Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,672,739 |
Δ1 bp |
coding (326/900 nt) |
yhjC → |
putative DNA‑binding transcriptional regulator YhjC |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,672,738 | 0 | C | . | 82.9%
| 93.1
/ 28.8
| 35 | coding (325/900 nt) | yhjC | putative DNA‑binding transcriptional regulator YhjC |
| Reads supporting (aligned to +/- strand): ref base C (3/3); new base . (15/14); total (18/17) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.28e-01 |
GTTTCAACAGGATGCCACCAGTATCAGCGGTAAATTACGCATCGACATCCCGCCAGGAATCGCGAAAAGCCTGTTACTGCCGCGCCTGTCGGAATTTCTCTATCTGCATCCGGGAATTGAGCTGGAACTGAGTAGCCATGACCGTCCGGTAGATATTC > NC_000913/3672659‑3672816
|
gTTTCAACAGGATGCCACCAGTATCAGCGGTAAATTACGCATCGACATCCCGCCAGGAATCGCGAAAAGCCTGTTACTGcg > 1:709351/1‑80 (MQ=255)
tttCAACAGGATGCCACCAGTATCAGCGGTAAATTACGCATCGACATCCCGCCAGGAATCGCGAAAAGCCTGTTACTGcgc < 1:709315/81‑3 (MQ=255)
cAACAGGATGCCACCAGTATCAGCGGTAAATTACGCATCGACATCCCGCCAGGAATCGCGAAAAGCCTGTTACTG‑CGCGcc > 2:709350/1‑81 (MQ=255)
aCAGGATGCCACCAGTATCAGCGGTAAATTACGCATCGACATCCCGCCAGGAATCGCGAAAAGCCTGTTACTG‑CGCGCCTg < 2:709316/81‑1 (MQ=255)
cAGGATGCCACCAGTATCAGCGGTAAATTACGCATCGACATCCCGCCAGGAATCGCGAAAAGCCTGTTACTG‑CGCGCCTGt > 2:709352/1‑81 (MQ=255)
aGGATGCCACCAGTATCAGCGGTAAATTACGCATCGACATCCCGCCAGGAATCGCGAAAAGCCTGTTACTGCCGCGCCTGt < 1:709317/81‑1 (MQ=255)
aGGATGCCACCAGTATCAGCGGTAAATTACGCATCGACATCCCGCCAGGAATCGCGAAAAGCCTGTTACTG‑CGCGCCTGTc < 2:709318/81‑1 (MQ=255)
tGCCACCAGTATCAGCGGTAAATTACGCATCGACATCCCGCCAGGAATCGCGAAAAGCCTGTTACTG‑CGCGCCTGTCGGaa < 2:709320/81‑1 (MQ=255)
tGCCACCAGTATCAGCGGTAAATTACGCATCGACATCCCGCCAGGAATCGCGAAAAGCCTGTTACTG‑CGCGCCTGTCGGaa < 1:709319/81‑1 (MQ=255)
ccaccaGTATCAGCGGTAAATTACGCATCGACATCCCGCCAGGAATCGCGAAAAGCCTGTTACTG‑CGCGCCTGTCGGAAtt < 2:709321/81‑1 (MQ=255)
ccaccaGTATCAGCGGTAAATTACGCATCGACATCCCGCCAGGAATCGCGAAAAGCCTGTTACTG‑CGCGCCTGTCGGAAtt > 2:709369/1‑81 (MQ=255)
caccaGTATCAGCGGTAAATTACGCATCGACATCCCGCCAGGAATCGCGAAAAGCCTGTTACTG‑CGCGCCTGTCGGAAttt < 2:709322/81‑1 (MQ=255)
gTATCAGCGGTAAATTACGCATCGACATCCCGCCAGGAATCGCGAAAAGCCTGTTACTGCCGCGCCTGTCGGAATTTctct < 1:709323/81‑1 (MQ=255)
gTATCAGCGGTAAATTACGCATCGACATCCCGCCAGGAATCGCGAAAAGCCTGTTACTG‑CGCGCCTGTCGGAATTTCTCTa > 2:709364/1‑81 (MQ=255)
aTCAGCGGTAAATTACGCATCGACATCCCGCCAGGAATCGCGAAAAGCCTGTTACTGCCGCGCCTGTCGGAATTTCTCTAt > 1:709354/1‑81 (MQ=255)
aTCAGCGGTAAATTACGCATCGACATCCCGCCAGGAATCGCGAAAAGCCTGTTACTG‑CGCGCCTGTCGGAATTTCTCTATc > 2:709380/1‑81 (MQ=255)
