Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F60 I1 R1	14	101.6	3468360	98.9%	3430208	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,443,976	A→T	I41N (ATC→AAC)	secY ←	Sec translocon subunit SecY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,443,976	0	A	T	98.2%	362.1 / NA	109	I41N (ATC→AAC)	secY	Sec translocon subunit SecY
Reads supporting (aligned to +/- strand): ref base A (0/0); major base T (55/52); minor base C (2/0); total (57/52)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 110 total aligned reads displayed.

ATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGATCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGTCTGCGTTTCAGCTCGCCTAAGCCACCTTTGGCACTTTGAAAATCTAATCCCGGTTGTTTAGCCATCTGCTACTTATTCCT  >  NC_000913/3443843‑3444112

ataCGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGaa                                                                                                                                    <  2:661322/140‑1 (MQ=255)

    ggCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATaaa                                                                                                                                <  1:1561235/140‑1 (MQ=255)

    ggCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATaaa                                                                                                                                >  1:439686/1‑140 (MQ=255)

    ggCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATaaa                                                                                                                                <  1:140675/140‑1 (MQ=255)

      cATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAag                                                                                                                              >  1:641226/1‑140 (MQ=255)

       aTGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAaga                                                                                                                             >  1:1573432/1‑140 (MQ=255)

         gATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATACAAGAGc                                                                                                                           >  2:1545778/1‑140 (MQ=255)

         gATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGc                                                                                                                           <  2:1028575/140‑1 (MQ=255)

         gATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGc                                                                                                                           >  2:1026384/1‑140 (MQ=255)

          aTCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGcc                                                                                                                          >  2:539592/1‑140 (MQ=255)

           tCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCa                                                                                                                         >  1:908189/1‑140 (MQ=255)

           tCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCa                                                                                                                         <  2:55188/140‑1 (MQ=255)

              ccAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATa                                                                                                                      <  1:1430344/140‑1 (MQ=255)

               cAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATAc                                                                                                                     <  1:648425/140‑1 (MQ=255)

               cAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATAc                                                                                                                     <  2:809603/140‑1 (MQ=255)

               cAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATAc                                                                                                                     <  1:220818/140‑1 (MQ=255)

                agagCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACg                                                                                                                    >  2:19237/1‑140 (MQ=255)

                agagCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACg                                                                                                                    >  1:659386/1‑140 (MQ=255)

                agagCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACg                                                                                                                    >  2:1451668/1‑140 (MQ=255)

                 gagCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACgg                                                                                                                   <  1:1102617/140‑1 (MQ=255)

                   gCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTGGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGaa                                                                                                                 <  2:345155/140‑1 (MQ=255)

                    cAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAac                                                                                                                <  1:1254897/140‑1 (MQ=255)

                     aaaGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAaca                                                                                                               >  1:426495/1‑140 (MQ=255)

                      aaGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAacac                                                                                                              <  2:1084912/140‑1 (MQ=255)

                      aaGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAacac                                                                                                              >  1:337901/1‑140 (MQ=255)

                            gAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGTATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAg                                                                                                        >  1:1371752/1‑140 (MQ=255)

                              aGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAgcg                                                                                                      <  2:1650443/140‑1 (MQ=255)

                              aGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAgc                                                                                                       >  1:671229/1‑139 (MQ=255)

                              aGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAgc                                                                                                       <  2:671229/139‑1 (MQ=255)

                               gCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAgcgc                                                                                                     <  1:1451668/140‑1 (MQ=255)

                                 aCGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCAc                                                                                                   <  2:439686/140‑1 (MQ=255)

                                   ggCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCg                                                                                                 >  1:1434700/1‑140 (MQ=255)

                                    gCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGa                                                                                                <  1:940659/140‑1 (MQ=255)

                                      tGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATa                                                                                              <  1:1635423/140‑1 (MQ=255)

                                          agCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATaacaa                                                                                          >  2:234137/1‑140 (MQ=255)

                                          agCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATaacaa                                                                                          >  2:1131636/1‑140 (MQ=255)

