Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A30 F60 I1 R1 10 43.1 1453344 99.0% 1438810 139.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
JC NC_000913 2,168,430 +ATGTTGTACAGAACATTTCCTACATAAGTAGGAA intergenic (‑124/‑282) yegR ← / → yegS uncharacterized protein YegR/lipid kinase

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 2168408 =4 (0.090)43 (1.090)
+CTGTACAACAT		 33/256 0.2 91.2% intergenic (‑102/‑304) yegR/yegS uncharacterized protein YegR/lipid kinase
?NC_000913 = 2168430 5 (0.120)intergenic (‑124/‑282) yegR/yegS uncharacterized protein YegR/lipid kinase

ATTTCAAAAAAGAGCATATACCTAATATTCAACTAAACAGTGGCATCTTCAATATAATATATTAAAGCCCCCATGGAGTTACCCTGAAGGGCCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/2168536‑2168408
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAAGGTGAGATGAAAAAGATAGC  <  NC_000913/2168430‑2168293
                                                                                                                                 |||||||||||                                                                                                                                          
ATTTCAAAAAAGAGCATATACCTAATATTCAACTAAACAGTGGCATCTTCAATATAATATATTAAAGCCCCCATGGAGTTACCCTGAAGGGCCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTCTGTACAACAT                                                                                                                                            >  1:58297/1‑140
                  TACCTAATATTCAACTAAACAGTGGCATCTTCAATATAATATATTAAAGCCCCCATGGAGTTACCCTGAAGGGCCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTCTGTACAACATTTCCTACTTATGTAGGAA                                                                                                                          <  2:73785/140‑1
                     CTAATATTCAACTAAACAGTGGCATCTTCAATATAATATATTAAAGCCCCCATGGAGTTACCCTGAAGGGCCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTCTGTACAACATTTCCTACTTATGTAGGAAATG                                                                                                                       <  1:339911/140‑1
                     CTAATATTCAACTAAACAGTGGCATCTTCAATATAATATATTAAAGCCCCCATGGAGTTACCCTGAAGGGCCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTCTGTACAACATTTCCTACTTATGTAGGAAATG                                                                                                                       <  1:685394/140‑1
                      TAATATTCAACTAAACAGTGGCATCTTCAATATAATATATTAAAGCCCCCATGGAGTTACCCTGAAGGGCCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTCTGTACAACATTTCCTACTTATGTAGGAAATGT                                                                                                                      >  1:38848/1‑140
                      TAATATTCAACTAAACAGTGGCATCTTCAATATAATATATTAAAGCCCCCATGGAGTTACCCTGAAGGGCCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTCTGTACAACATTTCCTACTTATGTAGGAAATGT                                                                                                                      >  1:632968/1‑140
                            TCAACTAAACAGTGGCATCTTCAATATAATATATTAAAGCCCCCATGGAGTTACCCTGAAGGGCCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTCTGTACAACATTTCCTACTTATGTAGGAAATGTTGTACA                                                                                                                <  1:520109/140‑1
                             CAACTAAACAGTGGCATCTTCAATATAATATATTAAAGCCTCCATGGAGTTACCCTGCAGGGCCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTCTGTACAACATTTCCTACTTATGTAGGAAATGTTGTACAG                                                                                                               <  1:404651/140‑1
                             CAACTAAACAGTGGCATCTTCAATATAATATATTAAAGCCCCCATGGAGTTACCCTGAAGGGCCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTCTGTACAACATTTCCTACTTATGTAGGAAATGTTGTACAG                                                                                                               <  2:502890/140‑1
                              AACTAAACAGTGGCATCTTCAATATAATATATTAAAGCCCCCATGGAGTTACCCTGAAGGGCCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTCTGTACAACATTTCCTACTTATGTAGGAAATGTTGTACAGA                                                                                                              >  2:240684/1‑140
                               ACTAAACAGTGGCATCTTCAATATAATATATTAAAGCCCCCATGGAGTTACCCTGAAGGGCCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTCTGTACAACATTTCCTACTTATGTAGGAAATGTTGTACAGAA                                                                                                             <  1:228645/140‑1
                               ACTAAACAGTGGCATCTTCAATATAATATATTAAAGCCCCCATGGAGTTACCCTGAAGGGCCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTCTGTACAACATTTCCTACTTATGTAGGAAATGTTGTACAGAA                                                                                                             >  1:593555/1‑140
                                   AACAGTGGCATCTTCAATATAATATATTAAAGCCCCCATGGAGTTACCCTGAAGGGCCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTCTGTACAACATTTCCTACTTATGTAGGAAATGTTGTACAGAACATT                                                                                                         >  2:601258/1‑140
                                   AACAGTGGCATCTTCAATATAATATATTAAAGCCCCCATGGAGTTACCCTGAAGGGCCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTCTGTACAACATTTCCTACTTATGTAGGAAATGTTGTACAGAACATT                                                                                                         >  1:371476/1‑140
                                        TGGCATCTTCAATATAATATATTAAAGCCCCCATGGAGTTACCCTGAAGGGCCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTCTGTACAACATTTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTA                                                                                                    <  2:562376/140‑1
                                          GCATCTTCAATATAATATATTAAAGCCCCCATGGAGTTACCCTGAAGGGCCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTCTGTACAACATTTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATA                                                                                                  >  1:634646/1‑140
                                             TCTTCAATATAATATATTAAAGCCCCCATGGAGTTACCCTGAAGGGCCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTCTGTACAACATTTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATC                                                                                               >  1:417400/1‑140
                                                  AATATAATATATTAAAGCCCCCATGGAGTTACCCTGAAGGGCCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTCTGTACAACATTTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATT                                                                                          >  1:272537/1‑140
                                                      TAATATATTAAAGCCCCCATGGAGTTACCCTGAAGGGCCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTCTGTACAACATTTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAAT                                                                                      <  1:525254/140‑1
                                                      TAATATATTAAAGCCCCCATGGAGTTACCCTGAAGGGCCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTCTGTACAACATTTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAAT                                                                                      <  2:184613/140‑1
                                                       AATATATTAAAGCCCCCATGGAGTTACCCTGAAGGGCCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTCTGTACAACATTTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATT                                                                                     >  2:329656/1‑140
