Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A23 F60 I1 R1 20 97.1 3292108 99.2% 3265771 138.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,276,404 C→A G35G (GGC→GGA)  yejF → putative oligopeptide ABC transporter ATP binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,276,4040CA100.0% 377.2 / NA 107G35G (GGC→GGA) yejFputative oligopeptide ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (58/49);  total (58/49)

Only 100 of 108 total aligned reads displayed.

GCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCGCTGCT  >  NC_000913/2276272‑2276535
                                                                                                                                    |                                                                                                                                   
      gATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAAcgctggcgc                                                                                                                        <  1:899648/140‑1 (MQ=255)
      gATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAAcgctggcgc                                                                                                                        <  2:1153771/140‑1 (MQ=255)
       aTCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAAcgctggcgct                                                                                                                       >  2:208096/1‑140 (MQ=255)
        tCCTAATAAGGCGGTGTGGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAAcgctggcgctg                                                                                                                      <  1:1380866/140‑1 (MQ=255)
         ccTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAAcgctggcgctgg                                                                                                                     <  1:208096/140‑1 (MQ=255)
           taataaGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAAcgctggcgc                                                                                                                        >  2:1309111/1‑135 (MQ=255)
           taataaGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAAcgctggcgc                                                                                                                        <  1:1309111/135‑1 (MQ=255)
           taataaGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCtggtg                                                                                                                   >  2:1082409/1‑140 (MQ=255)
            aataaGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCtggtgg                                                                                                                  <  2:1217875/140‑1 (MQ=255)
             ataaGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTggtggg                                                                                                                 <  2:1405902/140‑1 (MQ=255)
              taaGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTggtgggt                                                                                                                <  1:80847/140‑1 (MQ=255)
              taaGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTggtgggt                                                                                                                >  2:1507052/1‑140 (MQ=255)
              taaGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTggtgggt                                                                                                                <  1:1077572/140‑1 (MQ=255)
              taaGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTggtgggt                                                                                                                <  1:1174580/140‑1 (MQ=255)
                  gCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGt                                                                                                            >  2:1494717/1‑140 (MQ=255)
                  gCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGt                                                                                                            <  2:1167735/140‑1 (MQ=255)
                     gtgtAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAg                                                                                                         >  1:1084976/1‑140 (MQ=255)
                      tgtAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCtggtg                                                                                                                   >  1:704121/1‑129 (MQ=255)
                      tgtAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCtggtg                                                                                                                   <  2:704121/129‑1 (MQ=255)
                      tgtAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAgg                                                                                                        >  1:1349666/1‑140 (MQ=255)
                       gtAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGt                                                                                                       >  1:362831/1‑140 (MQ=255)
                         aGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTc                                                                                                     <  2:1084976/140‑1 (MQ=255)
                             tGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGc                                                                                                 <  1:1141401/140‑1 (MQ=255)
                               aCGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGc                                                                                                 <  2:603552/138‑1 (MQ=255)
                               aCGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGc                                                                                                 >  1:603552/1‑138 (MQ=255)
                               aCGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCaa                                                                                               >  2:1159428/1‑140 (MQ=255)
                                cGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGTCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCaaa                                                                                              >  1:139177/1‑140 (MQ=255)
                                cGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCaaa                                                                                              >  2:1048848/1‑140 (MQ=255)
                                cGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCaaa                                                                                              >  1:160130/1‑140 (MQ=255)
                                cGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCaaa                                                                                              >  2:1457119/1‑140 (MQ=255)
                                cGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCaaa                                                                                              >  1:409373/1‑140 (MQ=255)
                                cGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCaa                                                                                               >  2:13038/1‑139 (MQ=255)
                                cGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCaa                                                                                               <  1:13038/139‑1 (MQ=255)
                                cGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGGTCATGCaaa                                                                                              >  1:949733/1‑140 (MQ=255)
                                    aaCTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCg                                                                                          >  1:356556/1‑140 (MQ=255)
                                         tGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTAcc                                                                                     >  2:368353/1‑140 (MQ=255)
                                           ttAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCa                                                                                                    <  2:1561360/123‑1 (MQ=255)
                                           ttAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCa                                                                                                    >  1:1561360/1‑123 (MQ=255)
                                            tAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACcgcg                                                                                  <  1:1082409/140‑1 (MQ=255)
                                             aGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCgcgc                                                                                 <  1:1457119/140‑1 (MQ=255)
                                               cGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTg                                                                               >  1:288824/1‑140 (MQ=255)
                                                 aTTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTc                                                                             >  1:134839/1‑140 (MQ=255)
                                                 aTTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTc                                                                             >  1:432023/1‑140 (MQ=255)
                                                   tGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCaa                                                                           >  2:865709/1‑140 (MQ=255)
                                                      aaaTTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAAttt                                                                        <  1:1048848/140‑1 (MQ=255)
                                                      aaaTTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAAttt                                                                        <  1:674067/140‑1 (MQ=255)
                                                           tGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGc                                                                   <  1:445724/140‑1 (MQ=255)
                                                           tGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGc                                                                   <  2:874957/140‑1 (MQ=255)
