Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A44 F60 I1 R1 18 142.5 4932706 99.3% 4898177 138.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,280,436 A→C A16A (GCT→GCG)  rsuA ← 16S rRNA pseudouridine(516) synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,280,4360AC100.0% 727.7 / NA 184A16A (GCT→GCG) rsuA16S rRNA pseudouridine(516) synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (87/97);  total (87/97)

Only 100 of 185 total aligned reads displayed.

AACGTGGACCGTGTTGCTGCGCCAGCGGGTTGCCATCGTAAGCGACATCATGTTCAGGAAGCAGTTTGAACGCTGCATTACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATAGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGAGGTCGGGCATTGACCCGAAAATCTGAACTGTTGTCTGCCCAGTATAGCGGTCTAATAACAT  >  NC_000913/2280300‑2280571
                                                                                                                                        |                                                                                                                                       
aaCGTGGACCGTGTTGCTGCGCCAGCGGGTTGCCATCGTAAGCGACATCATGTTCAGGAAGCAGTTTGAACGCTGCATTACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCa                                                                                                                                      >  1:358503/1‑140 (MQ=255)
aaCGTGGACCGTGTTGCTGCGCCAGCGGGTTGCCATCGTAAGCGACATCATGTTCAGGAAGCAGTTTGAACGCTGCATTACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCa                                                                                                                                      <  1:1657406/140‑1 (MQ=255)
 aCGTGGACCGTGTTGCTGCGCCAGCGGGTTGCCATCGTAAGCGACATCATGTTCAGGAAGCAGTTTGAACGCTGCA‑TACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACg                                                                                                                                    >  2:638011/1‑140 (MQ=255)
  cGTGGACCGTGTTGCTGCGCCAGCGGGTTGCCATCGTAAGCGACATCATGTTCAGGAAGCAGTTTGAACGCTGCATTACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACg                                                                                                                                    >  1:1791950/1‑140 (MQ=255)
   gTGGACCGTGTTGCTGCGCCAGCGGGTTGCCATCGTAAGCGACATCATGTTCAGGAAGCAGTTTGAACGCTGCATTACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACgg                                                                                                                                   <  2:358503/140‑1 (MQ=255)
    tGGACCGTGTTGCTGCGCCAGCGGGTTGCCATCGTAAGCGACATCATGTTCAGGAAGCAGTTTGAACGCTGCATTACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGc                                                                                                                                  <  2:1837036/140‑1 (MQ=255)
      gACCGTGTTGCTGCGCCAGCGGGTTGCCATCGTAAGCGACATCATGTTCAGGAAGCAGTTTGAACGCTGCATTACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTa                                                                                                                                >  2:1728351/1‑140 (MQ=255)
      gACCGTGTTGCTGCGCCAGCGGGTTGCCATCGTAAGCGACATCATGTTCAGGAAGCAGTTTGAACGCTGCATTACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTa                                                                                                                                >  2:1719290/1‑140 (MQ=255)
      gACCGTGTTGCTGCGCCAGCGGGTTGCCATCGTAAGCGACATCATGTTCAGGAAGCAGTTTGAACGCTGCATTACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTa                                                                                                                                >  1:935112/1‑140 (MQ=255)
       aCCGTGTTGCTGCGCCAGCGGGTTGCCATCGTAAGCGACATCATGTTCAGGAAGCAGTTTGAACGCTGCATTACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTaa                                                                                                                               >  2:760523/1‑140 (MQ=255)
       aCCGTGTTGCTGCGCCAGCGGGTTGCCATCGTAAGCGACATCATGTTCAGGAAGCAGTTTGAACGCTGCATTACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTaa                                                                                                                               >  2:169768/1‑140 (MQ=255)
         cGTGTTGCTGCGCCAGCGGGTTGCCATCGTAAGCGACATCATGTTCAGGAAGCAGTTTGAACGCTGCATTACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACg                                                                                                                             <  1:51625/140‑1 (MQ=255)
         cGTGTTGCTGCGCCAGCGGGTTGCCATCGTAAGCGACATCATGTTCAGGAAGCAGTTTGAACGCTGCATTACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACg                                                                                                                             >  1:1302163/1‑140 (MQ=255)
         cGTGTTGCTGCGCCAGCGGGTTGCCATCGTAAGCGACATCATGTTCAGGAAGCAGTTTGAACGCTGCATTACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACg                                                                                                                             <  2:1710187/140‑1 (MQ=255)
          gtgtTGCTGCGCCAGCGGGTTGCCATCGTAAGCGACATCATGTTCAGGAAGCAGTTTGAACGCTGCATTACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGc                                                                                                                            >  1:2419206/1‑140 (MQ=255)
           tgtTGCTGCGCCAGCGGGTTGCCATCGTAAGCGACATCATGTTCAGGAAGCAGTTTGAACGCTGCATTACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGcc                                                                                                                           >  1:1939703/1‑140 (MQ=255)
            gtTGCTGCGCCAGCGGGTTGCCATCGTAAGCGACATCATGTTCAGGAAGCAGTTTGAACGCTGCATTACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCg                                                                                                                          <  2:393357/140‑1 (MQ=255)
                 tgcGCCAGCGGGTTGCCATCGTAAGCGACATCATGTTCAGGAAGCAGTTTGAACGCTGCATTACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTg                                                                                                                     <  2:1732961/140‑1 (MQ=255)
