Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,555,565 |
G→T |
A55E (GCG→GAG) |
eutK ← |
putative structural protein, ethanolamine utilization microcompartment |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,555,565 | 0 | G | T | 100.0%
| 152.6
/ NA
| 43 | A55E (GCG→GAG) | eutK | putative structural protein, ethanolamine utilization microcompartment |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (22/21); total (22/21) |
GTCTGGCCGACCGATCTCCTTGCGGCTGATGACACGCCCGGTACGCATCGCGGCAGCACAACCTGCGTCCAGCGCCGCACGACACGCCGCCAGATCGCCTTCCACCACCAGCGTTAAGCGACCAGGGTCAAGCACTTCGTGACTGAG > NC_000913/2555489‑2555635
|
gTCTGGCCGACCGATCTCCTTGCGGCTGATGACACGCCCGGTACGCATCGCGGCAGCACAACCTGCGTCCAGCGCCTCAc > 1:465841/1‑80 (MQ=255)
gTCTGGCCGACCGATCTCCTTGCGGCTGATGACACGCCCGGTACGCATCGCGGCAGCACAACCTGCGTCCAGCGCCTCAc < 2:465840/80‑1 (MQ=255)
gTCTGGCCGACCGATCTCCTTGCGGCTGATGACACGCCCGGTACGCATCGCGGCAGCACAACCTGCGTCCAGCGCCTCACg < 2:465839/81‑1 (MQ=255)
gCCGACCGATCTCCTTGCGGCTGATGACACGCCCGGTACGCATCGCGGCAGCACAACCTGCGTCCAGCGCCTCACGACAcg < 2:465842/81‑1 (MQ=255)
cgaccgaTCTCCTTGCGGCTGATGACACGCCCGGTACGCATCGCGGCAGCACAACCTGCGTCCAGCGCCTCACGACAcgcc < 2:465843/81‑1 (MQ=255)
gaTCTCCTTGCGGCTGATGACACGCCCGGTACGCATCGCGGCAGCACAACCTGCGTCCAGCGCCTCACGACAcgccgc > 2:465900/1‑78 (MQ=255)
aTCTCCTTGCGGCTGATGACACGCCCGGTACGCATCGCGGCAGCACAACCTGCGTCCAGCGCCTCACGACAcgccgc > 1:465905/1‑77 (MQ=255)
tctcCTTGCGGCTGATGACACGCCCGGTACGCATCGCGGCAGCACAACCTGCGTCCAGCGCCTCACGACAcgccgc > 2:465901/1‑76 (MQ=255)
ctcCTTGCGGCTGATGACACGCCCGGTACGCATCGCGGCAGCACAACCTGCGTCCAGCGCCTCACGACAcgccgc > 2:465895/1‑75 (MQ=255)
cTTGCGGCTGATGACACGCCCGGTACGCATCGCGGCAGCACAACCTGCGTCCAGCGCCTCACGACACGCCGCCAg < 2:465844/75‑1 (MQ=255)
gCGGCTGATGACACGCCCGGTACGCATCGCGGCAGCACAACCTGCGTCCAGCGCCTCACGACACGCCGCCAGATCGCCt < 1:465845/79‑1 (MQ=255)
gCGGCTGATGACACGCCCGGTACGCATCGCGGCAGCACAACCTGCGTCCAGCGCCTCACGACACGCCGCCAGATCGCCt < 2:465846/79‑1 (MQ=255)
cGGCTGATGACACGCCCGGTACGCATCGCGGCAGCACAACCTGCGTCCAGCGCCTCACGACACGCCGCCAGATCGCCTTc < 2:465847/80‑1 (MQ=255)
ggCTGATGACACGCCCGGTACGCATCGCGGCAGCACAACCTGCGTCCAGCGCCTCACGACACGCCGCCAGATCGCCTTcca > 1:465903/1‑81 (MQ=255)
ggCTGATGACACGCCCGGTACGCATCGCGGCAGCACAACCTGCGTCCAGCGCCTCACGACACGCCGCCAGATCGCCTTcca > 1:465922/1‑81 (MQ=255)
tgatgaCACGCCCGGTACGCATCGCGGCAGCACAACCTGCGTCCAGCGCCTCACGACACGCCGCCAGATCGCCTTccacca > 2:465907/1‑81 (MQ=255)
gatgaCACGCCCGGTACGCATCGCGGCAGCACAACCTGCGTCCAGCGCCTCACGACACGCCGCCAGATCGCCTTccaccac > 1:465916/1‑81 (MQ=255)
gaCACGCCCGGTACGCATCGCGGCAGCACAACCTGCGTCCAGCGCCTCACGACACGCCGCCAGATCGCCTTCCACCACCAg > 1:465909/1‑81 (MQ=255)
gCCCGGTACGCATCGCGGCAGCACAACCTGCGTCCAGCGCCTCACGACACGCCGCCAGATCGCCTTCCACCACCAGCGtt > 1:465919/1‑80 (MQ=255)
gCCCGGTACGCATCGCGGCAGCACAACCTGCGTCCAGCGCCTCACGACACGCCGCCAGATCGCCTTCCACCACCAGCGTTa < 1:465848/81‑1 (MQ=255)
