Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F60 I1 R1	13	156.7	5387192	99.4%	5354868	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,504,535	G→A	V162I (GTC→ATC)	frlR →	putative DNA‑binding transcriptional regulator FrlR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,504,535	0	G	A	99.3%	520.6 / ‑4.6	148	V162I (GTC→ATC)	frlR	putative DNA‑binding transcriptional regulator FrlR
Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (79/67); total (81/67)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.62e-01

Only 100 of 149 total aligned reads displayed.

GCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGACCATCGATATCTTTGCCGCCACCCGCCCGCAGGCAAAATGGCTGAAATGCGAACTGGGCGAACCG  >  NC_000913/3504401‑3504669

ttgAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCgaa                                                                                                                                   <  2:2679831/138‑1 (MQ=255)

gCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCgaa                                                                                                                                   <  2:1921702/140‑1 (MQ=255)

 cGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCgaag                                                                                                                                  >  2:2576038/1‑140 (MQ=255)

 cGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCgaag                                                                                                                                  <  1:1559754/140‑1 (MQ=255)

  gAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACAACgaagg                                                                                                                                 <  1:2004725/140‑1 (MQ=255)

   aaaaGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCgaagga                                                                                                                                <  2:808562/140‑1 (MQ=255)

     aaGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCgaaggaag                                                                                                                              <  1:999128/140‑1 (MQ=255)

      aGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGc                                                                                                                             <  1:1241823/140‑1 (MQ=255)

      aGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGc                                                                                                                             >  1:1841953/1‑140 (MQ=255)

       gCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCt                                                                                                                            <  2:2378055/140‑1 (MQ=255)

       gCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCt                                                                                                                            <  1:2569170/140‑1 (MQ=255)

        cTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTc                                                                                                                           <  1:1149199/140‑1 (MQ=255)

        cTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTc                                                                                                                           <  2:1070723/140‑1 (MQ=255)

        cTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTc                                                                                                                           <  2:752564/140‑1 (MQ=255)

        cTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTc                                                                                                                           <  1:2129892/140‑1 (MQ=255)

         tGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTcc                                                                                                                          <  2:287262/140‑1 (MQ=255)

           aaCATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCAc                                                                                                                        >  2:2409823/1‑140 (MQ=255)

              aTCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTa                                                                                                                     <  2:1946925/140‑1 (MQ=255)

                ccccGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATc                                                                                                                   >  1:1656223/1‑140 (MQ=255)

                ccccGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATc                                                                                                                   >  2:1320312/1‑140 (MQ=255)

                 cccGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCa                                                                                                                  <  1:426050/140‑1 (MQ=255)

                  ccGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAg                                                                                                                 >  2:2041381/1‑140 (MQ=255)

                   cGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGt                                                                                                                <  2:653757/140‑1 (MQ=255)

                   cGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGt                                                                                                                <  1:50938/140‑1 (MQ=255)

                   cGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGt                                                                                                                <  2:1715831/140‑1 (MQ=255)

                   cGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGt                                                                                                                <  2:1525086/140‑1 (MQ=255)

                   cGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGt                                                                                                                <  1:2226745/140‑1 (MQ=255)

                        aCAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTTGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTAttt                                                                                                           >  1:1769473/1‑140 (MQ=255)

                        aCAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTAttt                                                                                                           <  1:1931264/140‑1 (MQ=255)

                        aCAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTAttt                                                                                                           <  1:1507837/140‑1 (MQ=255)

                          aGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCa                                                                                                         >  1:2442874/1‑140 (MQ=255)

                            cGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAgg                                                                                                       <  2:2096578/140‑1 (MQ=255)

                             gAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGa                                                                                                      <  1:800589/140‑1 (MQ=255)

                             gAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGa                                                                                                      <  1:1870153/140‑1 (MQ=255)

                             gAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGa                                                                                                      <  1:2670731/140‑1 (MQ=255)

                                      cATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGa                                                                                             <  2:1699171/140‑1 (MQ=255)

                                        tctcTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGaca                                                                                           <  1:942190/140‑1 (MQ=255)

                                        tctcTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGaca                                                                                           <  1:2409823/140‑1 (MQ=255)

                                           ctGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACAcgc                                                                                        >  2:1413572/1‑140 (MQ=255)

                                           ctGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACAcgc                                                                                        >  1:2073796/1‑140 (MQ=255)

