Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,900,559 |
C→A |
E343* (GAA→TAA) |
yoaE ← |
putative inner membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,900,559 | 0 | C | A | 100.0%
| 107.5
/ NA
| 30 | E343* (GAA→TAA) | yoaE | putative inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (10/20); total (10/20) |
GAAGCAATCGCCGCCACATCAACGCCCTCTTCCAGCGCCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTCATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGACCCC > NC_000913/1900483‑1900634
|
gAAGCAATCGCCGCCACATCAACGCCCTCTTCCAGCGCCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTAATcc < 2:347687/81‑1 (MQ=255)
gAAGCAATCGCCGCCACATCAACGCCCTCTTCCAGCGCCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTAATcc < 2:347686/81‑1 (MQ=255)
aGCAATCGCCGCCACATCAACGCCCTCTTCCAGCGCCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTAATCCAg < 1:347688/81‑1 (MQ=255)
gCAATCGCCGCCACATCAACGCCCTCTTCCAGCGCCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTAATCCAGt < 1:347689/81‑1 (MQ=255)
aTCGCCGCCACATCAACGCCCTCTTCCAGCGCCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTAATCCAGTTCa > 2:347747/1‑81 (MQ=255)
gccgccACATCAACGCCCTCTTCCAGCGCCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTAATCCAGTTCACcg < 1:347690/81‑1 (MQ=255)
gccACATCAACGCCCTCTTCCAGCGCCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTAATCCAGTTCACCTCGa > 2:347749/1‑81 (MQ=255)
acaTCAACGCCCTCTTCCAGCGCCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTAATCCAGTTCACCGCGACAt < 2:347691/81‑1 (MQ=255)
aaCGCCCTCTTCCAGCGCCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTAATCCAGTTCACCGCGACATACCg < 1:347692/80‑1 (MQ=255)
cGCCCTCTTCCAGCGCCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTAATCCAGTTCACCGCGACATACCGGGa < 1:347693/81‑1 (MQ=255)
ctTCCAGCGCCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTAATCCAGTTCACCGCGACATACCGGGAACAGAc > 1:347729/1‑81 (MQ=255)
ccAGCGCCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTAATCCAGTTCACCGCGACATACCGGGAACAGACt < 2:347694/79‑1 (MQ=255)
cAGCGCCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTAATCCAGTTCACCGCGACATACCGGGAACAGACtgtg > 2:347731/1‑81 (MQ=255)
cAGCGCCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTAATCCAGTTCACCGCGACATACCGGGAACAGACtgt < 1:347695/80‑1 (MQ=255)
gcgcCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTAATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCg < 1:347696/81‑1 (MQ=255)
ccaccaGCAGTTCTTTAGCACGTACAATACCGATGATTTAATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTg < 2:347697/81‑1 (MQ=255)
accaGCAGTTCTTTAGCACGTACAATACCGATGATTTAATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGaa < 2:347698/81‑1 (MQ=255)
ccggcagTTCTTTAGCACGTACAATACCGATGATTTAATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAag < 2:347699/78‑1 (MQ=255)
agTTCTTTAGCACGTACAATACCGATGATTTAATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAagagag > 1:347739/1‑80 (MQ=255)
cTTTAGCACGTACAATACCGATGATTTAATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGtt > 2:347757/1‑81 (MQ=255)
aGCACGTACAATACCGATGATTTAATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCt < 2:347700/80‑1 (MQ=255)
aGCACGTACAATACCGATGATTTAATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTc > 1:347751/1‑81 (MQ=255)
aGCACGTACAATACCGATGATTTAATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTc > 2:347750/1‑81 (MQ=255)
cACGTACAATACCGATGATTTAATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTcgc < 2:347702/81‑1 (MQ=255)
cACGTACAATACCGATGATTTAATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTcgc < 1:347701/81‑1 (MQ=255)
aCAATACCGATGATTTAATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGAtt > 2:347752/1‑81 (MQ=255)
cAATACCGATGATTTAATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGAtt < 1:347703/80‑1 (MQ=255)
tACCGATGATTTAATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCAt < 1:347704/81‑1 (MQ=255)
ccGATGATTTAATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATc < 1:347705/80‑1 (MQ=255)
gatTTAATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGAcccc > 1:347760/1‑81 (MQ=255)
|
GAAGCAATCGCCGCCACATCAACGCCCTCTTCCAGCGCCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTCATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGACCCC > NC_000913/1900483‑1900634
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
