Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,460,346 |
T→A |
K42* (AAG→TAG) |
fadI ← |
3‑ketoacyl‑CoA thiolase FadI |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,460,346 | 0 | T | A | 100.0%
| 114.3
/ NA
| 32 | K42* (AAG→TAG) | fadI | 3‑ketoacyl‑CoA thiolase FadI |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (12/20); total (12/20) |
CGACCTGACCAAAGACCAGTTGTTCAATCACTTCGGCGGGGATCTCGCTGCGTGCCAGCAGTTCGCCTACCACCATCTTCCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTCGCCTGACGGGCAAAAGGCGTACGTAAACCGCTAACAATGG > NC_000913/2460268‑2460423
|
cGACCTGACCAAAGACCAGTTGTTCAATCACTTCGGCGGGGATCTCGCTGCGTGCCAGCAGTTCGCCTACCACCATCTAcc > 2:449158/1‑81 (MQ=255)
cGACCTGACCAAAGACCAGTTGTTCAATCACTTCGGCGGGGATCTCGCTGCGTGCCAGCAGTTCGCCTACCACCATCTAcc > 1:449180/1‑81 (MQ=255)
aCCTGACCAAAGACCAGTTGTTCAATCACTTCGGCGGGGATCTCGCTGCGTGCCAGCAGTTCGCCTACCACCATCTACCCt < 2:449136/81‑1 (MQ=255)
ccTGACCAAAGACCAGTTGTTCAATCACTTCGGCGGGGATCTCGCTGCGTGCCAGCAGTTCGCCTACCACCATCTACCCTa < 1:449137/81‑1 (MQ=255)
aCCAAAGACCAGTTGTTCAATCACTTCGGCGGGGATCTCGCTGCGTGCCAGCAGTTCGCCTACCACCATCTACCCTAAATc < 2:449138/81‑1 (MQ=255)
cAAAGACCAGTTGTTCAATCACTTCGGCGGGGATCTCGCTGCGTGCCAGCAGTTCGCCTACCACCATCTACCCTAAATCaa < 1:449139/81‑1 (MQ=255)
aaaGACCAGTTGTTCAATCACTTCGGCGGGGATCTCGCTGCGTGCCAGCAGTTCGCCTACCACCATCTACCCTAAATCAAc < 2:449141/81‑1 (MQ=255)
aaaGACCAGTTGTTCAATCACTTCGGCGGGGATCTCGCTGCGTGCCAGCAGTTCGCCTACCACCATCTACCCTAAATCAAc < 2:449140/81‑1 (MQ=255)
aaGACCAGTTGTTCAATCACTTCGGCGGGGATCTCGCTGCGTGCCAGCAGTTCGCCTACCACCATCTACCCTAAATCAAcc < 1:449142/81‑1 (MQ=255)
aTCACTTCGGCGGGGATCTCGCTGCGTGCCAGCAGTTCGCCTACCACCATCTACCCTAAATCAACCGCGGGAATGCCATGa < 1:449143/81‑1 (MQ=255)
cggcggGGATCTCGCTGCGTGCCAGCAGTTCGCCTACCACCATCTACCCTAAATCAACCGCGGGAATGCCATGAAAAGCCg > 2:449196/1‑81 (MQ=255)
cggGGATCTCGCTGCGTGCCAGCAGTTCGCCTACCACCATCTACCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTCg > 2:449189/1‑81 (MQ=255)
gggATCTCGCTGCGTGCCAGCAGTTCGCCTACCACCATCTACCCTAGATCAACCGCGGGAATGCCATGAAAAGCCGTCGcc < 1:449144/81‑1 (MQ=255)
gggATCTCGCTGCGTGCCAGCAGTTCGCCTACCACCATCTACCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTCGcc < 1:449145/81‑1 (MQ=255)
aTCTCGCTGCGTGCCAGCAGTTCGCCTACCACCATCTACCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTCGCCTGa > 1:449186/1‑81 (MQ=255)
ctcGCTACGTGCCAGCAGATCGCCTACCACCGTCTACCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTCGCCTGAc < 2:449146/80‑1 (MQ=255)
gCTGCGTGCCAGCAGTTCGCCTACCACCATCTACCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTCGCCTGACg < 2:449147/78‑1 (MQ=255)
gCGTGCCAGCAGTTCGCCTACCACCATCTACCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTCGCCTGACGGGCaa > 2:449197/1‑80 (MQ=255)
cGTGCCAGCAGTTCGCCTACCACCATCTACCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTCGCCTGACGGGCaaa < 2:449148/80‑1 (MQ=255)
ccAGCAGTTCGCCTACCACCATCTACCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTCGCCTGACGGGCAAAAGGc < 2:449149/80‑1 (MQ=255)
cagcagTTCGCCTACCACCATCTACCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTCGCCTGACGGGCAAAAGGcgt < 2:449150/81‑1 (MQ=255)
cagcagTTCGCCTACCACCATCTACCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTCGCCTGACGGGCAAAAGGcgt < 2:449151/81‑1 (MQ=255)
agcagTTCGCCTACCACCATCTACCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTCGCCTGACGGGCAAAAGGcgta > 2:449191/1‑81 (MQ=255)
agcagTTCGCCTACCACCATCTACCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTCGCCTGACGGGCAAAAGGcgta > 2:449193/1‑81 (MQ=255)
cagTTCGCCTACCACCATCTACCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTCGCCTGACGGGCAAAAGGcgtacg > 1:449199/1‑81 (MQ=255)
cagTTCGCCTACCACCATCTACCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTCGCCTGACGGGCAAAAGGcgtacg < 1:449152/81‑1 (MQ=255)
cGCCTACCACCATCTACCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTCGCCTGACGGGCAAAAGGCGTACGTAAAc > 1:449190/1‑81 (MQ=255)
gCCTACCACCATCTACCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTCGCCTGACGGGCAAAAGGCGTACGTAAAcc < 1:449153/81‑1 (MQ=255)
gCCTACCACCATCTACCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTCGCCTGACGGGCAAAAGGCGTACGTAAAc < 1:449154/80‑1 (MQ=255)
tACCACCATCTACCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTCGCCTGACGGGCAAAAGGCGTACGTAAACCGc > 2:449192/1‑80 (MQ=255)
caccaTCTACCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTCGCCTGACGGGCAAAAGGCGTACGTAAACCGCTAAc < 1:449155/81‑1 (MQ=255)
tCTACCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTCGCCTGACGGGCAAAAGGCGTACGTAAACCGCTAACAATgg > 1:449194/1‑81 (MQ=255)
|
CGACCTGACCAAAGACCAGTTGTTCAATCACTTCGGCGGGGATCTCGCTGCGTGCCAGCAGTTCGCCTACCACCATCTTCCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTCGCCTGACGGGCAAAAGGCGTACGTAAACCGCTAACAATGG > NC_000913/2460268‑2460423
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