                                            caccacCAGAGAACATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATaacaaac                                                                                        >  2:131670/1‑140 (MQ=255)

                                             accaccAGAGAACATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATaacaaaca                                                                                       >  2:1292840/1‑140 (MQ=255)

                                             accaccAGAGAACATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATaacaaaca                                                                                       >  2:152441/1‑140 (MQ=255)

                                               caccaGAGAACATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAAcagc                                                                                     >  2:158585/1‑140 (MQ=255)

                                               caccaGAGAACATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAAcagc                                                                                     >  1:71340/1‑140 (MQ=255)

                                                    gagaACATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGTCt                                                                                <  1:1727413/140‑1 (MQ=255)

                                                    gagaACATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGTCt                                                                                >  1:767746/1‑140 (MQ=255)

                                                       aaCATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGTCTGCg                                                                             >  2:1255340/1‑140 (MQ=255)

                                                            gTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGTCTGCGTTTCa                                                                        >  1:1220688/1‑140 (MQ=255)

                                                            gTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGTCTGCGTTTCa                                                                        >  1:611253/1‑140 (MQ=255)

                                                              tAAACATCTCAATCATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGTCTGCGTTTCAGc                                                                      <  1:1045780/140‑1 (MQ=255)

                                                               aaaCATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGTCTGCGTTTCAGCt                                                                     <  1:1724679/140‑1 (MQ=255)

                                                                 aCATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGTCTGCGTTTCAGCTCg                                                                   <  1:131670/140‑1 (MQ=255)

                                                                 aCATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGCTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGTCTGCGTTTCAGCTCg                                                                   >  1:1721667/1‑140 (MQ=255)

                                                                   aTCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGTCTGCGTTTCAGCTCGcc                                                                 <  1:1043007/140‑1 (MQ=255)

                                                                    tctcAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGTCTGCGTTTCAGCTCGCCt                                                                <  2:641226/140‑1 (MQ=255)

                                                                     ctcAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGTCTGCGTTTCAGCTCGCCTa                                                               >  2:235357/1‑140 (MQ=255)

                                                                      tcAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGTCTGCGTTTCAGCTCGCCTaa                                                              >  2:359251/1‑140 (MQ=255)

                                                                      tcAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGTCTGCGTTTCAGCTCGCCTaa                                                              >  2:1413929/1‑140 (MQ=255)

                                                                      tcAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGTCTGCGTTTCAGCTCGCCTaa                                                              >  2:219600/1‑140 (MQ=255)

                                                                       cAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGTCTGCGTTTCAGCTCGCCTAAg                                                             >  1:685166/1‑140 (MQ=255)

                                                                       cAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGTCTGCGTTTCAGCTCGCCTAAg                                                             >  2:1382895/1‑140 (MQ=255)

                                                                             tgGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGTCTGCGTTTCAGCTCGCCTAAGCCACCt                                                       >  1:1728262/1‑140 (MQ=255)

                                                                                tGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGTCTGCGTTTCAGCTCGCCTAAGCCACCTTTg                                                    >  2:448728/1‑140 (MQ=255)

                                                                                  ccTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATGAGCGCACCGATAACAAACAGCAGTCTGCGTTTCAGCTCGCCTAAGCCACCTTTGGc                                                  >  2:1122302/1‑140 (MQ=255)

                                                                                  ccTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGTCTGCGTTTCAGCTCGCCTAAGCCACCTTTGGc                                                  >  2:894932/1‑140 (MQ=255)

                                                                                  ccTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGTCTGCGTTTCAGCTCGCCTAAGCCACCTTTGGc                                                  >  2:1612197/1‑140 (MQ=255)

                                                                                    tCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGTCTGCGTTTCAGCTCGCCTAAGCCACCTTTGGCAc                                                <  1:320721/140‑1 (MQ=255)

                                                                                     cGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGTCTGCGTTTCAGCTCGCCTAAGCCACCTTTGGCACt                                               <  2:1179381/140‑1 (MQ=255)

                                                                                     cGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGTCTGCGTTTCAGCTCGCCTAAGCCACCTTTGGCACt                                               <  1:144430/140‑1 (MQ=255)