                                                            gTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGcc                                                                  <  2:529006/140‑1 (MQ=255)
                                                            gTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGcc                                                                  <  1:1507052/140‑1 (MQ=255)
                                                            gTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGcc                                                                  <  2:1194129/140‑1 (MQ=255)
                                                            gTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGcc                                                                  <  2:482933/140‑1 (MQ=255)
                                                            gTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGc                                                                   >  1:729864/1‑139 (MQ=255)
                                                            gTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGc                                                                   <  2:729864/139‑1 (MQ=255)
                                                                    gTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCt                                                          >  2:93656/1‑140 (MQ=255)
                                                                          gCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCccg                                                    <  2:160130/140‑1 (MQ=255)
                                                                            cATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCccgcc                                                  <  2:409373/140‑1 (MQ=255)
                                                                             aTCAGCAACCCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCccgc                                                   <  1:702745/138‑1 (MQ=255)
                                                                             aTCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCccgc                                                   >  2:702745/1‑138 (MQ=255)
                                                                              tCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCgg                                                <  2:139177/140‑1 (MQ=255)
                                                                              tCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCgg                                                >  1:113337/1‑140 (MQ=255)
                                                                              tCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGCCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCgg                                                <  2:949733/140‑1 (MQ=255)
                                                                               cAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCccgc                                                   <  1:146945/136‑1 (MQ=255)
                                                                               cAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCccgc                                                   >  2:146945/1‑136 (MQ=255)
                                                                                 gCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTg                                             <  2:113337/140‑1 (MQ=255)
                                                                                     aCCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAata                                         >  2:901633/1‑140 (MQ=255)
                                                                                     aCCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAata                                         >  1:1224198/1‑140 (MQ=255)
                                                                                         tacgtacAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCgg                                                >  2:637697/1‑129 (MQ=255)
                                                                                         tacgtacAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCgg                                                <  1:637697/129‑1 (MQ=255)
                                                                                          acgtacAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATAtctct                                    >  1:182168/1‑140 (MQ=255)
                                                                                                 gtagtCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGa                             >  1:1188521/1‑140 (MQ=255)
                                                                                                  tagtCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGat                            <  1:752108/140‑1 (MQ=255)
                                                                                                   agtCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGata                           <  1:865709/140‑1 (MQ=255)
                                                                                                   agtCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGata                           >  2:1300044/1‑140 (MQ=255)
                                                                                                    gtCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTTTCCGGCGatat                          >  2:773647/1‑140 (MQ=255)
                                                                                                    gtCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGatat                          >  1:866469/1‑140 (MQ=255)
                                                                                                     tCAATGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATAtt                         >  2:754114/1‑140 (MQ=255)
                                                                                                       aaTGATGTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCg                       >  2:213386/1‑140 (MQ=255)
                                                                                                          gatgTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGttt                    <  2:362831/140‑1 (MQ=255)
                                                                                                            tgTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGa                             <  1:1174197/129‑1 (MQ=255)
                                                                                                            tgTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGa                             >  2:1174197/1‑129 (MQ=255)
                                                                                                            tgTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTc                  >  2:503493/1‑140 (MQ=255)
                                                                                                            tgTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTc                  >  2:196674/1‑140 (MQ=255)
                                                                                                            tgTTTCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTc                  >  1:1074921/1‑140 (MQ=255)
                                                                                                              tttCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCa                 <  1:145349/139‑1 (MQ=255)
                                                                                                              tttCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCa                 >  2:145349/1‑139 (MQ=255)
                                                                                                              tttCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCAt                >  2:134105/1‑140 (MQ=255)
                                                                                                              tttCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCAt                >  1:283513/1‑140 (MQ=255)
                                                                                                               ttCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATg               >  1:129185/1‑140 (MQ=255)
                                                                                                                tCACTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATgg              <  1:134105/140‑1 (MQ=255)
                                                                                                                  aCTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCg            <  2:866469/140‑1 (MQ=255)
                                                                                                                   cTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGa           >  1:434070/1‑140 (MQ=255)
                                                                                                                   cTACAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGa           >  2:934245/1‑140 (MQ=255)
                                                                                                                      cAGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGGTTTCATGGCGAATc        >  1:758249/1‑140 (MQ=255)
                                                                                                                       aGATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCg       >  2:1638745/1‑140 (MQ=255)
                                                                                                                        gATTGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCgc      >  1:1432458/1‑140 (MQ=255)
                                                                                                                           tGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCgctgc   >  2:1061380/1‑140 (MQ=255)
                                                                                                                           tGAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCgctgc   >  2:1193392/1‑140 (MQ=255)
                                                                                                                            gAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCgctgct  >  1:1028352/1‑140 (MQ=255)
                                                                                                                            gAGGCTGGAGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCgctgct  <  1:1193392/140‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                   
GCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCGCTGCT  >  NC_000913/2276272‑2276535

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A