Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,734,789 |
T→A |
L357Q (CTG→CAG) |
xylH → |
xylose ABC transporter membrane subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,734,789 | 0 | T | A | 100.0%
| 112.5
/ NA
| 31 | L357Q (CTG→CAG) | xylH | xylose ABC transporter membrane subunit |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (16/15); total (16/15) |
TTGGCGGCACCAGCCTGGCTGGCGGTGTGGGAAGCGTTGCCGGAGCAGTAATGGGGGCATTTATCATGGCTTCACTGGATAACGGCATGAGTATGATGGATGTACCGACCTTCTGGCAGTATATCGTTAAAGGTGCGATTCTGTTGCTG > NC_000913/3734714‑3734862
|
ttGGCGGCACCAGCCTGGCTGGCGGTGTGGGAAGCGTTGCCGGAGCAGTAATGGGGGCATTTATCATGGCTTCACAGGAt > 1:724147/1‑80 (MQ=255)
ttGGCGGCACCAGCCTGGCTGGCGGTGTGGGAAGCGTTGCCGGAGCAGTAATGGGGGCATTTATCATGGCTTCACAGGATa < 1:724091/81‑1 (MQ=255)
ggcggcACCAGCCTGGCTGGCGGTGTGGGAAGCGTTGCCGGAGCAGTAATGGGGGCATTTATCATGGCTTCACAGGATAAc > 1:724137/1‑81 (MQ=255)
gcggcACCAGCCTGGCTGGCGGTGTGGGAAGCGTTGCCGGAGCAGTAATGGGGGCATTTATCATGGCTTCACAGGATAAc < 1:724092/80‑1 (MQ=255)
gcggcACCAGCCTGGCTGGCGGTGTGGGAAGCGTTGCCGGAGCAGTAATGGGGGCATTTATCATGGCTTCACAGGATAACg < 1:724093/81‑1 (MQ=255)
aCCAGCCTGGCTGGCGGTGTGGGAAGCGTTGCCGGAGCAGTAATGGGGGCATTTATCATGGCTTCACAGGATAACGGCATg < 1:724094/81‑1 (MQ=255)
aGCCTGGCTGGCGGTGTGGGAAGCGTTGCCGGAGCAGTAATGGGGGCATTTATCATGGCTTCACAGGATAACGGCATGAGt < 1:724095/81‑1 (MQ=255)
gCCTGGCTGGCGGTGTGGGAAGCGTTGCCGGAGCAGTAATGGGGGCATTTATCATGGCTTCACAGGATAACGGCATGAGTa > 2:724131/1‑81 (MQ=255)
ccTGGCTGGCGGTGTGGGAAGCGTTGCCGGAGCAGTAATGGGGGCATTTATCATGGCTTCACAGGATAACGGCATGAGTat > 2:724139/1‑81 (MQ=255)
gctggcGGTGTGGGAAGCGTTGCCGGAGCAGTAATGGGGGCATTTATCATGGCTTCACAGGATAACGGCATGAGTatgat < 2:724096/80‑1 (MQ=255)
gcGGTGTGGGAAGCGTTGCCGGAGCAGTAATGGGGGCATTTATCATGGCTTCACAGGATAACGGCATGAGTATgatggatg > 2:724151/1‑81 (MQ=255)
gcGGTGTGGGAAGCGTTGCCGGAGCAGTAATGGGGGCATTTATCATGGCTTCACAGGATAACGGCATGAGTATgatggatg > 1:724143/1‑81 (MQ=255)
cGGTGTGGGAAGCGTTGCCGGAGCAGTAATGGGGGCATTTATCATGGCTTCACAGGATAACGGCATGAGTATgatggatg > 1:724149/1‑80 (MQ=255)
cGGTGTGGGAAGCGTTGCCGGAGCAGTAATGGGGGCATTTATCATGGCTTCACAGGATAACGGCATGAGTATGATGGATGt < 1:724097/81‑1 (MQ=255)
