Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F66 I1 R1	15	85.2	3457112	93.8%	3242771	129.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_bme_pgi	4,158,779	T→G	L97W (TTG→TGG)	oxyR →	oxidative and nitrosative stress transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_bme_pgi	4,158,779	0	T	G	96.0%	258.3 / 0.3	76	L97W (TTG→TGG)	oxyR	oxidative and nitrosative stress transcriptional regulator
Reads supporting (aligned to +/- strand): ref base T (1/2); new base G (38/35); total (39/37)
Fisher's exact test for biased strand distribution p-value = 6.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

GAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCC  >  NC_000913_3_bme_pgi/4158634‑4158925

gAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTGGAtt                                                                                                                                                >  2:663668/1‑150 (MQ=255)

  gCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTGGATTcc                                                                                                                                              <  3:302728/150‑1 (MQ=255)

   cGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTGGATTccc                                                                                                                                             >  1:219708/1‑150 (MQ=255)

   cGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTGGATTccc                                                                                                                                             <  2:855558/150‑1 (MQ=255)

    ggACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTGGATTCCca                                                                                                                                            <  2:306147/150‑1 (MQ=255)

    ggACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTGGATTCCca                                                                                                                                            >  3:561355/1‑150 (MQ=255)

        cAGCCGTAAAGTGTTGTTCACCCAAGCGGGAATGATGCTGGTGGATCAGGCGCGTAACGTACGGCGTGAGGGGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTGGATTCCCACAGt                                                                                                                                        >  2:268547/1‑150 (MQ=255)

             gTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTGGATTCCCACAGTTGGaa                                                                                                                                   <  3:310743/150‑2 (MQ=255)

                  gtgtTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGAGGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTGGATTCCCACAGTTGGACCGTAc                                                                                                                              <  4:624429/150‑1 (MQ=255)

                        ttCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTGGATTCCCACAGTTGGACCGTACCTGCTa                                                                                                                        >  1:272761/1‑150 (MQ=255)

                        ttCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTGGATTCCCACAGTTGGACCGTACCTGCTa                                                                                                                        <  4:159338/150‑1 (MQ=255)

                               aGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTGGATTCCCACAGTTGGACCGTACCTGCTACCGCata                                                                                                                 <  2:941133/150‑1 (MQ=255)

                                     gAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTGGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATcc                                                                                                           <  1:433524/150‑1 (MQ=255)

                                           tgctgGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTGGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTAtgc                                                                                                     >  3:608739/1‑150 (MQ=255)

                                           tgctgGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTGGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTAtgc                                                                                                     >  4:227617/1‑150 (MQ=255)

                                            gctgGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTGGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTAtgct                                                                                                    >  1:427032/1‑150 (MQ=255)

                                             ctgGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTGGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTAtgctg                                                                                                   >  4:191280/1‑150 (MQ=255)

                                              tggtggATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTGGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTAtgctgc                                                                                                  <  1:232135/150‑1 (MQ=255)

                                              tggtggATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTGGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTAtgctgc                                                                                                  >  1:299526/1‑150 (MQ=255)

                                                   gATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTGGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAg                                                                                             >  4:410839/1‑150 (MQ=255)

                                                       aGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTGGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCt                                                                                         >  1:877453/1‑150 (MQ=255)

                                                         gcgcGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTGGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCttt                                                                                       >  1:886835/1‑150 (MQ=255)

                                                         gcgcGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGCCCGGACCGCTGCACATTGGTTGGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCttt                                                                                       >  4:556065/1‑150 (MQ=255)

                                                          cgcgTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTGGATTCCCACAGTTGGACCGTACCTGCTACCGCATAGTATCCCTATGCTGCACCAGACCttcc                                                                                      <  2:180337/150‑3 (MQ=255)

                                                             gTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTGGAt                                                                                                                                                 <  4:343509/88‑1 (MQ=255)

                                                             gTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTGGAt                                                                                                                                                 >  3:343509/1‑88 (MQ=255)

                                                                     ctgcGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTGGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGATCTTTCCAAAGCTGGaa                                                                           <  3:521857/150‑1 (MQ=255)

                                                                     ctgcGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTGGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGaa                                                                           <  1:663668/150‑1 (MQ=255)

                                                                     ctgcGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTGGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGaa                                                                           >  1:688820/1‑150 (MQ=255)

                                                                      tgcGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTGGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGaaa                                                                          <  1:719121/150‑1 (MQ=255)

                                                                       gcGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAAt                                                                         <  4:61211/150‑1 (MQ=255)

                                                                       gcGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTGGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCAc                                                                                                >  4:333283/1‑127 (MQ=255)

                                                                       gcGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTGGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCAc                                                                                                <  3:333283/127‑1 (MQ=255)

                                                                            aGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTGGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATc                                                                    >  2:111213/1‑150 (MQ=255)

                                                                                 aaaGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTGGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCAt                                                               <  3:410839/150‑1 (MQ=255)

                                                                                    gTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTGGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGaa                                                            <  3:634976/150‑1 (MQ=255)

                                                                                      ccTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTGGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGc                                                          <  4:608739/150‑1 (MQ=255)

                                                                                      ccTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACAATGGTTGTATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGc                                                          <  1:268547/150‑1 (MQ=255)

