Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,082,090 |
T→C |
N153S (AAC→AGC) |
dacD ← |
D‑alanyl‑D‑alanine carboxypeptidase DacD |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,082,090 | 0 | T | C | 100.0%
| 75.5
/ NA
| 22 | N153S (AAC→AGC) | dacD | D‑alanyl‑D‑alanine carboxypeptidase DacD |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (11/11); total (11/11) |
ATGCTGGCCAGGTGCATCCAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACCGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATC > NC_000913/2082014‑2082166
|
aTGCTGGCCAGGTGCATCCAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGCtcat > 2:378954/1‑81 (MQ=255)
gCTGGCCAGGTGCATCCAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGCtcatca > 2:378963/1‑81 (MQ=255)
gCCAGGTGCATCCAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGCTCATCAtt < 1:378924/79‑1 (MQ=255)
gCCAGGTGCATCCAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGCTCATCATTTc < 1:378925/81‑1 (MQ=255)
gCATCCAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGCTCATCATTTCAACAAAc > 1:378958/1‑81 (MQ=255)
cATCCAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGCTCATCATTTCAACAAACt < 2:378926/81‑1 (MQ=255)
cAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGCTCATCATTTCAACAAACTGCCg > 2:378965/1‑81 (MQ=255)
cAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGCTCATCATTTCAACAAACTGCCg > 2:378956/1‑81 (MQ=255)
aCCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGCTCATCATTTCAACAAACTGCCGTTg < 2:378927/81‑1 (MQ=255)
ccATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGCTCATCATTTCAACAAACTGCCGTTGc > 2:378962/1‑81 (MQ=255)
aTGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGCTCATCATTTCAACAAACTGCCGTTGccc < 2:378928/81‑1 (MQ=255)
aTGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGCTCATCATTTCAACAAACTGCCGTTGccc < 1:378929/81‑1 (MQ=255)
gCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGCTCATCATTTCAACAAACTGCCGTTGCCCAc > 1:378966/1‑81 (MQ=255)
cACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGCTCATCATTTCAACAAACTGCCGTTGCCCAcc < 2:378930/81‑1 (MQ=255)
tttCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGCTCATCATTTCAACAAACTGCCGTTGCCCACCGGCa < 1:378931/80‑1 (MQ=255)
aaaaTGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGCTCATCATTTCAACAAACTGCCGTTGCCCACCGGCAAtata < 2:378932/81‑1 (MQ=255)
aaaTGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGCTCATCATTTCAACAAACTGCCGTTGCCCACCGGCAATATAg < 2:378933/81‑1 (MQ=255)
aTGCATATCCTTGAGATGCAGCTTCTCGGCATAGTTGCTCATCATTTCAACAAACTGCCGTTGCCCACCGGCAATATAGt > 1:378960/1‑80 (MQ=255)
gagaTGCAGCTTCTCGGCATAGTTGCTCATCATTTCAACAAACTGCCGTTGCCCACCGGCAATATAGTCAGCCAGAGCaa > 2:378973/1‑80 (MQ=255)
cAGCTTCTCGGCATAGTTGCTCATCATTTCAACAAACTGCCGTTGCCCACCGGCAATATAGTCAGCCAGAGCAACACAAGc < 1:378934/81‑1 (MQ=255)
tctcGGCATAGTTGCTCATCATTTCAACAAACTGCCGTTGCCCACCGGCAATATAGTCAGCCAGAGCAACACAAGCGTCAt > 2:378967/1‑81 (MQ=255)
cATAGTTGCTCATCATTTCAACAAACTGCCGTTGCCCACCGGCAATATAGTCAGCCAGAGCAACACAAGCGTTATTTCCg > 1:378990/1‑80 (MQ=255)
ttgctCATCATTTCAACAAACTGCCGTTGCCCACCGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATc > 1:378978/1‑80 (MQ=255)
|
ATGCTGGCCAGGTGCATCCAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACCGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATC > NC_000913/2082014‑2082166
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
