Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A17 F133 I1 R1	18	54.6	2042212	95.3%	1946228	140.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_tpiA	1,726,004	G→A	intergenic (‑84/‑19)	ydhL ← / → nemR	DUF1289 family protein/transcriptional repressor for the nemRA‑gloA operon, quinone‑, glyoxal‑, and HOCl‑activated

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	1,726,004	0	G	A	98.8%	292.6 / ‑4.5	83	intergenic (‑84/‑19)	ydhL/nemR	DUF1289 family protein/transcriptional repressor for the nemRA‑gloA operon, quinone‑, glyoxal‑, and HOCl‑activated
Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (47/35); total (48/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

TCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGGTCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAG  >  NC_000913_3_pae_tpiA/1725859‑1726149

tCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATcta                                                                                                                                               >  1:416707/1‑150 (MQ=255)

 cAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCGACTTTGCTTGCACTAGACCGACTGATctac                                                                                                                                              <  3:280661/150‑1 (MQ=255)

      tGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTacac                                                                                                                                         >  4:239662/1‑150 (MQ=255)

      tGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTacac                                                                                                                                         >  2:136524/1‑150 (MQ=255)

      tGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTacac                                                                                                                                         <  1:465702/150‑1 (MQ=255)

        gCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTc                                                                                                                                       >  1:80693/1‑150 (MQ=255)

           aaTTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCaa                                                                                                                                    <  1:518267/150‑1 (MQ=255)

                cccGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCAt                                                                                                                               <  3:339032/150‑1 (MQ=255)

                cccGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCAt                                                                                                                               <  1:460183/150‑1 (MQ=255)

                      aCGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGaacaa                                                                                                                         <  1:589695/150‑1 (MQ=255)

                        gggCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGaacaaac                                                                                                                       >  4:92258/1‑150 (MQ=255)

                              tGGACGGGAAAGAACTCTAATTGCTCCCCCACTCAGTCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGGTCTACTACACTCCAACGCATGAACAAACACACCg                                                                                                                 >  4:195912/1‑150 (MQ=255)

                                      aaaGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGAt                                                                                                         >  1:457345/1‑150 (MQ=255)

                                       aaGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATa                                                                                                        <  3:239662/150‑1 (MQ=255)

                                          aaCTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTc                                                                                                     >  2:554072/1‑150 (MQ=255)

                                             tctAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTcgcg                                                                                                  >  2:375341/1‑150 (MQ=255)

                                               tAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGaa                                                                                                <  2:463456/150‑1 (MQ=255)

                                               tAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGaa                                                                                                <  1:554072/150‑1 (MQ=255)

                                                     cTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACAtctc                                                                                          >  2:339523/1‑150 (MQ=255)

                                                          ccACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGGTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGc                                                                                     >  2:60991/1‑150 (MQ=255)

                                                              ttCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCTAcc                                                                                 >  3:278423/1‑149 (MQ=255)

                                                               tCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACgg                                                                                <  1:419561/150‑1 (MQ=255)

                                                                      cctcAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCa                                                                         >  1:20027/1‑150 (MQ=255)

                                                                      catcAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCa                                                                         >  1:9177/3‑150 (MQ=255)

                                                                       ctcAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCaa                                                                        >  2:381573/1‑150 (MQ=255)

                                                                         cAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGaa                                                                                                >  3:348303/1‑124 (MQ=255)

                                                                         cAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGaa                                                                                                <  4:348303/124‑1 (MQ=255)

                                                                          aGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAacacac                                                                                                                   <  1:235202/104‑1 (MQ=255)

                                                                          aGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAacacac                                                                                                                   >  2:235166/1‑104 (MQ=255)

                                                                          aGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAacacac                                                                                                                   >  2:235202/1‑104 (MQ=255)

                                                                          aGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAacacac                                                                                                                   <  1:235166/104‑1 (MQ=255)

                                                                             tAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGc                                                                  <  2:416707/150‑1 (MQ=255)

                                                                              aaGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGcc                                                                 >  2:141901/1‑150 (MQ=255)

                                                                                gATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTg                                                               >  3:252411/1‑150 (MQ=255)

                                                                                   tattaCCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCaa                                                            >  4:54595/1‑150 (MQ=255)

                                                                                      taCCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGt                                                         <  2:9177/150‑1 (MQ=255)

                                                                                         cATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGa                                                      >  2:228165/1‑150 (MQ=255)

                                                                                         cATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGa                                                      >  2:228272/1‑150 (MQ=255)