                  gcgcCAGCGGGTTGCCATCGTAAGCGACATCATGTTCAGGAAGCAGTTTGAACGCTGCATTACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGc                                                                                                                    <  1:1821238/140‑1 (MQ=255)
                     ccAGCGGGTTGCCATCGTAAGCGACATCATGTTCAGGAAGCAGTTTGAACGCTGCATTACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCtgt                                                                                                                 >  2:1144055/1‑140 (MQ=255)
                     ccAGCGGGTTGCCATCGTAAGCGACATCATGTTCAGGAAGCAGTTTGAACGCTGCATTACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCtgt                                                                                                                 >  1:861556/1‑140 (MQ=255)
                            gTTGCCATCGTAAGCGACATCATGTTCAGGAAGCAGTTTGAACGCTGCATTACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATaa                                                                                                          <  2:72554/140‑1 (MQ=255)
                             ttGCCATCGTAAGCGACATCATGTTCAGGAAGCAGTTTGAACGCTGCATTACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATaaa                                                                                                         >  2:23063/1‑140 (MQ=255)
                               gCCATCGTAAGCGACATCATGTTCAGGAAGCAGTTTGAACGCTGCATTACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAAtt                                                                                                       >  2:306523/1‑140 (MQ=255)
                                 cATCGTAAGCGACATCATGTTCAGGAAGCAGTTTGAACGCTGCA‑TACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTAt                                                                                                    <  1:638011/140‑1 (MQ=255)
                                  aTCGTAAGCGACATCATGTTCAGGAAGCAGTTTGAACGCTGCATTACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTAt                                                                                                    <  1:1249081/140‑1 (MQ=255)
                                  aTCGTAAGCGACATCATGTTCAGGAAGCAGTTTGAACGCTGCATTACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTAt                                                                                                    <  1:1728351/140‑1 (MQ=255)
                                    cGTAAGCGACATCATGTTCAGGAAGCAGTTTGAACGCTGCATTACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCa                                                                                                  <  1:1089236/140‑1 (MQ=255)
                                      tAAGCGACATCATGTTCAGGAAGCAGTTTGAACGCTGCATTACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAg                                                                                                <  1:2163718/140‑1 (MQ=255)
                                          cGACATCATGTTCAGGAAGCAGTTTGAACGCTGCATTACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGc                                                                                            >  2:154945/1‑140 (MQ=255)
                                          cGACATCATGTTCAGGAAGCAGTTTGAACGCTGCATTACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGc                                                                                            <  1:2080098/140‑1 (MQ=255)
                                          cGACATCATGTTCAGGAAGCAGTTTGAACGCTGCATTACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGc                                                                                            <  2:861556/140‑1 (MQ=255)
                                           gACATCATGTTCAGGAAGCAGTTTGAACGCTGCATTACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCa                                                                                           <  2:2226104/140‑1 (MQ=255)
                                           gACATCATGTTCAGGAAGCAGTTTGAACGCTGCATTACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCa                                                                                           <  2:2328207/140‑1 (MQ=255)
                                             catcatGTTCAGGAAGCAGTTTGAACGCTGCATTACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCAtg                                                                                         >  1:1811879/1‑140 (MQ=255)
                                              atcatGTTCAGGAAGCAGTTTGAACGCTGTATTACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGc                                                                                            <  1:777399/136‑1 (MQ=255)
                                              atcatGTTCAGGAAGCAGTTTGAACGCTGCATTACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGc                                                                                            >  2:2064509/1‑136 (MQ=255)
                                              atcatGTTCAGGAAGCAGTTTGAACGCTGCATTACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCAt                                                                                          <  2:1913600/138‑1 (MQ=255)
                                                catGTTCAGGAAGCAGTTTGAACGCTGCATTACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCAtgtgt                                                                                      <  2:1801504/140‑1 (MQ=255)
                                                 atGTTCAGGAAGCAGTTTGAACGCTGCATTACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCAtgtgtg                                                                                     >  1:545720/1‑140 (MQ=255)
                                                      cAGGAAGCAGTTTGAACGCTGCATTACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGc                                                                                            <  2:1250050/128‑1 (MQ=255)
                                                      cAGGAAGCAGTTTGAACGCTGCATTACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGAtt                                                                                >  2:10969/1‑140 (MQ=255)