                                           ctGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACAcgc                                                                                        >  1:2402223/1‑140 (MQ=255)

                                             gCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGa                                                                                      <  2:4954/140‑1 (MQ=255)

                                             gCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGa                                                                                      >  1:1586008/1‑140 (MQ=255)

                                              ccGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAg                                                                                     >  1:326727/1‑140 (MQ=255)

                                              ccGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAg                                                                                     >  1:819258/1‑140 (MQ=255)

                                                 ggTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTgg                                                                                  >  2:1300925/1‑140 (MQ=255)

                                                          tctcGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACa                                                                         >  1:2461242/1‑140 (MQ=255)

                                                           ctcGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACaa                                                                        >  2:2521440/1‑140 (MQ=255)

                                                             cGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACaaaa                                                                      >  2:1400712/1‑140 (MQ=255)

                                                               aTAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAg                                                                    >  2:1223155/1‑140 (MQ=255)

                                                               aTAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAg                                                                    >  2:639230/1‑140 (MQ=255)

                                                               aTAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAg                                                                    >  1:1048987/1‑140 (MQ=255)

                                                                tAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGa                                                                   <  1:1351884/140‑1 (MQ=255)

                                                                tAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGa                                                                   <  1:1320312/140‑1 (MQ=255)

                                                                  aaGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGAcc                                                                 >  2:1824899/1‑140 (MQ=255)

                                                                  aaGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGAcc                                                                 <  1:323407/140‑1 (MQ=255)

                                                                     agCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGACCATc                                                              >  2:387171/1‑140 (MQ=255)

                                                                         gCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGACCATCGata                                                          >  1:1290806/1‑140 (MQ=255)

                                                                              ttATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGACCATCGATATCttt                                                     >  1:867996/1‑140 (MQ=255)

                                                                                    aTAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACTTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGACCATCGATATCTTTgccgcc                                               >  1:128507/1‑140 (MQ=255)

                                                                                    aTAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGCTTTGACACGCGAGTGGTCAGCGACAAAAAGACCATCGATATCTTTgccgcc                                               >  1:1958937/1‑140 (MQ=255)

                                                                                       gctCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGACCATCGATATCTTTGCCGCCAcc                                            >  2:733581/3‑140 (MQ=255)

                                                                                       gTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGACCATCGATATCTTTGCCGCCAcc                                            >  1:283613/1‑140 (MQ=255)

                                                                                       gTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGACCATCGATATCTTTGCCGCCAcc                                            >  1:2609592/1‑140 (MQ=255)

                                                                                       gTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGACCATCGATATCTTTGCCGCCAcc                                            >  2:1458451/1‑140 (MQ=255)

                                                                                       gTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGACCATCGATATCTTTGCCGCCAcc                                            >  1:1551748/1‑140 (MQ=255)

                                                                                       gTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGACCATCGATATCTTTGCCGCCAcc                                            >  2:1990117/1‑140 (MQ=255)

                                                                                       gTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGACCATCGATATCTTTGCCGCCAcc                                            >  1:727624/1‑140 (MQ=255)

                                                                                       gTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGACCATCGATATCTTTGCCGCCAcc                                            >  1:1687131/1‑140 (MQ=255)

                                                                                       gTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGACCATCGATATCTTTGCCGCCAcc                                            >  2:562305/1‑140 (MQ=255)

                                                                                        ttCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACTTCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGACCATCGATATCTTTGCCGCCAccc                                           >  2:1244980/1‑140 (MQ=255)

                                                                                          cATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGACCATCGATATCTTTGCCGCCAcccgc                                         >  2:1233076/1‑140 (MQ=255)

                                                                                            tGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGACCATCGATATCTTTGCCGCCAcccgccc                                       >  2:918393/1‑140 (MQ=255)

                                                                                            tGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGACCATCGATATCTTTGCCGCCAcccgccc                                       <  1:1437881/140‑1 (MQ=255)

                                                                                             ggATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGACCATCGATATCTTTGCCGCCAcccgcccg                                      >  1:199350/1‑140 (MQ=255)

                                                                                               aTCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGACCATCGATATCTATGCCGCCACCCGCCCGCa                                    <  1:19589/140‑1 (MQ=255)

                                                                                                tCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGACCATCGATATCTTTGCCGCCACCCGCCCGCAg                                   >  1:2615741/1‑140 (MQ=255)

                                                                                                  ccGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGACCATCGATATCTTTGCCGCCACCCGCCCGCAGGc                                 >  2:5751/1‑140 (MQ=255)

