTACGGCAtgtg > 2:323045/1‑79 (MQ=255)
|
CGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGTGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATGCACGACCAGCCTTTCGTTCAGGGACTTACGGCATGTG > NC_000913/1748507‑1748658
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
