Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F23 I0 R1 29 39.7 1391800 89.8% 1249836 148.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913_3_hsa_tpiA 2,976,684 G→T 9.6% S29I (AGC→ATC)  galR → galactose‑inducible d‑galactose regulon transcriptional repressor; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_tpiA2,976,6840GT 9.6% 127.9 / 3.0 49S29I (AGC→ATC) galRgalactose‑inducible d‑galactose regulon transcriptional repressor; autorepressor
Reads supporting (aligned to +/- strand):  ref base G (24/20);  new base T (2/3);  total (26/23)
Fisher's exact test for biased strand distribution p-value = 6.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

GAACCAACGGGCGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTTTCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGC‑TGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGTG  >  NC_000913_3_hsa_tpiA/2976536‑2976832
                                                                                                                                                    |                                                                                                                                                     
gAACCAACGGGCGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCCTGGCCACCATAAAGGATGTAGCCCGACTGACAGGCGTTGCAGTCGCCACCCTTTCCCGCGTCATTAATAATTCACCCAAAGCCAg                                                                                                                                                       >  2:359976/1‑149 (MQ=255)
gAACCAACGGGCGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTTGCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAg                                                                                                                                                       <  2:31017/149‑1 (MQ=255)
 aaCCAACGGGCGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTTGCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGc                                                                                                                                                      <  1:140578/149‑1 (MQ=255)
    cAACGGGCGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGACCGACTGGCAAGCGTATCAGTAGCCGCCCTGTCCCCCGACCTTAATAATTACCCCCAACCCCGCGac                                                                                                                                                   >  2:73912/1‑148 (MQ=255)
               ttCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTTGCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCggct                                                                                                                                        <  1:168981/149‑1 (MQ=255)
                    tAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTTGCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGgctggctg                                                                                                                                   >  1:684467/1‑149 (MQ=255)
                      acacTGAAAGAATGTAAGCGTTTAACCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAAGCGTTGCAGTCGCTACCGTTGCTCGCGTCATTAATAATTCACCCACAGCCAGCGCAGCTTCCCggctgggcgta                                                                                                                                 >  2:422765/1‑144 (MQ=255)
                        accgAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATCGCGACCATAAACGATGTACCCCGACGCGCAGTCGTCTCAGTCGCCACCGTCTCCCGCAGCAATAATAATTCACTCACAGCCATCGAACCTTCCCGCCTGCCTGTGCa                                                                                                                               >  2:316933/4‑149 (MQ=255)
                          tGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTTTCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATa                                                                                                                             >  2:376242/1‑149 (MQ=255)
                              aGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTTGCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGc                                                                                                                         >  1:562823/1‑149 (MQ=255)
                                  tGTAAGCCTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGACAGACGTTTCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCATCGAAGCTTCCTGGCTGGCTGTGCATAGTGCAATg                                                                                                                     <  2:641654/149‑1 (MQ=255)
                                    tAAGTGGTTACCCCCTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTTGCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGa                                                                                                                   <  2:625308/149‑1 (MQ=255)
                                    tAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATACCGGATGTAGCCCGACTGGCAGGCGTTGCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGa                                                                                                                   <  1:650990/149‑1 (MQ=255)
                                    tAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTTGCAGTCGCCCCCGTTTCCCGCGTCATTCATCATTGACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATCGTGGCATGGa                                                                                                                   >  1:65344/1‑149 (MQ=255)
                                    tAAGCGTTTACCCACTAAGGTATTTGCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTTGCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGa                                                                                                                   <  1:425072/149‑1 (MQ=255)
                                     aaGCGTTTACCGACTAAGGTATTCTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTTTCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAg                                                                                                                  <  1:375110/149‑1 (MQ=255)
                                      aGCGTTTACCCACTGAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTTGCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGt                                                                                                                 <  1:97515/149‑1 (MQ=255)
                                      aGCGTGTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTTGCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGt                                                                                                                 <  1:19689/149‑1 (MQ=255)
                                          tttGCCCACGAGGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTTGCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTctct                                                                                                             <  2:145410/149‑1 (MQ=255)
                                          tttACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTTGCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTctct                                                                                                             >  2:415896/1‑149 (MQ=255)
                                          tttACCCACTAAGGTATTTTCATGGAGACCATAAAGGATGTAGCCCGACTGGCAGGCGTTGCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTctct                                                                                                             >  1:528168/1‑149 (MQ=255)
                                             aCCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTTGCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCACAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCGATGGAGTCTCTTAg                                                                                                          >  1:123532/1‑149 (MQ=255)
                                             aCCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTTGCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAg                                                                                                          <  1:437365/149‑1 (MQ=255)
                                               ccACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTTGCAGTTGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCt                                                                                                        >  1:446614/1‑149 (MQ=255)
