Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F29 I0 R1
|
32 |
37.4 |
1321382 |
89.2% |
1178672 |
148.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913_3_hsa_tpiA |
2,976,684 |
G→T |
19.0% |
S29I (AGC→ATC) |
galR → |
galactose‑inducible d‑galactose regulon transcriptional repressor; autorepressor |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913_3_hsa_tpiA | 2,976,684 | 0 | G | T | 19.0%
| 63.5
/ 7.7
| 36 | S29I (AGC→ATC) | galR | galactose‑inducible d‑galactose regulon transcriptional repressor; autorepressor |
| Reads supporting (aligned to +/- strand): ref base G (7/22); new base T (3/4); total (10/26) |
| Fisher's exact test for biased strand distribution p-value = 3.70e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01 |
AACCAACGGGCGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTTTCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGC > NC_000913_3_hsa_tpiA/2976537‑2976825
|
acccAACGGGCGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTTGCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGc < 2:158952/147‑1 (MQ=255)
cagcGGGCGTTTTCCGTAACACTGATAGCACGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTTTCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCATCGaa < 2:511528/146‑1 (MQ=255)
attCCGGAAACCTGAAAGAAGGTAAGCGTTTACCCACTAAGGTATTTGCATGGCGACCACAAAGGATGTAGCCCGACTGGCAGGCGTTGCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCggc < 2:279764/148‑1 (MQ=255)
cGTAACACGTAAAGAATGTAAGCGTTGACGCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTTTCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCATCGAAGCTTCCCggctggc < 1:508949/149‑1 (MQ=255)
tAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTTGCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGgctggctg > 1:192242/1‑149 (MQ=255)
aaCCCTGAAAGATTGTAAGCGTTTGCGCACTGTGGTATTTGCAGTGGGACCATAAAGGATGTAGCCCGACTGGCAGGCGTTTCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCtgt < 2:69883/149‑1 (MQ=255)
cTGAAAGAATGCAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTTGCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCAt < 1:42673/149‑1 (MQ=255)
tGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTTGCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAACCAGCGAAGCTTCCCGGCTGGCTGTGCATa < 2:98388/149‑1 (MQ=255)
tGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGTTGTAGCCCGACTGGCAGGCGTTGGAGGCACCGCTTTTTCCCGCGTCATTAATAAGTCACCCAGAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATg < 1:255295/149‑1 (MQ=255)
tatgCGTTTACCCACTAGGGTATTTTCAGGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGGTGCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGa < 2:66967/146‑1 (MQ=255)
aGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTTGCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGt < 1:602240/149‑1 (MQ=255)
aGCGTTTACCACCTAAGGTATTTGCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTTTCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTTCCGGCTGGCTGTGCATAGTGCAATGGAGt < 1:171373/149‑1 (MQ=255)
gCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTTGCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGtc < 1:462251/149‑1 (MQ=255)
ccACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTTGCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCt > 2:619103/1‑149 (MQ=255)
gcggctgtAAAGGATGTAGCCCGACTGACAGGCTTTGCAGGCTCCACCGTTTCCCGCGTCATTAATAATTCACCCAATGCCAGCGAATCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGaacgccaacgc < 2:190751/142‑1 (MQ=255)
gACCATAAAGGATGTAGCCCGACTGGCAGGCGTTTCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCATCGAAGCTTCCCGACTCGCTGTGCAGCGTGCAACGGGGTCCCTTAGCTATCACCCGcgcgccaacgcac > 1:313464/1‑147 (MQ=255)
aCCATAAAGGATGTAGCCCGACTGGCAGGCGTTTCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCATCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCCTACCTAGCACCCGAACGCCAACGCCCg > 1:51437/1‑149 (MQ=255)
aTAAAGGATGTAGCCCGACTGGCAGGCGTTGCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTgc < 1:605263/149‑1 (MQ=255)
tAGCGCGACTGGCAGGCGTTGCATTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCgcag < 1:268360/149‑1 (MQ=255)
ggccGGCTTGCAGGTGGTGCAGGCGCCGCCGTTACGCGCGGAATTAATAAGTCAGCAAGAGCCAGCGCGGCTGCGCGGCTGGCTGTGCAGAGGGCAATGGAGTGTGTTAGCTATCGCCCGAACGCCAACGCCCGTGCGCTGGCgcagca < 2:568075/147‑1 (MQ=255)
aGGCGTTGCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACACCTGaaa > 2:225526/1‑149 (MQ=255)
gtcggttCAGTCGCCACCGTTGCCCGCGTCATTAAGAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGTCTGTGCATAGGTCAATGGAGTCTCTTAGCTATCACCCGAACGCCCACGCCCGTGCGCTGGCGCAGCAGACCACTGAAAc < 2:359788/144‑1 (MQ=255)
ggCGTTGCTCTCTCCACCCTTTCCCGCGTCATTAATAATTCCCCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAAc < 2:265484/149‑1 (MQ=255)
gCGTTTCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCATCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAAcg > 2:267549/1‑149 (MQ=255)
aGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCATCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACggtcggtc < 1:358954/149‑1 (MQ=255)
tCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTg > 1:380235/1‑149 (MQ=255)
cGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTgg < 1:553724/149‑1 (MQ=255)
ccACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCGGCAGACCACTGAAACGGTCGGTCTGGTc > 2:405632/1‑149 (MQ=255)
cGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTg < 1:591320/149‑1 (MQ=255)
cccGAGTCATTAATAATTCACCCAAAGCCATCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGAt < 2:625069/149‑1 (MQ=255)
tCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATCGTGCAATGGAGACTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTcc < 1:353122/149‑1 (MQ=255)
aataatTCTCCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATcc < 1:412487/149‑1 (MQ=255)
taatTCACCCACAGCCAGCGAAGCTTCCCGGCCGGCTGTTCATAGTGCAATGGAGTCTCTAAGCTACCACCCGAACCCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGt < 1:143360/149‑1 (MQ=255)
ccAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGt > 2:185113/1‑149 (MQ=255)
ccAAAACCAGCGAAGCTCCCCCGCTGGCTGTGCATAGGGCAATGGAGTCTCGTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAATCGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGt < 2:219076/149‑1 (MQ=255)
aaaGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGGGTGTTAGCTATCCCCCGAACGCCAAGGCACGTGCGCTGGGGCAGCAGACCACTACAACGGTCGGTCTGGGCCGGGGTGAGGGTACCGATCCGCTGTTAGGtag > 2:238887/1‑147 (MQ=255)
|
AACCAACGGGCGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTTTCAGTCGCCACCGTTTCCCGCGTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGC > NC_000913_3_hsa_tpiA/2976537‑2976825
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
