Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,810,695 |
T→C |
M162T (ATG→ACG) |
ydjM → |
putative inner membrane protein regulated by LexA |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,810,695 | 0 | T | C | 100.0%
| 113.1
/ NA
| 31 | M162T (ATG→ACG) | ydjM | putative inner membrane protein regulated by LexA |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (15/16); total (15/16) |
ATCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGTATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCA > NC_000913/1810622‑1810770
|
aTCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGTATGGACGCCCcat > 2:332904/1‑81 (MQ=255)
aTCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGTATGGACGCCCcat > 1:332902/1‑81 (MQ=255)
tCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGTATGGACGCCCcat < 1:332866/80‑1 (MQ=255)
tCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGTATGGACGCCCca < 1:332868/79‑1 (MQ=255)
tCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGAATTATTTGTCTGGTCGGTATGGACGCCCca < 1:332867/79‑1 (MQ=255)
ccTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGTATGGACGCCCcatt > 2:332905/1‑80 (MQ=255)
ccTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGTATGGACGCCCcatt > 1:332898/1‑80 (MQ=255)
gTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGTATGGACGCCCcattcatt < 1:332869/80‑1 (MQ=255)
gTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGTATGGACGCCCcattcat < 1:332870/79‑1 (MQ=255)
cccAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGTATGGACGCCCCATTCATTAccc > 1:332901/1‑80 (MQ=255)
cccAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGTATGGACGCCCCATTCATTACCCg < 1:332871/81‑1 (MQ=255)
cccAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGTATGGACGCCCCATTCATTACCCg < 1:332872/81‑1 (MQ=255)
cAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGCTCGGTATGGACGCCCCATTCATTAccc > 1:332889/1‑78 (MQ=255)
aaGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTTGGTATGGACGCCCCATTCATTACCCGAGaa > 2:332907/1‑80 (MQ=255)
aGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGTATGGACGCCCCATTCATTACCCGAGaa > 2:332900/1‑79 (MQ=255)
aaccaacTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGTATGGACGCCCCATTCATTACCCGAGAACAGCGCt < 1:332873/81‑1 (MQ=255)
aacTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGTATGGACGCCCCATTCATTACCCGAGAACAGCGCTGTTc < 2:332874/81‑1 (MQ=255)
gTTTTATCTGCATGGCATTATTTGTCTGGTCGGTATGGACGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCAt < 2:332875/81‑1 (MQ=255)
tttATCTGCATGGCATTATTTGTCTGGTCGGTATGGACGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCg > 1:332906/1‑81 (MQ=255)
tATCTGCATGGCATTATTTGTCTGGTCGGTATGGACGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGc < 1:332876/80‑1 (MQ=255)
aTCTGCATGGCATTATTTGTCTGGTCGGTATGGACGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCa < 1:332877/80‑1 (MQ=255)
cATGGCATTATTTGTCTGGTCGGTATGGACGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCaaa < 1:332880/77‑1 (MQ=255)
cATGGCATTATTTGTCTGGTCGGTATGGACGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGAt < 1:332878/81‑1 (MQ=255)
cATGGCATTATTTGTCTGGTCGGTATGGACGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGAt < 2:332879/81‑1 (MQ=255)
aTGGCATTATTTGTCTGGTCGGTATGGACGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGAt > 1:332909/1‑80 (MQ=255)
attattTGTCTGGTCGGTATGGACGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATAc > 2:332903/1‑81 (MQ=255)
ttattTGTCTGGTCGGTATGGACGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATAcc > 1:332899/1‑81 (MQ=255)
tattTGTCTGGTCGGTATGGACGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCt > 1:332917/1‑81 (MQ=255)
gTCGGTATGGACGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATc > 1:332908/1‑79 (MQ=255)
gTCGGTATGGACGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATc < 1:332881/79‑1 (MQ=255)
aTGGACGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCa > 1:332915/1‑81 (MQ=255)
|
ATCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGTATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCA > NC_000913/1810622‑1810770
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
