Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F80 I1 R1	130	60.2	1467357	98.1%	1439477	216.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,570,488	A→G	C422R (TGC→CGC)	eutE ←	putative aldehyde dehydrogenase, ethanolamine utilization protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,570,488	0	A	G	100.0%	12.7 / NA	5	C422R (TGC→CGC)	eutE	putative aldehyde dehydrogenase, ethanolamine utilization protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (4/1); total (4/1)

CGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTCCCCGCCCAGCCCCAGCCCGGCAATGCACGGTCCGTTCTTAACGAAAATGCTGGTATCAATAGCATTCGCCATCTGGTTCATGTTTTCGATGTTGCGCGAGTGCATTGCCGCCGTGTGGTGGCAACCGCCTTCCAGTTTCACCGCTAGCGCAATGGCATCCGCCACGTTGGCGACGCGCACGACGGGCAACACCGGCATCATCAGTTCAGTCACGGCAAACGGATGTTCTGCGGTGGTTTCCACAAACAGCAGGCGCGTT  >  NC_000913/2570385‑2570720

cGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTCCCCGCCCAGCCCCAGCCCGGCAATGCGCGGTCCGTTCTTAACGAAAATGCTGGTATCAATAGCATTCGCCATCTGGTTCATGTTTTCGATGTTGCGCGAGTGCATTGCCGCCGTGTGGTGGCAACCGCCTTCCAGTTTCACCGCTAGCGCAATGGCATCCGcc                                                                                                  >  2:473353/1‑240 (MQ=255)

                                                                                                gCAATGCGCGGTCCGTTCTTAACGAAAATGCTGGTATCAATAGCATTCGCCATCTGGTTCATGTTTTCGATGTTGCGCGAGTGCATTGCCGCCGTGTGGTGGCAACCGCCTTCCAGTTTCACCGCTAGCGCAATGGCATCCGCCACGTTGGCGACGCGCACGACGGGCAACACCGGcatcat                                                            <  2:582683/182‑1 (MQ=255)

                                                                                                gCAATGCGCGGTCCGTTCTTAACGAAAATGCTGGTATCAATAGCATTCGCCATCTGGTTCATGTTTTCGATGTTGCGCGAGTGCATTGCCGCCGTGTGGTGGCAACCGCCTTCCAGTTTCACCGCTAGCGCAATGGCATCCGCCACGTTGGCGACGCGCACGACGGGCAACACCGGCATCATCAGTTCAGTCACGGCAAACGGATGTTCTGCGGTGGTTTCCACAAACAGCAGGCGCGtt  >  1:253571/1‑240 (MQ=255)

                                                                                                gCAATGCGCGGTCCGTTCTTAACGAAAATGCTGGTATCAATAGCATTCGCCATCTGGTTCATGTTTTCGATGTTGCGCGAGTGCATTGCCGCCGTGTGGTGGCAACCGCCTTCCAGTTTCACCGCTAGCGCAATGGCATCCGCCACGTTGGCGACGCGCACGACGGGCAACACCGGCATCATCAGTTCAGTCACGGCAAACGGATGTTCTGCGGTGGTTTCCACAAACAGCAGGCGCGtt  >  1:373163/1‑240 (MQ=255)

                                                                                                gCAATGCGCGGTCCGTTCTTAACGAAAATGCTGGTATCAATAGCATTCGCCATCTGGTTCATGTTTTCGATGTTGCGCGAGTGCATTGCCGCCGTGTGGTGGCAACCGCCTTCCAGTTTCACCGCTAGCGCAATGGCATCCGCCACGTTGGCGACGCGCACGACGGGCAACACCGGCATCATCAGTTCAGTCACGGCAAACGGATGTTCTGCGGTGGTTTCCACAAACAGCAGGCGCGtt  >  2:162434/1‑240 (MQ=255)

CGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTCCCCGCCCAGCCCCAGCCCGGCAATGCACGGTCCGTTCTTAACGAAAATGCTGGTATCAATAGCATTCGCCATCTGGTTCATGTTTTCGATGTTGCGCGAGTGCATTGCCGCCGTGTGGTGGCAACCGCCTTCCAGTTTCACCGCTAGCGCAATGGCATCCGCCACGTTGGCGACGCGCACGACGGGCAACACCGGCATCATCAGTTCAGTCACGGCAAACGGATGTTCTGCGGTGGTTTCCACAAACAGCAGGCGCGTT  >  NC_000913/2570385‑2570720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A