                                                                                       cTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGTCTGCGTTTCAGCTCGCCTAAGCCACCTTTGGCACttt                                             >  1:763584/1‑140 (MQ=255)

                                                                                         gTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGTCTGCGTTTCAGCTCGCCTAAGCCACCTTTGGCACTTTGa                                           <  1:1159825/140‑1 (MQ=255)

                                                                                         gTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGTCTGCGTTTCAGCTCGCCTAAGCCACCTTTGGCACTTTGa                                           <  1:1188729/140‑1 (MQ=255)

                                                                                         gTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGTCTGCGTTTCAGCTCGCCTAAGCCACCTTTGGCACTTTGa                                           <  1:362152/140‑1 (MQ=255)

                                                                                         gTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGTCTGCGTTTCAGCTCGCCTAAGCCACCTTTGGCACTTTGa                                           <  1:539592/140‑1 (MQ=255)

                                                                                            gCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGTCTGCGTTTCAGCTCGCCTAAGCCACCTTTGGCACTTTGaaaa                                        >  1:1645926/1‑140 (MQ=255)

                                                                                              tCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGTCTGCGTTTCAGCTCGCCTAAGCCACCTTTGGCACTTTGAAAATc                                      >  2:1615342/1‑140 (MQ=255)

                                                                                               cAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGTCTGCGTTTCAGCTCGCCTAAGCCACCTTTGGCACTTTGAAAATCt                                     <  1:234691/140‑1 (MQ=255)

                                                                                                    aGTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGTCTGCGTTTCAGCTCGCCTAAGCCACCTTTGGCACTTTGAAAATCTAATcc                                <  1:894932/140‑1 (MQ=255)

                                                                                                     gTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGTCTGCGTTTCAGCTCGCCTAAGCCACCTTTGGCACTTTGAAAATCTAATccc                               <  1:1413929/140‑1 (MQ=255)

                                                                                                     gTTTGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGTCTGCGTTTCAGCTCGCCTAAGCCACCTTTGGCACTTTGAAAATCTAATccc                               <  1:19237/140‑1 (MQ=255)

                                                                                                       ttGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGTCTGCGTTTCAGCTCGCCTAAGCCACCTTTGGCACTTTGAAAATCTAATCCCgg                             <  2:169737/140‑1 (MQ=255)

                                                                                                       ttGGCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGTCTGCGTTTCAGCTCGCCTAAGCCACCTTTGGCACTTTGAAAATCTAATCCCgg                             <  1:234137/140‑1 (MQ=255)

                                                                                                          gCAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGTCTGCGTTTCAGCTCGCCTAAGCCACCTTTGGCACTTTGAAAATCTAATCCCGgttg                          >  1:807327/1‑140 (MQ=255)

                                                                                                           cAAGTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGCCTGCGTTTCAGCTCGCCTAAGCCACCTTTGGCACTTTGAAAATCTAATCCCGgttgt                         >  1:396812/1‑140 (MQ=255)

                                                                                                              gTACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGTCTGCGTTTCAGCTCGCCTAAGCCACCTTTGGCACTTTGAAAATCTAATCCCGGTTGTTTa                      >  1:766356/1‑140 (MQ=255)

                                                                                                               tACAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGTCTGCGTTTCAGCTCGCCTAAGCCACCTTTGGCACTTTGAAAATCTAATCCCGGTTGTTTAg                     <  2:337901/140‑1 (MQ=255)

                                                                                                                aCAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGTCTGCGTTTCAGCTCGCCTAAGCCACCTTTGGCACTTTGAAAATCTAATCCCGGTTGTTTAGc                    >  1:1150970/1‑140 (MQ=255)

                                                                                                                 cAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGTCTGCGTTTCAGCTCGCCTAAGCCACCTTTGGCACTTTGAAAATCTAATCCCGGTTGTTTAGcc                   <  1:1608031/140‑1 (MQ=255)

                                                                                                                 cAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGTCTGCGTTTCAGCTCGCCTAAGCCACCTTTGGCACTTTGAAAATCTAATCCCGGTTGTTTAGcc                   <  2:1573432/140‑1 (MQ=255)