cGGTACGCATCGCGGCAGCACAACCTGCGTCCAGCGCCTCACGACACGCCGCCAGATCGCCTTCCACCACCAGCGTTAAGc < 2:465849/81‑1 (MQ=255)
gTACGCATCGCGGCAGCACAACCTGCGTCCAGCGCCTCACGACACGCCGCCAGATCGCCTTCCACCACCAGCGTTAAGCGa > 2:465915/1‑81 (MQ=255)
cGCATCGCGGCAGCACAACCTGCGTCCAGCGCCTCACGACACGCCGCCAGATCGCCTTCCACCACCAGCGTTAAGCGAc > 1:465926/1‑79 (MQ=255)
cGCATCGCGGCAGCACAACCTGCGTCCAGCGCCTCACGACACGCCGCCAGATCGCCTTCCACCACCAGCGTTAAGCGACCa < 1:465850/81‑1 (MQ=255)
gCATCGCGGCAGCACAACCTGCGTCCAGCGCCTCACGACACGCCGCCAGATCGCCTTCCACCACCAGCGTTAAGCGACCAg < 1:465851/81‑1 (MQ=255)
aTCGCGGCAGCACAACCTGCGTCCAGCGCCTCACGACACGCCGCCAGATTGCCTTCCACCACCAGCGTTAAGCGACCAggg > 2:465908/1‑81 (MQ=255)
aTCGCGGCAGCACAACCTGCGTCCAGCGCCTCACGACACGCCGCCAGATCGCCTTCCACCACCAGCGTTAAGCGACCAggg > 2:465917/1‑81 (MQ=255)
tCGCGGCAGCACAACCTGCGTCCAGCGCCTCACGACACGCCGCCAGATCGCCTTCCACCACCAGCGTTAAGCGACCAGGGt < 2:465852/81‑1 (MQ=255)
cgGCAGCACAACCGGCGTCCAGCGCCTCACGACACGCCGCCAGATCGCCTTCCACCACCAGCGTTAAGCGACCAGGGTCaa < 1:465853/81‑1 (MQ=255)
ggCAGCACAACCTGCGTCCAGCGCCTCACGACACGCCGCCAGATCGCCTTCCACCACCAGCGTTAAGCGACCAGGGTCAAg > 2:465902/1‑81 (MQ=255)
gcagcaCAACCTGCGTCCAGCGCCTCACGACACGCCGCCAGATCGCCTTCCACCACCAGCGTTAAGCGACCAGGGTCAAg > 2:465920/1‑80 (MQ=255)
agcaCAACCTGCGTCCAGCGCCTCACGACACGCCGCCAGATCGCCTTCCACCACCAGCGTTAAGCGACCAGGGTCAAGCAc > 1:465930/1‑81 (MQ=255)
agcaCAACCTGCGTCCAGCGCCTCACGACACGCCGCCAGATCGCCTTCCACCACCAGCGTTAAGCGACCAGGGTCAAGCAc > 2:465899/1‑81 (MQ=255)
agcaCAACCTGCGTCCAGCGCCTCACGACACGCCGCCAGATCGCCTTCCACCACCAGCGTTAAGCGACCAGGGTCAAGCAc < 1:465854/81‑1 (MQ=255)
gcaCAACCTGCGTCCAGCGCCTCACGACACGCCGCCAGATCGCCTTCCACCACCAGCGTTAAGCGACCAGGGTCAAGCACt < 1:465855/81‑1 (MQ=255)
acaACCTGCGTCCAGCGCCTCACGACACGCCGCCAGATCGCCTTCCACCACCAGCGTTAAGCGACCAGGGTCAAGCACTTc < 2:465856/81‑1 (MQ=255)
ccTGCGTCCAGCGCCTCACGACACGCCGCCAGATCGCCTTCCACCACCAGCGTTAAGCGACCAGGGTCAAGCACTTCGTGa < 1:465857/81‑1 (MQ=255)
cTGCGTCCAGCGCCTCACGACACGCCGCCAGATCGCCTTCCACCACCAGCGTTAAGCGACCAGGGTCAAGCACTTCGTGAc < 1:465858/81‑1 (MQ=255)
cGTCCAGCGCCTCACGACACGCCGCCAGATCGCCTTCCACCACCAGCGTTAAGCGACCAGGGTCAAGCACTTCGTGACTg < 2:465859/80‑1 (MQ=255)
cGTCCAGCGCCTCACGACACGCCGCCAGATCGCCTTCCACCACCAGCGTTAAGCGACCAGGGTCAAGCACTTCGTGACTg < 1:465860/80‑1 (MQ=255)
gTCCAGCGCCTCACGACACGCCGCCAGATCGCCTTCCACCACCAGCGTTAAGCGACCAGGGTCAAGCACTTCGTGACTGAg > 1:465913/1‑81 (MQ=255)
gTCCAGCGCCTCACGACACGCCGCCAGATCGCCTTCCACCACCAGCGTTAAGCGACCAGGGTCAAGCACTTCGTGACTGAg > 2:465911/1‑81 (MQ=255)
ccAGCGCCTCACGACACGCCGCCAGATCGCCTTCCACCACCAGCGTTAAGCGACCAGGGTCAAGCACTTCGTGACTGAg > 1:465932/1‑79 (MQ=255)
|
GTCTGGCCGACCGATCTCCTTGCGGCTGATGACACGCCCGGTACGCATCGCGGCAGCACAACCTGCGTCCAGCGCCGCACGACACGCCGCCAGATCGCCTTCCACCACCAGCGTTAAGCGACCAGGGTCAAGCACTTCGTGACTGAG > NC_000913/2555489‑2555635
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
