Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,240,203 |
T→A |
Q49L (CAA→CTA) |
mglB ← |
D‑galactose/methyl‑galactoside ABC transporter periplasmic binding protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,240,203 | 0 | T | A | 100.0%
| 110.3
/ NA
| 31 | Q49L (CAA→CTA) | mglB | D‑galactose/methyl‑galactoside ABC transporter periplasmic binding protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (6/25); total (6/25) |
ATCGTTCTGCTTGGACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTTGCTCAATAGCCTTGCGCACTACAGACATAAAGTTATCGTCGTACTTATAGATTGTTACACCAATGCGAG > NC_000913/2240130‑2240272
|
aTCGTTCTGCTTGGACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTAGCTCAAt < 1:407140/81‑1 (MQ=255)
aTCGTTCTGCTTGGACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTAGCTCAAt < 2:407139/81‑1 (MQ=255)
gTTCTGCTTGGACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTAGCTCAATAGc > 1:407190/1‑81 (MQ=255)
cTGCTTGGACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTAGCTCAATAGCCtt > 1:407180/1‑81 (MQ=255)
ttGGACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTAGCTCAATAGCCTTgcg > 2:407195/1‑80 (MQ=255)
ggACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTAGCTCAATAGCCTTgcgc < 2:407143/79‑1 (MQ=255)
ggACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTAGCTCAATAGCCTTGCGCa < 1:407141/80‑1 (MQ=255)
ggACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTAGCTCAATAGCCTTGCGCa < 2:407142/80‑1 (MQ=255)
gACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTAGCTCAATAGCCTTGCGCAc < 1:407145/80‑1 (MQ=255)
gACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTAGCTCAATAGCCTTGCGCACt < 2:407144/81‑1 (MQ=255)
gTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTAGCTCAATAGCCTTGCGCACTACAg < 2:407146/80‑1 (MQ=255)
aTTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTAGCTCAATAGCCTTGCGCACTACAGACAt < 1:407148/81‑1 (MQ=255)
aTTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTAGCTCAATAGCCTTGCGCACTACAGACAt < 2:407147/81‑1 (MQ=255)
cTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTAGCTCAATAGCCTTGCGCACTACAGACATaa < 2:407149/80‑1 (MQ=255)
agaATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTAGCTCAATAGCCTTGCGCACTACAGACATAAAGtt < 1:407150/81‑1 (MQ=255)
gaATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTAGCTCAATAGCCTTGCGCACTACAGACATAAAGTTa < 1:407152/81‑1 (MQ=255)
gaATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTAGCTCAATAGCCTTGCGCACTACAGACATAAAGTTa < 1:407151/81‑1 (MQ=255)
gaATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTAGCTCAATAGCCTTGCGCACTACAGACATAAAGTTa < 1:407153/81‑1 (MQ=255)
aaTCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTAGCTCAATAGCCTTGCGCACTACAGACATAAAGTTAt < 1:407154/81‑1 (MQ=255)
catcaGCAGCTGAACATCTGGCGCGGCTTTCGCATCTAGCTCAATAGCCTTGCGCACTACAGACATAAAGTTATcgtcgt < 2:407155/80‑1 (MQ=255)
catcaGCAGCTGAACATCTGGCGCGGCTTTCGCATCTAGCTCAATAGCCTTGCGCACTACAGACATAAAGTTATcgtcgt < 2:407156/80‑1 (MQ=255)
agcagcTGAACATCTGGCGCGGCTTTCGCATCTAGCTCAATAGCCTTGCGCACTACAGACATAAAGTTATCGTCGTACTta < 1:407157/81‑1 (MQ=255)
agcagcTGAACATCTGGCGCGGCTTTCGCATCTAGCTCAATAGCCTTGCGCACTACAGACATAAAGTTATCGTCGTACTta > 1:407193/1‑81 (MQ=255)
tGAACATCTGGCGCGGCTTTCGCATCTAGCTCAATAGCCTTGCGCACTATAGACATAAAGTTATCGTCGTACTTATAGAtt > 2:407183/1‑81 (MQ=255)
aaCATCTGGCGCGGCTTTCGCATCTAGCTCAATAGCCTTGCGCACTACAGACATAAAGTTATCGTCGTACTTATAGAtt < 1:407159/79‑1 (MQ=255)
aaCATCTGGCGCGGCTTTCGCATCTAGCTCAATAGCCTTGCGCACTACAGACATAAAGTTATCGTCGTACTTATAGATTGt < 2:407158/81‑1 (MQ=255)
aaCATCTGGCGCGGCTTTCGCATCTAGCTCAATAGCCTTGCGCACTACAGACATAAAGTTATCGTCGTACTTATAGATTGt < 2:407160/81‑1 (MQ=255)
gcgcGGCTTTCGCATCTAGCTCAATAGCCTTGCGCACTACAGACATAAAGTTATCGTCGTACTTATAGATTGTTaca < 1:407161/77‑1 (MQ=255)
cTTTCGCATCTAGCTCAATAGCCTTGCGCACTACAGACATAAAGTTATCGTCGTACTTATAGATTGTTACACCAATGCGAg < 2:407162/81‑1 (MQ=255)
cTTTCGCATCTAGCTCAATAGCCTTGCGCACTACAGACATAAAGTTATCGTCGTACTTATAGATTGTTACACCAATGCGAg < 2:407163/81‑1 (MQ=255)
tttCGCATCTAGCTCAATAGCCTTGCGCACTACAGACATAAAGTTATCGTCGTACTTATAGATTGTTACACCAATGCGAg > 2:407191/1‑80 (MQ=255)
|
ATCGTTCTGCTTGGACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTTGCTCAATAGCCTTGCGCACTACAGACATAAAGTTATCGTCGTACTTATAGATTGTTACACCAATGCGAG > NC_000913/2240130‑2240272
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