                                                         TATATTAAAGCCCCCATGGAGTTACCCTGAAGGGCCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTCTGTACAACATTTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTAT                                                                                   >  1:693784/1‑140
                                                         TATATTAAAGCCCCCATGGAGTTACCCTGAAGGGCCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTCTGTACAACATTTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTAT                                                                                   >  2:64625/1‑140
                                                          ATATTAAAGCCCCCATGGAGTTACCCTGAAGGGCCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTCTGTACAACATTTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATA                                                                                  <  2:422179/140‑1
                                                              TAAAGCCCCCATGGAGTTACCCTGAAGGGCCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTCTGTACAACATTTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAA                                                                              >  2:103844/1‑140
                                                                AAGCCCCCATGGAGTTACCCTGAAGGGCCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTCTGTACAACATTTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATC                                                                            >  1:618093/1‑140
                                                                AAGCCCCCATGGAGTTACCCTGAAGGGCCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTCTGTACAACATTTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATC                                                                            >  1:551650/1‑140
                                                                 AGCCCCCATGGAGTTACCCTGAAGGGCCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTCTGTACAACATTTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCA                                                                           <  2:634646/140‑1
                                                                    CCCCATGGAGTTACCCTGAAGGGCCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTCTGTACAACATTTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGC                                                                        <  1:103844/140‑1
                                                                     CCCATGGAGTTACCCTGAAGGGCCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTCTGTACAACATTTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCC                                                                       >  1:2734/1‑140
                                                                     CCCATGGAGTTACCCTGAAGGGCCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTCTGTACAACATTTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCC                                                                       >  1:101401/1‑140
                                                                            AGTTACCCTGAAGGGCCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTCTGTACAACATTTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTA                                                                <  2:93738/140‑1
                                                                              TTACCCTGAAGGGCCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTCTGTACAACATTTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATA                                                              >  2:645611/1‑140
                                                                                      AAGGGCCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTCTGTACAACATTTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACA                                                      >  2:603002/1‑140
                                                                                      AAGGGCCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTCTGTACAACATTTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACA                                                      <  2:94000/140‑1
                                                                                      AAGGGCCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTCTGTACAACATTTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACA                                                      <  2:521797/140‑1
                                                                                           CCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTCTGTACAACATTTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGA                                                 >  2:156877/1‑140
                                                                                             TCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTCTGTACAACATTTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGA                                               <  2:2734/140‑1
                                                                                                   TCCGTAATTCCTACTTATGTAGGAAATGTTCTGTACAACATTTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCG                                         <  2:58297/140‑1
                                                                                                   TCCGTAATTCCTACTTATGTAGGAAATGTTCTGTACAACATTTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCG                                         <  2:38848/140‑1
                                                                                                    CCGTAATTCCTACTTATGTAGGAAATGTTCTGTACAACATTTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGT                                        <  2:693784/140‑1
                                                                                                             CTACTTATGTAGGAAATGTTCTGTACAACATTTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTA                               >  2:681369/1‑140
                                                                                                               ACTTATGTAGGAAATGTTCTGTACAACATTTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAAT                             <  1:431482/140‑1
                                                                                                                            attTTCTGTACAACATTTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAAGGTGAG                <  2:356913/137‑1
                                                                                                                                       AACATTTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAAGGTGAGATGAAAAAGAT     <  2:593555/140‑1
                                                                                                                                        ACATTTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAAGGTGAGATGAAAAAGATA    <  2:150957/140‑1
                                                                                                                                          ATTTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAAGGTGAGATGAAAAAGATAGC  <  2:417400/140‑1
                                                                                                                                 |||||||||||                                                                                                                                          
ATTTCAAAAAAGAGCATATACCTAATATTCAACTAAACAGTGGCATCTTCAATATAATATATTAAAGCCCCCATGGAGTTACCCTGAAGGGCCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/2168536‑2168408
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAAGGTGAGATGAAAAAGATAGC  <  NC_000913/2168430‑2168293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.

GATK/CNVnator alignment

N/A