Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F60 I1 R1 10 115.7 3938298 99.1% 3902853 139.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,947,372 G→A intergenic (‑194/‑15) mltA ← / → metZ membrane‑bound lytic murein transglycosylase A/tRNA‑Met

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,947,3720GA97.9% 328.3 / ‑1.0 95intergenic (‑194/‑15)mltA/metZmembrane‑bound lytic murein transglycosylase A/tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (40/53);  minor base . (1/1);  total (41/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAG  >  NC_000913/2947244‑2947508
                                                                                                                                 |                                                                                                                                        
tgaGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCTAGCGGAGTATAATGCGCATCCAc                                                                                                                               <  1:369576/140‑1 (MQ=255)
tgaGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCAc                                                                                                                               >  2:1223720/1‑140 (MQ=255)
tgaGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCAc                                                                                                                               <  1:1691058/140‑1 (MQ=255)
tgaGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCAc                                                                                                                               >  2:241671/1‑140 (MQ=255)
 gaGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACg                                                                                                                              <  1:1592961/140‑1 (MQ=255)
   gcagcCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGa                                                                                                                            >  1:20173/1‑140 (MQ=255)
    cagcCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGAc                                                                                                                           >  1:1008495/1‑140 (MQ=255)
    cagcCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGAc                                                                                                                           >  2:306937/1‑140 (MQ=255)
     agcCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGAcg                                                                                                                          <  2:1679854/140‑1 (MQ=255)
     agcCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGAcg                                                                                                                          <  1:1279354/140‑1 (MQ=255)
     agcCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGAcg                                                                                                                          <  1:1069015/140‑1 (MQ=255)
       ccTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGAcgcg                                                                                                                        >  1:969266/1‑140 (MQ=255)
         tctcCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGTAAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCgg                                                                                                                       >  1:1862094/1‑140 (MQ=255)
         tctcCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCggg                                                                                                                      >  1:1964773/1‑140 (MQ=255)
          ctcCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATACCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCgggg                                                                                                                     >  1:455920/1‑140 (MQ=255)
           tcCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGTAAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCgggg                                                                                                                     <  1:342662/140‑1 (MQ=255)
           tcCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGt                                                                                                                    >  1:1095258/1‑140 (MQ=255)
            cccTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATA‑TGCGCATCCACGGACGCGGGGTgg                                                                                                                  >  1:1633408/1‑140 (MQ=255)
             ccTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTgg                                                                                                                  <  2:989150/140‑1 (MQ=255)
               tGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGag                                                                                                                <  2:1964773/140‑1 (MQ=255)
               tGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGag                                                                                                                <  2:71669/140‑1 (MQ=255)
                taCGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGagc                                                                                                               <  1:961039/139‑1 (MQ=255)
                 aCGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGagca                                                                                                              >  2:1129857/1‑140 (MQ=255)
                 aCGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGagca                                                                                                              >  1:1544713/1‑140 (MQ=255)
                 aCGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGagca                                                                                                              >  2:1488548/1‑140 (MQ=255)
                 aCGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGagca                                                                                                              <  2:969266/140‑1 (MQ=255)
                 aCGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGagca                                                                                                              <  1:176051/140‑1 (MQ=255)
                  cGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGagcag                                                                                                             <  1:995235/140‑1 (MQ=255)
                     aaaTTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCt                                                                                                          >  1:1755607/1‑140 (MQ=255)
                     aaaTTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCt                                                                                                          >  1:1730472/1‑140 (MQ=255)
                             cacaAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAATGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCtc                                                                                                  >  1:165508/1‑140 (MQ=255)
                               caAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCtcgt                                                                                                <  2:916393/140‑1 (MQ=255)
                                 aaaaaTAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCtcgtcg                                                                                              >  1:1339265/1‑140 (MQ=255)
                                 aaaaaTAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCtcgtcg                                                                                              >  1:1570529/1‑140 (MQ=255)
                                 aaaaaTAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCtcgtcg                                                                                              <  2:1570529/140‑1 (MQ=255)
                                  aaaaTAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCgg                                                                                             <  2:1288257/140‑1 (MQ=255)
                                  aaaaTAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCgg                                                                                             <  1:962603/140‑1 (MQ=255)
                                   aaaTAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCggg                                                                                            <  1:1944039/140‑1 (MQ=255)
                                       aGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCa                                                                                        <  1:1474982/140‑1 (MQ=255)
                                        ggCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCAt                                                                                       <  2:558583/140‑1 (MQ=255)
                                        ggCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCAt                                                                                       <  2:892175/140‑1 (MQ=255)
                                            ttAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAAcc                                                                                   >  2:837613/1‑140 (MQ=255)
                                             tAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAAccc                                                                                  >  2:1954665/1‑140 (MQ=255)
                                              aGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCg                                                                                 >  2:1121949/1‑140 (MQ=255)
                                              aGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCg                                                                                 >  2:672321/1‑140 (MQ=255)
                                              aGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCg                                                                                 >  1:973259/1‑140 (MQ=255)
                                                  aTTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAgg                                                                             >  1:1535279/1‑140 (MQ=255)
                                                    ttgtttttgttCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGgtc                                                                           <  1:241671/140‑1 (MQ=255)
