Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,932,902 |
C→A |
D38Y (GAT→TAT) |
fucO ← |
L‑1,2‑propanediol oxidoreductase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,932,902 | 0 | C | A | 100.0%
| 79.8
/ NA
| 23 | D38Y (GAT→TAT) | fucO | L‑1,2‑propanediol oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (12/11); total (12/11) |
AATCGCCCATGCCAGCCCTGCAGCATCCATCTTATCGGTCACTTTCGCCACCACGCCGCATTGCACCAGCGTTTTATCGGTGACGATCAGCGCCTTCTGATAACCACGGCGTTTCACCTCATCGGTTAAAGCCCCAACAGCACCCCGACCAAAC > NC_000913/2932825‑2932978
|
aaTCGCCCATGCCAGCCCTGCAGCATCCATCTTATCGGTCACTTTCGCCACCACGCCGCATTGCACCAGCGTTTTATAgg < 1:543042/80‑1 (MQ=255)
aaTCGCCCATGCCAGCCCTGCAGCATCCATCTTATCGGTCACTTTCGCCACCACGCCGCATTGCACCAGCGTTTTATAgg < 2:543043/80‑1 (MQ=255)
aaTCGCCCATGCCAGCCCTGCAGCATCCATCTTATCGGTCACTTTCGCCACCACGCCGCATTGCACCAGCGTTTTATAGGt > 1:543084/1‑81 (MQ=255)
gCCCATGCCAGCCCTGCAGCATCCATCTTATCGGTCACTTTCGCCACCACGCCGCATTGCACCAGCGTTTTATAGGTGAc < 1:543044/80‑1 (MQ=255)
gCCCATGCCAGCCCTGCAGCATCCATCTTATCGGTCACTTTCGCCACCACGCCGCATTGCACCAGCGTTTTATAGGTGAc > 1:543095/1‑80 (MQ=255)
ccAGCCCTGCAGCATCCATCTTATCGGTCACTTTCGCCACCACGCCGCATTGCACCAGCGTTTTATAGGTGACGATCAgc < 1:543045/80‑1 (MQ=255)
cAGCCCTGCAGCATCCATCTTATCGGTCACTTTCGCCACCACGCCGCATTGCACCAGCGTTTTATAGGTGACGATCAgcgc > 1:543085/1‑81 (MQ=255)
cccTGCAGCATCCATCTTATCGGTCACTTTCGCCACCACGCCGCATTGCACCAGCGTTTTATAGGTGACGATCAGCGCCtt < 2:543046/81‑1 (MQ=255)
cagcaTCCATCTTATCGGTCACTTTCGCCACCACGCCGCATTGCACCAGCGTTTTATAGGTGACGATCAGCGCCTTCTGa > 1:543091/1‑80 (MQ=255)
cagcaTCCATCTTATCGGTCACTTTCGCCACCACGCCGCATTGCACCAGCGTTTTATAGGTGACGATCAGCGCCTTCTGa > 2:681625/1‑80 (MQ=255)
ccatcTTATCGGTCACTTTCGCCACCACGCCGCATTGCACCAGCGTTTTATAGGTGACGATCAGCGCCTTCTGATAACCAc > 2:543086/1‑81 (MQ=255)
cTTATCGGTCACTTTCGCCACCACGCCGCATTGCACCAGCGTTTTATAGGTGACGATCAGCGCCTTCTGATAACCACGGCg > 2:543088/1‑81 (MQ=255)
ggTCACTTTCGCCACCACGCCGCATTGCACCAGCGTTTTATAGGTGACGATCAGCGCCTTCTGATAACCACGGCGTTTCAc < 2:543047/81‑1 (MQ=255)
ccaccaCGCCGCATTGCACCAGCGTTTTATAGGTGACGATCAGCGCCTTCTGATAACCACGGCGTTTCACCTCATCGGtt > 1:543090/1‑80 (MQ=255)
ccaccaCGCCGCATTGCACCAGCGTTTTATAGGTGACGATCAGCGCCTTCTGATAACCACGGCGTTTCACCTCATCGGTTa < 2:543048/81‑1 (MQ=255)
caccacGCCGCATTGCACCAGCGTTTTATAGGTGACGATCAGCGCCTTCTGATAACCACGGCGTTTCACCTCATCGGTTa > 2:543096/1‑80 (MQ=255)
cacGCCGCATTGCACCAGCGTTTTATAGGTGACGATCAGCGCCTTCTGATAACCACGGCGTTTCACCTCATCGGTTAAAGc > 2:543097/1‑81 (MQ=255)
acGCCGCATTGCACCAGCGTTTTATAGGTGACGATCAGCGCCTTCTGATAACCACGGCGTTTCACCTCATCGGTTAAAGcc < 2:543049/81‑1 (MQ=255)
aCCAGCGTTTTATAGGTGACGATCAGCGCCTTCTGATAACCACGGCGTTTCACCTCATCGGTTAAAGCCCCAACAGCAccc < 2:543050/81‑1 (MQ=255)
cAGCGTTTTATAGGTGACGATCAGCGCCTTCTGATAACCACGGCGTTTCACCTCATCGGTTAAAGCCCCAACAGCAcccc > 2:543092/1‑80 (MQ=255)
cGTTTTATAGGTGACGATCAGCGCCTTCTGATAACCACGGCGTTTCACCTCATCGGTTAAAGCCCCAACAGCACCCCGAcc < 2:543051/81‑1 (MQ=255)
gTTTTATAGGTGACGATCAGCGCCTTCTGATAACCACGGCGTTTCACCTCATCGGTTAAAGCCCCAACAGCACCCCGACCa < 1:543052/81‑1 (MQ=255)
ttATAGGTGACGATCAGCGCCTTCTGATAACCACGGCGTTTCACCTCATCGGTTAAAGCCCCAACAGCACCCCGACCAAAc > 1:543093/1‑81 (MQ=255)
|
AATCGCCCATGCCAGCCCTGCAGCATCCATCTTATCGGTCACTTTCGCCACCACGCCGCATTGCACCAGCGTTTTATCGGTGACGATCAGCGCCTTCTGATAACCACGGCGTTTCACCTCATCGGTTAAAGCCCCAACAGCACCCCGACCAAAC > NC_000913/2932825‑2932978
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
