Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F4 I1 R1
|
17 |
32.4 |
1141670 |
89.0% |
1016086 |
148.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913_3_hsa_tpiA |
2,795,396 |
C→A |
Q382K (CAG→AAG) |
gabP → |
gamma‑aminobutyrate transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913_3_hsa_tpiA | 2,795,396 | 0 | C | A | 94.1%
| 84.5
/ ‑6.1
| 34 | Q382K (CAG→AAG) | gabP | gamma‑aminobutyrate transporter |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base A (13/19); total (14/20) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CGTGGCGGTGTTACTCTCCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGGCGCAACAGTTA > NC_000913_3_hsa_tpiA/2795257‑2795539
|
cGTGGCGGTGTTACTCTCCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGa < 1:458305/149‑1 (MQ=255)
aCTCTCCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGGTTCAACGTTGCGGATGCGCAAAATTc > 1:47939/1‑149 (MQ=255)
tctcCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTTATCGACAGCTCCGGCGTTATCGCCCTGCTGGTTTCTTTAGTCATCTCCGCTTCAAACTTGCGGATGGTCAAAATTccg > 2:40507/1‑147 (MQ=255)
tcCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGATTCAAAGTTGCGAGTGCGCAAAATTCTGCg > 1:267600/1‑149 (MQ=255)
gcgcGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAgaa < 1:462673/149‑1 (MQ=255)
gCATTCTTAACGGTGGTGGTGAATGATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAgaaggaag < 2:352300/149‑1 (MQ=255)
aTTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCAGCAAGCGTAAGGGAGCg > 2:371274/1‑149 (MQ=255)
ttCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTATGCGAGCAAAAGGAAGCGa > 2:494590/1‑149 (MQ=255)
cTTAACGGTGGTGGTGAACTATTACGCTCCTGTGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGaaa < 1:149698/149‑1 (MQ=255)
ttAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAAt > 1:92009/1‑149 (MQ=255)
tggtggtgAAGTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATTGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTg < 2:307290/149‑1 (MQ=255)
cTAGTACGCGCCTGCGAAGGTATTTAATTTTCTGATCGTCAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGc < 2:215772/149‑1 (MQ=255)
gcgcCTGCGAAGGTATTTAAATTTCTGCTCGACAGCTCCGGCGCTATCGCCCTACTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAATTTGGCTTGCGGATGTGGCTTTATcc > 1:369820/1‑149 (MQ=255)
aaGGTATTTAACTTTCTGATCTACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGCAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCAc < 2:415011/149‑1 (MQ=255)
aaGGTATGTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTAATTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCAc < 2:415013/149‑1 (MQ=255)
tttttAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTgg < 2:294125/147‑1 (MQ=255)
gATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCt < 1:12807/149‑1 (MQ=255)
cGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTtta < 1:323006/149‑1 (MQ=255)
gcgccGGCGCTAACGCCCTGTTGGTTTATTTAGTCATCGGCGTTTCACAGTTGCGGATGCGCAAAATTCTGCAAGCCGGAGGGCGTGAAATTCGCTTGTGAGATTGGTTTTATCCGTGGCTTACTTGGCGAGTCAGCGGCTTTATTAcc < 2:396878/146‑1 (MQ=255)
tCCGGTGTTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAATTTGCGTATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTttattcaccc < 2:149967/149‑6 (MQ=255)
tCCGGCGCTATCGCCCTGCTGGTTTATGTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCtt < 2:220410/149‑1 (MQ=255)
cTATCGCCCTGCTGGGTTATTTAGTCATCGCAGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTCTTACCTTTGTGTTg > 2:497773/1‑149 (MQ=255)
tcttcatgccgGTTTATTTATGCATCGCGGTTGCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGGCGGAAGCGAAATTGGCTTGCGAATGTGGCTGTATCCGTGGCTCACCTGGCTAGTTATCGGTTTTATTACCTTTGTGTTGGTa < 2:163158/143‑1 (MQ=255)
tCGCCCTGTTTGTGTATGTAGTCATAGGGTTTTTAAAGGTGCGGATGCGCAAAATTTTTCGGGCAGAAGGAAGCGAAATTCGCTTGCGATTGTTGCTTTATCCGTGGCTTACCTGGCTAGTCATCGGCTTTATTACTTTTGTGTTGGTa < 2:116660/149‑1 (MQ=255)
gCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGACGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTGCCTTTGTGTTGGTAGt > 1:362301/1‑149 (MQ=255)
ccTGCGTGTTTATTTAGTGATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGCAATTCGCTCGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTGATTACCTTTGTGTTGGTAGTGa < 2:333619/149‑1 (MQ=255)
gTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCATTGTGTTGGTAGAGAAGCTAtc > 2:486638/1‑148 (MQ=255)
cttatttaGACAACACCGTTTCCCAGTTGCGGATGCGCAAAAGTCTGCGAGGTAAAGTAAGCGAAATGCGTTGGCTAATGTGGCTTTATCCGTGGCTCAGCTGGCTAGTCATCGGCTTTATTACCTAGGTCGTGGTAGTGATGCTAtcc > 2:333613/2‑147 (MQ=255)
ttatttaGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGGTGCTATtcc > 1:171805/1‑149 (MQ=255)
gTCATCGACGTTTCAAAGTTGCGCATGCGCAAAATTCTGCCGTCACAAGGAAACGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGgc < 2:100108/149‑1 (MQ=255)
aTCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTCCGAATGTGGCTTTATCCGTAGCTCACCTGGCTAGTCATCGGCGTTATTGCCTTTGTGTTGGTCATGTTGCTATTGCGTCGCGCGCa > 2:336179/1‑149 (MQ=255)
ccGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGGCGCAACAg > 2:10506/1‑149 (MQ=255)
tttCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGGAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGGCGCAACAGTTa < 1:378822/149‑1 (MQ=255)
tttCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGGCGCAACAGTTa < 1:461308/149‑1 (MQ=255)
|
CGTGGCGGTGTTACTCTCCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGGCGCAACAGTTA > NC_000913_3_hsa_tpiA/2795257‑2795539
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