gtgtGGGAAGCGTTGCCGGAGCAGTAATGGGGGCATTTATCATGGCTTCACAGGATAACGGCATGAGTATGATGGATGTAc < 2:724098/81‑1 (MQ=255)
ggAAGCGTTGCCGGAGCAGTAATGGGGGCATTTATCATGGCTTCACAGGATAACGGCATGAGTATGATGGATGTACCGAcc > 2:724138/1‑81 (MQ=255)
aGCGTTGCCGGAGCAGTAATGGGGGCATTTATCATGGCTTCACAGGATAACGGCATGAGTATGATGGATGTACCGACCTTc > 1:724140/1‑81 (MQ=255)
gCGTTGCCGGAGCAGTAATGGGGGCATTTATCATGGCTTCACAGGATAACGGCATGAGTATGATGGATGTACCGACCTTCt < 2:724100/81‑1 (MQ=255)
gCGTTGCCGGAGCAGTAATGGGGGCATTTATCATGGCTTCACAGGATAACGGCATGAGTATGATGGATGTACCGACCTTCt < 2:724099/81‑1 (MQ=255)
gTTGCCGGAGCAGTAATGGGGGCATTTATCATGGCTTCACAGGATAACGGCATGAGTATGATGGATGTACCGACCTTCTgg > 1:724142/1‑81 (MQ=255)
ttGCCGGAGCAGTAATGGGGGCATTTATCATGGCTTCACAGGATAACGGCATGAGTATGATGGATGTACCGACCTTCTgg > 1:724146/1‑80 (MQ=255)
ttGCCGGAGCAGTAATGGGGGCATTTATCATGGCTTCACAGGATAACGGCATGAGTATGATGGATGTACCGACCTTCTGGc < 1:724101/81‑1 (MQ=255)
gCCGGAGCAGTAATGGGGGCATTTATCATGGCTTCACAGGATAACGGCATGAGTATGATGGATGTACCGACCTTCTGGCa < 1:724102/80‑1 (MQ=255)
gAGCAGTAATGGGGGCATTTATCATGGCTTCACAGGATAACGGCATGAGTATGATGGATGTACCGACCTTCTGGCAGtata > 1:724141/1‑81 (MQ=255)
aaTGGGGGCATTTATCATGGCTTCACAGGATAACGGCATGAGTATGATGGATGTACCGACCTTCTGGCAGTATATCGTTa > 1:724150/1‑80 (MQ=255)
aTGGGGGCATTTATCATGGCTTCACAGGATAACGGCATGAGTATGATGGATGTACCGACCTTCTGGCAGTATATCGTTaa < 1:724103/80‑1 (MQ=255)
aTTTATCATGGCTTCACAGGATAACGGCATGAGTATGATGGATGTACCGACCTCCTGGCAGTATATCGTTAAAGGTGCGAt > 1:724148/1‑81 (MQ=255)
tttATCATGGCTTCACAGGATAACGGCATGAGTATGATGGATGTACCGACCTTCTGGCAGTATATCGTTAAAGGTGCGAtt > 1:724152/1‑81 (MQ=255)
aTCATGGCTTCACAGGATAACGGCATGAGTATGATGGATGTACCGACCTTCTGGCAGTATATCGTTAAAGGTGCGATTCTg > 2:724161/1‑81 (MQ=255)
tCATGGCTTCACAGGATAACGGCATGAGTATGATGGATGTACCGACCTTCTGGCAGTATATCGTTAAAGGTGCGATTCTGt < 2:724104/81‑1 (MQ=255)
gCTTCACAGGATAACGGCATGAGTATGATGGATGTACCGACCTTCTGGCAGTATATCGTTAAAGGTGCGATTCTGTTGCTg < 1:724105/81‑1 (MQ=255)
|
TTGGCGGCACCAGCCTGGCTGGCGGTGTGGGAAGCGTTGCCGGAGCAGTAATGGGGGCATTTATCATGGCTTCACTGGATAACGGCATGAGTATGATGGATGTACCGACCTTCTGGCAGTATATCGTTAAAGGTGCGATTCTGTTGCTG > NC_000913/3734714‑3734862
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
