Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,860,308 |
T→C |
F183F (TTT→TTC) |
pphB → |
phosphoprotein phosphatase 2 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,860,308 | 0 | T | C | 100.0%
| 63.7
/ NA
| 19 | F183F (TTT→TTC) | pphB | phosphoprotein phosphatase 2 |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (13/6); total (13/6) |
TTACTCTGGCCTGTTGATCGTGTGCAGAAATCGCTTAATGGCGAGTTACAACAAATAAACGGCGCTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGC > NC_000913/2860234‑2860378
|
ttACTCTGGCCTGTTGATCGTGTGCAGAAATCGCTTAATGGCGAGTTACAACAAATAAACGGCGCTGATTATTTCATAttt < 2:528380/81‑1 (MQ=255)
aCTCTGGCCTGTTGATCGTGTGCAGAAATCGCTTAATGGCGAGTTACAACAAATAAACGGCGCTGATTATTTCATATTTg > 2:528403/1‑80 (MQ=255)
ctctGGCCTGTTGATCGTGTGCAGAAATCGCTTAATGGCGAGTTACAACAAATAAACGGCGCTGATTATTTCATATTTGGa > 1:528414/1‑81 (MQ=255)
ctGGCCTGTTGATCGTGTGCAGAAATCGCTTAATGGCGAGTTACAACAAATAAACGGCGCTGATTATTTCATATTTGGACa > 1:528409/1‑81 (MQ=255)
cGTGTGCAGAAATCGCTTAATGGCGAGTTACAACAAATAAACGGCGCTGATTATTTCATATTTGGACATATGATGTTTGa > 1:528415/1‑80 (MQ=255)
tgtgCAGAAATCGCTTAATGGCGAGTTACAACAAATAAACGGCGCTGATTATTTCATATTTGGACATATGATGTTTGATaa > 1:528407/1‑81 (MQ=255)
tAATGGCGAGTTACAACAAATAAACGGCGCTGATTATTTCATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGc > 1:528420/1‑81 (MQ=255)
tAATGGCGAGTTACAACAAATAAACGGCGCTGATTATTTCATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGc > 1:528408/1‑81 (MQ=255)
aaTGGCGAGTTACAACAAATAAACGGCGCTGATTATTTCATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGc > 2:528416/1‑80 (MQ=255)
aTGGCGAGTTACAACAAATAAACGGCGCTGATTATTTCATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCt > 2:528406/1‑80 (MQ=255)
cGAGTTACAACAAATAAACGGCGCTGATTATTTCATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAAc > 1:528417/1‑79 (MQ=255)
gAGTTACAACAAATAAACGGCGCTGATTATTTCATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAAc > 2:528381/1‑78 (MQ=255)
acaacaAATAAACGGCGCTGATTATTTCATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACTAGATTta > 2:528413/1‑81 (MQ=255)
acaacaAATAAACGGCGCTGATTATTTCATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGAttt < 2:528382/80‑1 (MQ=255)
caaATAAACGGCGCTGATTATTTCATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATAtt < 2:528383/81‑1 (MQ=255)
aCGGCGCTGATTATTTCATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCg < 2:528384/81‑1 (MQ=255)
cgcTGATTATTTCATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATc < 2:528385/81‑1 (MQ=255)
cgcTGATTATTTCATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATc < 1:528386/81‑1 (MQ=255)
cTGATTATTTCATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGc > 1:528411/1‑81 (MQ=255)
|
TTACTCTGGCCTGTTGATCGTGTGCAGAAATCGCTTAATGGCGAGTTACAACAAATAAACGGCGCTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGC > NC_000913/2860234‑2860378
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