tCAGCGGTAAATTACGCATCGACATCCCGCCAGGAATCGCGAAAAGCCTGTTACTG‑CGCGCCTGTCGGAATTTCTCTATc < 1:709324/80‑1 (MQ=255)
tCAGCGGTAAATTACGCATCGACATCCCGCCAGGAATCGCGAAAAGCCTGTTACTG‑CGCGCCTGTCGGAATTTCTCTATCt < 2:709325/81‑1 (MQ=255)
gCGGTAAATTACGCATCGACATCCCGCCAGGAATCGCGAAAAGCCTGTTACTG‑CGCGCCTGTCGGAATTTCTCTATCTGCa > 1:709384/1‑81 (MQ=255)
aTTACGCATCGACATCCCGCCAGGAATCGCGAAAAGCCTGTTACTG‑CGCGCCTGTCGGAATTTCTCTATCTGCATCCggg > 2:709368/1‑80 (MQ=255)
aTTACGCATCGACATCCCGCCAGGAATCGCGAAAAGCCTGTTACTG‑CGCGCCTGTCGGAATTTCTCTATCTGCATCCGGGa < 2:709326/81‑1 (MQ=255)
cGCATCGACATCCCGCCAGGAATCGCGAAAAGCCTGTTACTG‑CGCGCCTGTCGGAATTTCTCTATCTGCATCCGGGAAtt < 2:709327/80‑1 (MQ=255)
gCATCGACATCCCGCCAGGAATCGCGAAAAGCCTGTTACTG‑CGCGCCTGTCGGAATTTCTCTATCTGCATCCGGGAAtt > 1:709375/1‑79 (MQ=255)
gCATCGACATCCCGCCAGGAATCGCGAAAAGCCTGTTACTG‑CGCGCCTGTCGGAATTTCTCTATCTGCATCCGGGAAtt > 1:709370/1‑79 (MQ=255)
cGACATCCCGCCAGGAATCGCGAAAAGCCTGTTACTG‑CTCGCCTGTCGGAATTTCTCTATCTGCATCCGGGAATTGAGCTg > 2:709381/1‑81 (MQ=255)
cGACATCCCGCCAGGAATCGCGAAAAGCCTGTTACTG‑CGCGCCTGTCGGAATTTCTCTATCTGCATCCGGGAATTGAGCTg > 2:709373/1‑81 (MQ=255)
gACATCCCGCCAGGAATCGCGAAAAGCCTGTTACTG‑CGCGCCTGTCGGAATTTCTCTATCTGCATCCGGGAATTGAGCTgg < 1:709328/81‑1 (MQ=255)
cctccAGGAATCGCGAAAAGCCTGTTACTG‑CGCGCCTGTCGGAATTTCTCTATCTGCATCCGGGAATTGAGCTGTAACt > 2:709362/4‑79 (MQ=255)
ggAATCGCGAAAAGCCTGTTACTG‑CGCGCCTGTCGGAATTTCTCTATCTGCATCCGGGAATTGAGCTGGAACTGAGTAGc > 1:709390/1‑80 (MQ=255)
gAATCGCGAAAAGCCTGTTACTG‑CGCGCCTGTCGGAATTTCTCTATCTGCATCCGGGAATTGAGCTGGAACTGAGTAGCCa < 1:709329/81‑1 (MQ=255)
aTCGCGAAAAGCCTGTTACTG‑CGCGCCTGTCGGAATTTCTCTATCTGCATCCGGGAATTGAGCTGGAACTGAGTAGCCAt > 2:709377/1‑80 (MQ=255)
gAAAAGCCTGTTACTG‑CGCGCCTGTCGGAATTTCTCTATCTGCATCCGGGAATTGAGCTGGAACTGAGTAGCCATGACCGt < 1:709330/81‑1 (MQ=255)
aaaGCCTGTTACTGCCGCGCCTGTCGGAATTTCTCTATCTGCATCCGGGAATTGAGCTGGAACTGAGTAGCCATGACCGTc > 1:709379/1‑81 (MQ=255)
aaGCCTGTTACTGCCGCGCCTGTCGGAATTTCTCTATCTGCATCCGGGAATTGAGCTGGAACTGAGTAGCCATGACCGTcc < 2:709331/81‑1 (MQ=255)
gCCTGTTACTG‑CGCGCCTGTCGGAATTTCTCTATCTGCATCCGGGAATTGAGCTGGAACTGAGTAGCCATGACCGTCCGGt < 1:709332/81‑1 (MQ=255)
tGTTACTG‑CGCGCCTGTCGGAATTTCTCTATCTGCATCCGGGAATTGAGCTGGAACTGAGTAGCCATGACCGTCCgg > 2:709372/1‑77 (MQ=255)
tGCCGCGCCTGTCGGAATTTCTCTATCTGCATCCGGGAATTGAGCTGGAACTGAGTAGCCATGACCGTCCGGTAGATAttc > 2:709376/1‑81 (MQ=255)
|
GTTTCAACAGGATGCCACCAGTATCAGCGGTAAATTACGCATCGACATCCCGCCAGGAATCGCGAAAAGCCTGTTACTGCCGCGCCTGTCGGAATTTCTCTATCTGCATCCGGGAATTGAGCTGGAACTGAGTAGCCATGACCGTCCGGTAGATATTC > NC_000913/3672659‑3672816
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