                                                                                             attGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCa                                                  >  2:367536/1‑150 (MQ=255)

                                                                                             attGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCa                                                  >  2:192908/1‑150 (MQ=255)

                                                                                                gAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCg                                               <  4:252411/150‑1 (MQ=255)

                                                                                                 aaGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCgg                                              >  1:102210/1‑150 (MQ=255)

                                                                                                 aaGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCgg                                              <  3:92258/150‑1 (MQ=255)

                                                                                                   gCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGaa                                                                                                               <  1:489878/83‑1 (MQ=255)

                                                                                                   gCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGaa                                                                                                               >  2:489878/1‑83 (MQ=255)

                                                                                                     tGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATg                                          >  2:540967/1‑150 (MQ=255)

                                                                                                      gTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATgg                                         <  3:54595/150‑1 (MQ=255)

                                                                                                       ttAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATggg                                        >  4:158394/1‑150 (MQ=255)

                                                                                                       ttAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATggg                                        >  1:13959/1‑150 (MQ=255)

                                                                                                        tAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATgggg                                       <  3:158394/150‑1 (MQ=255)

                                                                                                         aaTGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGc                                      >  3:95480/1‑150 (MQ=255)

                                                                                                             tCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAg                                  <  1:226612/150‑1 (MQ=255)

                                                                                                              ccAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGc                                 <  1:141901/150‑1 (MQ=255)

                                                                                                                 aaGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCt                                                                <  3:47738/116‑1 (MQ=255)

                                                                                                                 aaGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCt                                                                >  4:47738/1‑116 (MQ=255)

                                                                                                                 aaGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGaa                              <  3:256567/150‑1 (MQ=255)

                                                                                                                 aaGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGaa                              <  4:306442/150‑1 (MQ=255)

                                                                                                                  aGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCCCCGGGATGGGGCTAAGCGAAt                             >  2:171076/1‑150 (MQ=255)

                                                                                                                      gCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGa                                                                                                          <  1:274797/69‑1 (MQ=255)

                                                                                                                      gCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGa                                                                                                          >  2:274797/1‑69 (MQ=255)

                                                                                                                       cAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTa                        >  3:153713/1‑150 (MQ=255)

                                                                                                                       cAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTa                        <  1:60991/150‑1 (MQ=255)

                                                                                                                        aaCTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGt                                                         <  1:270766/116‑1 (MQ=255)

                                                                                                                        aaCTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGt                                                         >  2:270766/1‑116 (MQ=255)

                                                                                                                        aaCTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTaa                       >  1:299314/1‑150 (MQ=255)

                                                                                                                            ttgcttgcACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAAc                   <  1:136524/150‑1 (MQ=255)

                                                                                                                            ttgcttgcACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAAc                   <  1:367536/150‑1 (MQ=255)

                                                                                                                                 tgcACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTg              <  4:272794/150‑1 (MQ=255)

                                                                                                                                  gcACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGa             >  1:167878/1‑150 (MQ=255)

                                                                                                                                  gcACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGa             >  3:355671/1‑150 (MQ=255)

                                                                                                                                    aCTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATAATCGCGAACATCTCCTGGCGACGAGGGCTGAACGCGGGCGGCAACGCGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAg           >  3:273326/1‑150 (MQ=255)

                                                                                                                                     cTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGt          >  4:233357/1‑150 (MQ=255)

                                                                                                                                      tAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCAcc                                                <  3:273041/111‑1 (MQ=255)

                                                                                                                                      tAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCAcc                                                >  4:273041/1‑111 (MQ=255)

                                                                                                                                      tAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTg         >  2:574210/1‑150 (MQ=255)

                                                                                                                                        gACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAAt                             >  1:556112/1‑128 (MQ=255)

                                                                                                                                        gACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAAt                             <  2:556112/128‑1 (MQ=255)

                                                                                                                                        gACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGcc       >  3:240648/1‑150 (MQ=255)

                                                                                                                                         aCCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCg      >  2:29593/1‑150 (MQ=255)

                                                                                                                                            gACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGaaa   <  2:457345/150‑1 (MQ=255)

                                                                                                                                             aCTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGc                                                                  >  4:278420/1‑86 (MQ=255)

                                                                                                                                             aCTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGc                                                                  <  3:278420/86‑1 (MQ=255)

                                                                                                                                             aCTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAg  >  2:310041/1‑150 (MQ=255)

TCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGGTCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAG  >  NC_000913_3_pae_tpiA/1725859‑1726149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A