                                                      gtttttgttCAAAATCATGCCAAATCCGTGATCGGGGTAAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGgtcg                                                                          <  2:1862094/140‑1 (MQ=255)
                                                      gtttttgttCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGgtcgt                                                                         <  2:1755607/140‑1 (MQ=255)
                                                          ttgttCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATA‑TGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGtt                                                                    <  2:1633408/140‑1 (MQ=255)
                                                           tgttCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGtt                                                                    <  2:1339265/140‑1 (MQ=255)
                                                             ttCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCa                                                                  >  2:1466960/1‑140 (MQ=255)
                                                                 aaaTCATGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATc                                                              >  1:693040/1‑140 (MQ=255)
                                                                 aaaTCATGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATc                                                              <  2:817115/140‑1 (MQ=255)
                                                                   aTCATGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCg                                                            <  1:1290855/140‑1 (MQ=255)
                                                                    tCATGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCgg                                                           <  2:20173/140‑1 (MQ=255)
                                                                    tCATACCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCgg                                                           <  2:455920/140‑1 (MQ=255)
                                                                       tGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGccc                                                        >  2:49095/1‑140 (MQ=255)
                                                                       tGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGccc                                                        >  1:175390/1‑140 (MQ=255)
                                                                       tGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGACGGATCAAATCCTGccc                                                        >  2:1726183/1‑140 (MQ=255)
                                                                          cAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCg                                                     <  2:129567/140‑1 (MQ=255)
                                                                            aaTCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCa                                                   >  2:371347/1‑140 (MQ=255)
                                                                              tCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAAc                                                 >  2:715358/1‑140 (MQ=255)
                                                                              tCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAAc                                                 <  2:192506/140‑1 (MQ=255)
                                                                              tCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAAc                                                 <  1:1954665/140‑1 (MQ=255)
                                                                              tCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAAc                                                 <  1:323764/140‑1 (MQ=255)
                                                                              tCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAAc                                                 <  1:371347/140‑1 (MQ=255)
                                                                                      cGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTaaa                                         >  1:1056831/1‑140 (MQ=255)
                                                                                       ggcgT‑AAAAAAAAGGTTGCATGAAAACGCGATCGGAGTATAATGGGCATACACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCaaat                                        <  2:1526482/137‑2 (MQ=18)
                                                                                        gggT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAAt                                       >  2:1692191/1‑140 (MQ=255)
                                                                                           t‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTtg                                    <  2:303807/140‑1 (MQ=255)
                                                                                             aaaaaaaaGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTtga                                   <  1:965792/140‑1 (MQ=255)
                                                                                             aaaaaaaaGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTtga                                   <  2:1008495/140‑1 (MQ=255)
                                                                                              aaaaaaaGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTtgat                                  >  2:1717476/1‑140 (MQ=255)
                                                                                               aaaaaaGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTtgatg                                 <  2:1095258/140‑1 (MQ=255)
                                                                                                  aaaGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAg                              <  1:1121949/140‑1 (MQ=255)
                                                                                                  aaaGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAg                              <  1:315353/140‑1 (MQ=255)
                                                                                                  aaaGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGACCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAg                              <  1:1081113/140‑1 (MQ=255)
                                                                                                    aGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCGCCCGCAACCAATTAAAATTTGATGAAGTa                            >  2:838602/1‑140 (MQ=255)
                                                                                                    aGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTattgaa                                      >  2:1378215/1‑125 (MQ=255)
                                                                                                    aGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTattgaa                                      <  1:1378215/130‑6 (MQ=255)
                                                                                                    aGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAATTTTGATGAAGTa                            >  1:1526482/1‑140 (MQ=255)
                                                                                                    aGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAgaa                            >  2:1189645/1‑138 (MQ=255)
                                                                                                    aGGTTGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTa                            >  2:1870427/1‑140 (MQ=255)
                                                                                                       ttGCATGAAAATGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAg                         <  2:165508/140‑1 (MQ=255)
                                                                                                       ttGCATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAg                         <  2:175390/140‑1 (MQ=255)
                                                                                                          cATGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGAAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAATTTGGATGAGGTAAAGCAg                      >  2:1508070/1‑140 (MQ=18)
                                                                                                            tGAAAACGCGAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTa                    <  2:973259/140‑1 (MQ=255)
                                                                                                                    cgAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGAcg            <  2:1085150/140‑1 (MQ=255)
                                                                                                                    cgAGCGGAGTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGAcg            <  1:1692191/140‑1 (MQ=255)
                                                                                                                            gTATAATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTgg    <  1:1717476/140‑1 (MQ=255)
                                                                                                                              ataATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGag  <  1:1870427/140‑1 (MQ=255)
                                                                                                                              ataATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGag  <  1:1223720/140‑1 (MQ=255)
                                                                                                                              ataATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGag  <  1:1466960/140‑1 (MQ=255)
                                                                                                                              ataATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGag  <  1:1488548/140‑1 (MQ=255)
                                                                                                                              ataATGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGag  <  2:1535279/140‑1 (MQ=255)
                                                                                                                                 |                                                                                                                                        
TGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAG  >  NC_000913/2947244‑2947508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A