Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F6 I0 R1
|
31 |
40.7 |
1457906 |
90.0% |
1312115 |
147.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913_3_hsa_tpiA |
2,795,396 |
C→A |
100% |
Q382K (CAG→AAG) |
gabP → |
gamma‑aminobutyrate transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913_3_hsa_tpiA | 2,795,396 | 0 | C | A | 100.0%
| 112.8
/ NA
| 37 | Q382K (CAG→AAG) | gabP | gamma‑aminobutyrate transporter |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (16/21); total (16/21) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
TTACTCTCCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGGCGCAACAGTTAG > NC_000913_3_hsa_tpiA/2795267‑2795540
|
ttACTCTCCACCGGCTCGGCATCCTTAACGGTGGTGGTGAACTATTCCGCTCTTGCGAAGGTATTTGAGTTTCTGATCGACAGCTTGTGCGCTATCGCGTTGATGGTTTTTGTCGTCGTCTCATTTTAAAAGTTGCGGATGCGCAAAAt > 2:141510/1‑149 (MQ=255)
gtgcgGCATTGTTAACGGTGGTGGTGAACTATTACGTGCCTGCGAAGGTATTTAACTTTCTGATCGACAGCGCCGGCGCCATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAgaa < 1:14354/147‑1 (MQ=255)
cggcgGCAGTCTTGACGGTTGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTGTGATCGACAGCTCCGGCGCTATGGCACTGCTGGTCTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAgaa < 2:726118/147‑1 (MQ=255)
gCATTCTTAACGGTGGTGGGGAACTATTACGCGCCTGCGAAGGTATTTAAATTTTTGATCGACAGCTCAGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAgacggaag < 2:448007/149‑1 (MQ=255)
cTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAACTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCTTCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCCGAAGGAGGCGcaa > 2:549607/1‑149 (MQ=255)
gtgAACTATTACGCGCCTGCGCAGGTAGTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGGAATTAGGTTgagctt > 1:70226/1‑144 (MQ=255)
aaCTATTACGCGCCTGCGAAGGTATTTAAATTTCGGATCGACAGCTCCGGCGCCATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTACGGATGGGCAAACTTCTGCGAGCCGAATGAAGCGCAATTCGCTTGCGGAtgtg > 2:46277/1‑149 (MQ=255)
tATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCt < 1:86572/149‑1 (MQ=255)
tACGCGCCTGCGAATGTATTTAAATTTCTGATCGACAGTTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTa < 2:713182/149‑1 (MQ=255)
ccTGCGAAGGTATTTAAATTTCTGATCGACAGATCCGGCGCAATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCTGATGCGCAAAACTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTg < 1:63119/149‑1 (MQ=255)
cTGCGAAGGCATTTAAATTTCTCATCGACAGCTCCGGCTCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTGTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTgg < 2:70226/149‑1 (MQ=255)
tGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCCCCTGTGCTCAGTCCTAGGAGCAGAAGGAAGCGCAATTCGCTTGCGAATGGGGATCTATCCGTAGc > 1:167184/1‑149 (MQ=255)
aaGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTccc > 1:320740/1‑147 (MQ=255)
ggTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCt < 2:14595/149‑1 (MQ=255)
aTTTAAATTTCTGATCGACAGCTCCGGCGCTAAGGGCCTGCTGGTTTATTTAGTCCTCGCCGTTTCAAAATTGCGAATGCGCAAAATTCTGCGAGCAGAAGGAAGCAAAATTCGCTTGCGAATATGGCTTTCGCGTTGGCTCCCCTGGc > 2:239438/1‑149 (MQ=255)
ttAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTa < 1:186504/149‑1 (MQ=255)
cTGATCGACAGCTCCGGCGCTATCGGCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGACGCTAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCgg < 2:83408/149‑1 (MQ=255)
gaccggcAGCTCCGGCGCTATTGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCATAAGGACGCGAAATTCGCTTGCGAATGTGGCTTTATCCGGGGCTCACCTGGCTAGTCATCGGCt < 1:5643/143‑1 (MQ=255)
gATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTAACCTAGCTAGTCATCGGCt > 1:275969/1‑149 (MQ=255)
aTCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCtt < 1:269397/149‑1 (MQ=255)
cGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTtta < 2:573556/149‑1 (MQ=255)
cTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTAcat > 1:637753/1‑147 (MQ=255)
cTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCt > 1:702927/1‑149 (MQ=255)
ccGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCttt < 1:558324/149‑1 (MQ=255)
ggCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTtgt > 2:310044/1‑149 (MQ=255)
cgcTATCGCGCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTgtgt < 2:424572/149‑1 (MQ=255)
cgcTATCGCCCGTGTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTgtgt < 2:65535/149‑1 (MQ=255)
tATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTgtgt > 2:105557/1‑146 (MQ=255)
tATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTgtgt < 1:105557/146‑1 (MQ=255)
ccctctgGTTTATTTAGTCATCGCCTTTTAAAAGTTGCGGATGATCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCTCTTGCCAATGTGGCTTTATCCGTGGCTCGCGTGGCTATTCATAGGCCTTATTACCTTTGTGTTGGTAGTGa < 2:123370/145‑1 (MQ=255)
ccTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGa < 1:243107/149‑1 (MQ=255)
gctgGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGc < 1:669576/149‑1 (MQ=255)
gcgggTTTATTTAGTCATCGCCGTTTCAAAGGTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGc < 2:466869/146‑1 (MQ=255)
gTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGgc > 1:31168/1‑149 (MQ=255)
tCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTCTGTGTTGGTATTGATGCTATTCCGTCCGgcg > 2:653973/1‑149 (MQ=255)
tCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCCTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGGCGCaa > 2:418852/1‑149 (MQ=255)
ttCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGGCGCAACAGTTAg > 1:188950/1‑149 (MQ=255)
|
TTACTCTCCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGGCGCAACAGTTAG > NC_000913_3_hsa_tpiA/2795267‑2795540
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
