Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,907,906 |
C→T |
intergenic (‑315/+355) |
yobF ← / ← yebO |
DUF2527 domain‑containing protein YobF/uncharacterized protein YebO |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,907,906 | 0 | C | T | 100.0%
| 55.7
/ NA
| 16 | intergenic (‑315/+355) | yobF/yebO | DUF2527 domain‑containing protein YobF/uncharacterized protein YebO |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (3/13); total (3/13) |
AGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAAAGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCAATCCCCAAAGGGATTGT > NC_000913/1907829‑1907977
|
aGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAAAGTTATATCAtttt > 2:348969/1‑81 (MQ=255)
ggCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAAAGTTATATCATtttt < 2:348952/81‑1 (MQ=255)
cAGTGGTAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAAAGTTATATCATTTTTtct < 1:348953/81‑1 (MQ=255)
gagaAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAAAGTTATATCATTTTTTCTCATaa > 2:348971/1‑80 (MQ=255)
gagaAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAAAGTTATATCATTTTTTCTCATaa > 1:348970/1‑80 (MQ=255)
attaCATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAAAGTTATATCATTTTTTCTCATAAAAcagtca < 2:348955/80‑1 (MQ=255)
attaCATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAAAGTTATATCATTTTTTCTCATAAAAcagtca < 2:348954/80‑1 (MQ=255)
cgtgcAATATCACATTTTTTGCTATGCAATGAATAAAAAGTTATATCATTTTTTCTCATAAAACAGTCAGTTAACGGCTat < 1:348956/81‑1 (MQ=255)
ttGCTATGCAATGAATAAAAAGTTATATCATTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAgaga < 1:348957/80‑1 (MQ=255)
tGCTATGCAATGAATAAAAAGTTATATCATTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGaa < 2:348958/80‑1 (MQ=255)
gCTATGCAATGAATAAAAAGTTATATCATTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAg < 2:348959/80‑1 (MQ=255)
gCTATGCAATGAATAAAAAGTTATATCATTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAg < 1:348960/80‑1 (MQ=255)
aTGCAATGAATAAAAAGTTATATCATTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCaa > 2:348972/1‑81 (MQ=255)
aaTGAATAAAAAGTTATATCATTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCAATccc < 2:348961/81‑1 (MQ=255)
aTAAAAAGTTATATCATTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCAATCCCCAAAg < 1:348962/81‑1 (MQ=255)
aaaGTTATATCATTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCAATCCCCAAAGGGAt < 1:348963/81‑1 (MQ=255)
gTTATATCATTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCAATCCCCAAAGGGATTGt < 2:348964/81‑1 (MQ=255)
|
AGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAAAGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCAATCCCCAAAGGGATTGT > NC_000913/1907829‑1907977
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
