Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,855,607 |
A→T |
L122* (TTG→TAG) |
ydjG ← |
NADH‑dependent methylglyoxal reductase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,855,607 | 0 | A | T | 100.0%
| 105.7
/ NA
| 29 | L122* (TTG→TAG) | ydjG | NADH‑dependent methylglyoxal reductase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (17/12); total (17/12) |
GCGATCGGCGTAAAAAATGGCGGCACCGACTGCCAGTGCGTCATGTAGATATCGATGTAATCAATACCCAGACGTTGCAAGCTCGCTGCTACCTCTTCGCGGATAGATTCCGGGGAAAGGTTTTTATACAACTGCCGATCGCCAACTTTGTTGA > NC_000913/1855529‑1855682
|
gCGATCGGCGTAAAAAATGGCGGCACCGACTGCCAGTGCGTCATGTAGATATCGATGTAATCAATACCCAGACGTTGCTAg < 1:340391/81‑1 (MQ=255)
gATCGGCGTAAAAAATGGCGGCACCGACTGCCAGTGCGTCATGTAGATATCTATGTAATCAATACCCAGACGTTGCTAGCt > 2:340436/1‑81 (MQ=255)
gATCGGCGTAAAAAATGGCGGCACCGACTGCCAGTGCGTCATGTAGATATCGATGTAATCAATACCCAGACGTTGCTAGCt > 2:340429/1‑81 (MQ=255)
tCGGCGTAAAAAATGGCGGCACCGACTGCCAGTGCGTCATGTAGATATCGATGTAATCAATACCCAGACGTTGCTAGCTc > 1:340392/1‑80 (MQ=255)
cGGCGTAAAAAATGGCGGCACCGACTGCCAGTGCGTCATGTAGATATCGATGTAATCAATACCCAGACGTTGCTAGCTCgc > 2:340421/1‑81 (MQ=255)
cGGCGTAAAAAATGGCGGCACCGACTGCCAGTGCGTCATGTAGATATCGATGTAATCAATACCCAGACGTTGCTAGCTCgc > 1:340423/1‑81 (MQ=255)
ggCGTAAAAAATGGCGGCACCGACTGCCAGTGCGTCATGTAGATATCGATGTAATCAATACCCAGACGTTGCTAGCTCgct > 1:340428/1‑81 (MQ=255)
aaTGGCGGCACCGACTGCCAGTGCGTCATGTAGATATCGATGTAATCAATACCCAGACGTTGCTAGCTCGCTGCTACctct > 2:340435/1‑81 (MQ=255)
gcggcACCGACTGCCAGTGCGTCATGTAGATATCGATGTAATCAATACCCAGACGTTGCTAGCTCGCTGCTACCTCTTcgc > 1:340427/1‑81 (MQ=255)
cACCGACTGCCAGTGCGTCATGTAGATATCTATGTAATCAATACCCAGACGTTGCTAGCTCGCTGCTACCTCTTCGCGGa > 1:340439/1‑80 (MQ=255)
aCCGACTGCCAGTGCGTCATGTAGATATCTATGTAATCAATACCCAGACGTTGCTAGCTCGCTGCTACCTCTTCGCGGa > 2:340430/1‑79 (MQ=255)
ccGACTGCCAGTGCGTCATGTAGATATCGATGTAATCAATACCCAGACGTTGCTAGCTCGCTGCTACCTCTTCGCGGATAg < 2:340393/81‑1 (MQ=255)
cTGCCAGTGCGTCATGTAGATATCGATGTAATCAATACCCAGACGTTGCTAGCTCGCTGCTACCTCTTCGCGGATAGATTc < 1:340394/81‑1 (MQ=255)
tGCCAGTGCGTCATGTAGATATCGATGTAATCAATACCCAGACGTTGCTAGCTCGCTGCTACCTCTTCGCGGATAGATTcc > 2:340426/1‑81 (MQ=255)
gTGCGTCATGTAGATATCGATGTAATCAATACCCAGACGTTGCTAGCTCGCTGCTACCTCTTCGCGGATAGATTCCgggg > 1:340425/1‑80 (MQ=255)
tGTAGATATCGATGTAATCAATACCCAGACGTTGCTAGCTCGCTGCTACCTCTTCGCGGATAGATTCCGGGGAAAGGtttt < 2:340395/81‑1 (MQ=255)
gATATCGATGTAATCAATACCCAGACGTTGCTAGCTCGCTGCTACCTCTTCGCGGATAGATTCCGGGGAAAGGTTTTtata > 1:340434/1‑81 (MQ=255)
tCGATGTAATCAATACCCAGACGTTGCTAGCTCGCTGCTACCTCTTCGCGGATAGATTCCGGGGAAAGGTTTTTATACAAc < 1:340396/81‑1 (MQ=255)
aTCAATACCCAGACGTTGCTAGCTCGCTGCTACCTCTTCGCGGATAGATTCCGGGGAAAGGTTTTTATACAACTGCCGAt < 2:340397/80‑1 (MQ=255)
tCAATACCCAGACGTTGCTAGCTCGCTGCTACCTCTTCGCGGATAGATTCCGGGGAAAGGTTTTTATACAACTGCCGATCg > 2:340398/1‑81 (MQ=255)
aaTACCCAGACGTTGCTAGCTCGCTGCTACCTCTTCGCGGATAGATTCCGGGGAAAGGTTTTTATACAACTGCCGATCGcc < 2:340401/81‑1 (MQ=255)
aaTACCCAGACGTTGCTAGCTCGCTGCTACCTCTTCGCGGATAGATTCCGGGGAAAGGTTTTTATACAACTGCCGATCGcc < 1:340399/81‑1 (MQ=255)
aaTACCCAGACGTTGCTAGCTCGCTGCTACCTCTTCGCGGATAGATTCCGGGGAAAGGTTTTTATACAACTGCCGATCGc > 2:340431/1‑80 (MQ=255)
aaTACCCAGACGTTGCTAGCTCGCTGCTACCTCTTCGCGGATAGATTCCGGGGAAAGGTTTTTATACAACTGCCGATCGc < 1:340400/80‑1 (MQ=255)
cccAGACGTTGCTAGCTCGCTGCTACCTCTTCGCGGATAGATTCCGGGGAAAGGTTTTTATACAACTGCCGATCGCCAACt > 1:340432/1‑81 (MQ=255)
ccAGACGTTGCTAGCTCGCTGCTACCTCTTCGCGGATAGATTCCGGGGAAAGGTTTTTATACAACTGCCGATCGCCAACtt < 2:340402/81‑1 (MQ=255)
gACGTTGCTAGCTCGCTGCTACCTCTTCGCGGATAGATTCCGGGGAAAGGTTTTTATACAACTGCCGATCGCCAACTttgt < 1:340403/81‑1 (MQ=255)
gTTGCTAGCTCGCTGCTACCTCTTCGCGGATAGATTCCGGGGAAAGGTTTTTATACAACTGCCGATCGCCAACTttgttg > 1:340438/1‑80 (MQ=255)
gTTGCTAGCTCGCTGCTACCTCTTCGCGGATAGATTCCGGGGAAAGGTTTTTATACAACTGCCGATCGCCAACTTTGTTGa < 1:340404/81‑1 (MQ=255)
|
GCGATCGGCGTAAAAAATGGCGGCACCGACTGCCAGTGCGTCATGTAGATATCGATGTAATCAATACCCAGACGTTGCAAGCTCGCTGCTACCTCTTCGCGGATAGATTCCGGGGAAAGGTTTTTATACAACTGCCGATCGCCAACTTTGTTGA > NC_000913/1855529‑1855682
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