                                                                                       cTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTGGATTccc                                                                                                                                             >  1:639232/1‑66 (MQ=255)

                                                                                       cTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTGGATTccc                                                                                                                                             <  2:639232/66‑1 (MQ=255)

                                                                                        ttAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTGGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCt                                                                   <  4:21020/139‑1 (MQ=255)

                                                                                        ttAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTGGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCt                                                                   >  3:21020/1‑139 (MQ=255)

                                                                                         tAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTGGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGaa                                                            <  1:236486/145‑1 (MQ=255)

                                                                                         tAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTGGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGaa                                                            >  2:236486/1‑145 (MQ=255)

                                                                                            agagATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTGGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGAc                                                    <  3:191280/150‑1 (MQ=255)

                                                                                              agaTGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTGGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGAccc                                                  >  4:66732/1‑150 (MQ=255)

                                                                                                  ggCAAGCCAGCAGGGCGAGACGATGTCCGGACCTCTGCACATTGGTTGGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGa                                                                                            <  4:608413/104‑1 (MQ=255)

                                                                                                  ggCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTGGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGa                                                                                            >  3:608413/1‑104 (MQ=255)

                                                                                                    cAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTGGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGt                                            >  1:717466/1‑150 (MQ=255)

                                                                                                     aaGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTGGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGtt                                           >  3:322203/1‑150 (MQ=255)

                                                                                                       gCCAGCAGGGCGAGACGATGCCCGGACCGCTGCACATTGGTTGGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTAc                                         <  3:556065/150‑1 (MQ=255)

                                                                                                             agGGCGAGACGATGTCCGGACCGCTGCACATTGGTTGGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGgcgc                                   >  1:340535/1‑150 (MQ=255)

                                                                                                              gggCGAGACGATGTCCGGACCGCTGCACATTGGTTGGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCa                                  <  2:219708/150‑1 (MQ=255)

                                                                                                                gCGAGACGATGTCCGGACCGCTGCACATTGGTTGGATTCCCACAGTTGGACCGTACCTGCTACCGCAtattat                                                                                                             <  4:522555/73‑1 (MQ=255)

                                                                                                                gCGAGACGATGTCCGGACCGCTGCACATTGGTTGGATTCCCACAGTTGGACCGTACCTGCTACCGCAtattat                                                                                                             >  3:522555/1‑73 (MQ=255)

                                                                                                                    gaCGATGTCCGGACCGCTGCACATTGGTTGGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGa                            <  2:717466/150‑1 (MQ=255)

                                                                                                                    gaCGATGTCCGGACCGCTGCACATTGGTTGGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGa                            <  2:299526/150‑1 (MQ=255)

                                                                                                                       gATGTCCGGACCGCTGCACATTGGTTGGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTc                                                                                      >  1:87516/1‑89 (MQ=255)

                                                                                                                       gATGTCCGGACCGCTGCACATTGGTTGGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTc                                                                                      <  2:87516/89‑1 (MQ=255)

                                                                                                                        aTGTCCGGACCGCTGCACATTGGTTGGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGc                        >  2:44290/1‑150 (MQ=255)

                                                                                                                         tGTCCGGACCGCTGCACATTGGTTGGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCg                       <  2:688820/150‑1 (MQ=255)

                                                                                                                           tCCGGACCGCTGCACATTGGTTGGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGtt                                           >  2:969148/1‑128 (MQ=255)

                                                                                                                           tCCGGACCGCTGCACATTGGTTGGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGtt                                           <  1:969148/128‑1 (MQ=255)

                                                                                                                               gACCGCTGCACATTGGTTGGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAAc                 >  1:698589/1‑150 (MQ=255)

                                                                                                                                  cGCTGCACATTGGTTGGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCg              >  1:783753/1‑150 (MQ=255)

                                                                                                                                  cGCTGCACAGTGGTTGGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCg              >  4:458428/1‑150 (MQ=255)

                                                                                                                                    cTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTgg                             >  4:94497/1‑133 (MQ=255)

                                                                                                                                    cTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTgg                             <  3:94497/133‑1 (MQ=255)

                                                                                                                                        acaTTGGTTGGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCg        <  1:44290/150‑1 (MQ=255)

                                                                                                                                        acaTTGGTTGGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGAAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCg        <  2:698589/150‑1 (MQ=255)

                                                                                                                                         caTTGGTTGGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGt       >  2:252134/1‑150 (MQ=255)

                                                                                                                                             ggTTGGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTgg                                                                             >  1:271702/1‑76 (MQ=255)

                                                                                                                                             ggTTGGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTgg                                                                             <  2:271702/76‑1 (MQ=255)

                                                                                                                                             ggTTGGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATc   >  3:245167/1‑150 (MQ=255)

                                                                                                                                              gTTGGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAAc                                >  1:252746/1‑120 (MQ=255)

                                                                                                                                              gTTGGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCGGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATcc  >  2:176752/1‑150 (MQ=255)

                                                                                                                                               tttGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTCTGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGCTACTGGCGCAACTGGACAGCGGCAAACTCGATTGc         >  3:295563/1‑142 (MQ=255)

                                                                                                                                               tttGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGc         <  4:295563/142‑1 (MQ=255)

GAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCC  >  NC_000913_3_bme_pgi/4158634‑4158925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A