                                                     aGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTTGCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCAcc                                                                                                  <  2:177195/149‑1 (MQ=255)
                                                        tATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTTGCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGa                                                                                               >  1:591021/1‑149 (MQ=255)
                                                         aTTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTTTCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCATCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGaa                                                                                              <  1:270650/149‑1 (MQ=255)
                                                               aTGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTTGCAGTCGCCACCGTTCCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGaacgccaa                                                                                        <  1:108712/149‑1 (MQ=255)
                                                                         tAAAGGATGTAGCCCGACTGGCAGGCGTTGCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTgcg                                                                              >  1:652415/1‑149 (MQ=255)
                                                                         tAAAGGATGTAGCCCGACTGGCAGGCGTTGCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACACCAACGCCCGTgcg                                                                              >  1:623827/1‑149 (MQ=255)
                                                                                   aGCCCGACTGGCAGGCGTTGCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGC‑TGGCgcagc                                                                   <  1:432419/149‑1 (MQ=255)
                                                                                      ccGACTGGCAGGCGTTGCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGC‑TGGCGCAGCAGa                                                                <  1:196832/149‑1 (MQ=255)
                                                                                       cGACTGGCAGGCGTTGCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTCCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGC‑TGGCGCAGCAGAc                                                               <  2:684467/149‑1 (MQ=255)
                                                                                           tGGCAGGCGTTGCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGC‑TGGCGCAGCAGACCACt                                                           >  1:260884/1‑149 (MQ=255)
                                                                                             gCAGGCGTTGCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTGGCTATCACCCGAACGCCAACGCCCGTGCGC‑TGGCGCAGCAGACCACTGa                                                         >  1:184028/1‑149 (MQ=255)
                                                                                             gCAGGCGTTGCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGC‑TGGCGCAGCAGACCACTGa                                                         >  1:298757/1‑149 (MQ=255)
                                                                                                ggCGTTGCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGC‑CGGCGCCGCAGACCACTGAAAc                                                      >  2:186534/1‑149 (MQ=255)
                                                                                                 gCGTTGCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGC‑TGGCGCAGCAGACCACTGAAAcg                                                     >  2:602784/1‑149 (MQ=255)
                                                                                                       cAGTAGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGATGGCTGTGCATAGTGCAATGAAGTCTCTTAGCTATCACCCGCACGCCAACGCCCGT‑CGCATGGCGCAGAAGCCCACTGGAAcggtcggt                                               >  2:25651/1‑149 (MQ=255)
                                                                                                        aGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGC‑TGGCGCAGCAGACCACTGAAACggtcggtc                                              >  2:125147/1‑149 (MQ=255)
                                                                                                         gTCGCGACTGGTCCCTGCGTTATTAATCATTCACTCAAAGCCAGCGAAGCTTCCCGGGTGGCTGTGCTTAGTGCAATGGAGCCTCTTAGTTATCACCCGAACGCCAACGCCCGTGCGC‑GGGCGCGCCAGACCACTCAAAcggtagctac                                             <  2:607957/149‑7 (MQ=255)
                                                                                                          tCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGGGC‑TGGCGCCGCAGACCACTGACACGGTCGGCCTg                                            >  2:341525/1‑149 (MQ=255)
                                                                                                           cGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGC‑TGGCGCAGCAGACCACTGAAACggtcggtccgc                                           <  2:296277/149‑4 (MQ=255)
                                                                                                           acccACCGTTCCACGCGTGATTAATAATTCACCAAGAGCCAGCGAAGCTTCCCGGCGGGCTGTGCATAGTGCAATGGAGTCTATTAGCTATCACCCGAACGCCAACGCCCGTGCGC‑TGGCGCAGCAGACCACTGAAACGGTCGGTCTgg                                           <  2:245688/147‑1 (MQ=255)
                                                                                                             ccACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGC‑TGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTc                                         >  2:299546/1‑149 (MQ=255)
                                                                                                                       ccGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCAGGCTGGCTGTGCGTAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGC‑TGGCGGCGCAGACAACTGAAAAGGTCGGTCTGGTCGTTGGTGATg                               >  1:454459/1‑149 (MQ=255)
                                                                                                                               ttAATAATTCACCCAAAGCCATCGATGCTTCCCGGCTGGCTGTGCAAAGTGCAATGGAGTCTCTTAGATATCACCCGAACGCCAACGCCCGTGCGC‑TGGCGCAGCAGACCACTGAAACGGTCGATCTGGTCGTTGGTGATGTCTCCGAt                       >  1:629125/1‑149 (MQ=255)
                                                                                                                                 aataagTCACCCAAAGCCATCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGC‑TGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATcc                     <  1:284021/149‑1 (MQ=255)
                                                                                                                                         aCCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGC‑TGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCg             <  1:415896/149‑1 (MQ=255)
                                                                                                                                                 ccAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGC‑TGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATg     <  1:460085/149‑1 (MQ=255)
                                                                                                                                                   aGCGAACCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACACGAACGCCAACGCCCGTGCGC‑TGGCGCAGCGGACCACTGAAACGGTCGGTCTGGGCGTTGGTGGGGTTTCCGATCCGTTTTTCGGTGAAATGGt   >  2:294325/1‑149 (MQ=255)
                                                                                                                                                    gCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGC‑TGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGTg  >  1:192966/1‑149 (MQ=255)
                                                                                                                                                    gCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGACCGTGCGC‑TGGCGCAGCAGACCACTGAAACGGTCGGCGTGGTCGTTGGGGATGTTTCCGACGCGTTTTTCGCTGCAATgggg  >  2:227359/1‑147 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GAACCAACGGGCGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTTTCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGC‑TGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGTG  >  NC_000913_3_hsa_tpiA/2976536‑2976832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A