                                                                                                                 cAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGTCTGCGTTTCAGCTCGCCTAAGCCACCTTTGGCACTTTGAAAATCTAATCCCGGTTGTTTAGcc                   <  1:641968/140‑1 (MQ=255)

                                                                                                                 cAGCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGTCTGCGTTTCAGCTCGCCTAAGCCACCTTTGGCACTTTGAAAATCTAATCCCGGTTGTTTAGcc                   <  1:1334351/140‑1 (MQ=255)

                                                                                                                   gCGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGTCTGCGTTTCAGCTCGCCTAAGCCACCTTTGGCACTTTGAAAATCTAATCCCGGTTGTTTAGCCAt                 <  1:1491902/140‑1 (MQ=255)

                                                                                                                    cGGCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGTCTGCGTTTCAGCTCGCCTAAGCCACCTTTGGCACTTTGAAAATCTAATCCCGGTTGTTTAGCCATc                >  1:1406003/1‑140 (MQ=255)

                                                                                                                     ggCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGTCTGCGTTTCAGCTCGCCTAAGCCACCTTTGGCACTTTGAAAATCTAATCCCGGTTGTTTAGCCATCt               <  1:751287/140‑1 (MQ=255)

                                                                                                                      gCATCAATACCAGGGTTCGGAATGAAAGAGCCAATACGGAACGCAATCAGCGCACCGATAACAAACAGCAGTCTGCGTTTCAGCTCGCCTAAGCCACCTTTGGCACTTTGAAAATCTAATCCCGGTTGTTTAGCCATCTg              <  2:192993/140‑1 (MQ=255)

                                                                                                                      gCATCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGTCTGCGTTTCAGCTCGCCTAAGCCACCTTTGGCACTTTGAAAATCTAATCCCGGTTGTTTAGCCATCTg              <  2:763584/140‑1 (MQ=255)

                                                                                                                        aTCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGTCTGCGTTTCAGCTCGCCTAAGCCACCTTTGGCACTTTGAAAATCTAATCCCGGTTGTTTAGCCATCTGCt            <  2:585922/140‑1 (MQ=255)

                                                                                                                         tCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGTCTGCGTTTCAGCTCGCCTAAGCCACCTTTGGCACTTTGAAAATCTAATCCCGGTTGTTTAGCCATCTGCTa           >  2:1508872/1‑140 (MQ=255)

                                                                                                                         tCAATACCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGTCTGCGTTTCAGCTCGCCTAAGCCACCTTTGGCACTTCGAAAATCTAATCCCGGTTGTTTAGCCATCTGCTa           >  1:857923/1‑140 (MQ=255)

                                                                                                                         tCAATACCAGGGCTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGTCTGCGTTTCAGCTCGCCTAAGCCACCTTTGGCACTTTGAAAATCTAATCCCGGTTGTTTAGCCATCTGCTa           >  2:159499/1‑140 (MQ=255)

                                                                                                                              aCCAGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGTCTGCGTTTCAGCTCGCCTAAGCCACCTTTGGCACTTTGAAAATCTAATCCCGGTTGTTTAGCCATATGCTACTTAt      >  2:880618/1‑140 (MQ=255)

                                                                                                                                 aGGGTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGTCTGCGTTTCAGCTCGCCTAAGCCACCTTTGGCACTTTGAAAATCTAATCCCGGTTGTTTAGCCATCTGCTACTTATTcc   >  1:1277316/1‑140 (MQ=255)

                                                                                                                                  gggTTCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGTCTGCGTTTCAGCTCGCCTAAGCCACCTTTGGCACTTTGAAAATCTAATCCCGGTTGTTTAGCCATCTGCTCCTTATTCCt  >  1:336549/1‑140 (MQ=255)

ATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGTACAGCGGCATCAATACCAGGGATCGGAATAAAAGAGCCAATACGGAACACAATCAGCGCACCGATAACAAACAGCAGTCTGCGTTTCAGCTCGCCTAAGCCACCTTTGGCACTTTGAAAATCTAATCCCGGTTGTTTAGCCATCTGCTACTTATTCCT  >  NC_000913/3443843‑3444112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A