                                                                                                  ccGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGACCATCGATATCTTTGCCGCCACCCGCCCGCAGGc                                 >  2:947588/1‑140 (MQ=255)

                                                                                                  ccGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGACCATCGATATCTTTGCCGCCACCCGCCCGCAGGc                                 >  1:65267/1‑140 (MQ=255)

                                                                                                  ccGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGACCATCGATATCTTTGCCGCCACCCGCCCGCAGGc                                 >  1:678297/1‑140 (MQ=255)

                                                                                                  ccGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGACCATCGATATCTTTGCCGCCACCCGCCCGCAGGc                                 >  1:894293/1‑140 (MQ=255)

                                                                                                   cGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGACCATCGATATCTTTGCCGCCACCCGCCCGCAGGCa                                >  1:1634021/1‑140 (MQ=255)

                                                                                                   cGCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGAAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGACCATCGATATCTTTGCCGCCACCCGCCCGCAGGCa                                >  1:178936/1‑140 (MQ=255)

                                                                                                    gCTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGACCATCGATATCTTTGCCGCCACCCGCCCGCAGGCaa                               <  1:2521440/140‑1 (MQ=255)

                                                                                                     cTGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGACCATCGATATCTTTGCCGCCACCCGCCCGCAGGCaaa                              >  2:2112018/1‑140 (MQ=255)

                                                                                                      tGTCGCGTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGACCATCGATATCTTTGCCGCCACCCGCCCGCAGGCaaaa                             <  2:1881641/140‑1 (MQ=255)

                                                                                                         cgcgTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGACCATCGATATCTTTGCCGCCACCCGCCCGCAGGCAAAATgg                          >  2:344350/1‑140 (MQ=255)

                                                                                                           cgTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGACCATCGATATCTTTGCCGCCACCCGCCCGCAGGCAAAATGGCt                        <  2:1048987/140‑1 (MQ=255)

                                                                                                           cgTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGACCATCGATATCTTTGCCGCCACCCGCCCGCAGGCAAAATGGCt                        <  2:1551748/140‑1 (MQ=255)

                                                                                                           cgTTATCCTGACTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGACCATCGATATCTTTGCCGCCACCCGCCCGCAGGCAAAATGGCt                        <  2:1841953/140‑1 (MQ=255)

                                                                                                                     aCTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGACCATCGATATCTTTGCCGCCACCCGCCCGCAGGCAAAATGGCTGAAATGCGaa              >  2:1476679/1‑140 (MQ=255)

                                                                                                                      cTTTGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGACCATCGATATCTTTGCCGCCACCCGCCCGCAGGCAAAATGGCTGAAATGCGAAc             >  1:2385006/1‑140 (MQ=255)

                                                                                                                       tttGATGAGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGACCATCGATATCTTTGCCGCCACCCGCCCGCAGGCAAAATGGCTGAAATGCGAACt            >  2:2246987/1‑140 (MQ=255)

                                                                                                                         tgatgaGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGACCATCGATATCTTTGCCGCCACCCGCCCGCAGGCAAAATGGCTGAAATGCGAACTgg          <  2:1769473/140‑1 (MQ=255)

                                                                                                                          gatgaGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGACCATCGATATCTTTGCCGCCACCCGCCCGCAGGCAAAATGGCTGAAATGCGAACTggg         >  1:574349/1‑140 (MQ=255)

                                                                                                                          gatgaGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGACCATCGATATCTTTGCCGCCACCCGCCCGCAGGCAAAATGGCTGAAATGCGAACTggg         >  2:856728/1‑140 (MQ=255)

                                                                                                                          gatgaGATTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGACCATCGATATCTTTGCCGCCACCCGCCCGCAGGCAAAATGGCTGAAATGCGAACTggg         >  2:2233695/1‑140 (MQ=255)

                                                                                                                             gagaTTTACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGACCATCGATATCTTTGCCGCCACCCGCCCGCAGGCAAAATGGCTGAAATGCGAACTGGGCGa      <  1:1904358/140‑1 (MQ=255)

                                                                                                                                 tttACATCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGACCATCGATATCTTTGCCGCCACCCGCCCGCAGGCAAAATGGCTGAAATGCGAACTGGGCGAACCg  >  2:414230/1‑140 (MQ=255)

GCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGACCATCGATATCTTTGCCGCCACCCGCCCGCAGGCAAAATGGCTGAAATGCGAACTGGGCGAACCG  >  NC_000913/3504401‑3504669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A