                                                       aGGAAGCAGTTTGAACGCTGCATTACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGAttt                                                                               >  1:2302301/1‑140 (MQ=255)
                                                        ggAAGCAGTTCGAACGCTGCATTACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGAtttt                                                                              >  1:1888042/1‑140 (MQ=255)
                                                         gAAGCAGTTTGAACGCTGCATTACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTg                                                                             >  1:628183/1‑140 (MQ=255)
                                                         gAAGCAGTTTGAACGCTGCATTACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTg                                                                             >  2:766680/1‑140 (MQ=255)
                                                                     aCGCTGCATTACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACg                                                                 <  2:2419206/140‑1 (MQ=255)
                                                                      cGCTGCATTACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACgg                                                                <  1:2296312/140‑1 (MQ=255)
                                                                      cGCTGCATTACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACgg                                                                <  1:154945/140‑1 (MQ=255)
                                                                        cTGCATTACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGAg                                                              >  2:83807/1‑140 (MQ=255)
                                                                         tGCATTACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGAgg                                                             >  1:1039430/1‑140 (MQ=255)
                                                                         tGCATTACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGAgg                                                             >  2:40942/1‑140 (MQ=255)
                                                                         tGCATTACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGAgg                                                             >  2:1351260/1‑140 (MQ=255)
                                                                          gCATTACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGcc                                                                           >  2:2030602/1‑125 (MQ=255)
                                                                          gCATTACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGAGGt                                                            <  1:1719290/140‑1 (MQ=255)
                                                                           cATTACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGAGGTc                                                           >  1:1410498/1‑140 (MQ=255)
                                                                           cATTACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGAGGTc                                                           <  2:935112/140‑1 (MQ=255)
                                                                                   aCGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACgg                                                                <  1:1496998/127‑1 (MQ=255)
                                                                                   aCGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACgg                                                                >  2:1496998/1‑127 (MQ=255)
                                                                                   aCGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGAGGTCGGGCATTg                                                   >  1:1462753/1‑140 (MQ=255)
                                                                                   aCGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGAGGTCGGGCATTg                                                   >  1:818254/1‑140 (MQ=255)
                                                                                   aCGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGAGGTCGGGCATTg                                                   >  1:1017825/1‑140 (MQ=255)
                                                                                     gATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGAGGTCGGGCATTGAc                                                 >  2:587871/1‑140 (MQ=255)
                                                                                     gATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGAGGTCGGGCATTGAc                                                 >  1:936598/1‑140 (MQ=255)
                                                                                     gATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGAGGTCGGGCATTGAc                                                 >  1:1767104/1‑140 (MQ=255)
                                                                                     gATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGAGGTCGGGCATTGAc                                                 >  1:214955/1‑140 (MQ=255)
                                                                                        ttCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGAGGTCGGGCATTGACCCg                                              <  2:1410498/140‑1 (MQ=255)
                                                                                             cATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGAGGTCGGGCATTGACCCGAAAAt                                         <  1:40942/140‑1 (MQ=255)
                                                                                               tCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGAGGTCGGGCATTGACCCGAAAATCt                                       >  1:204593/1‑140 (MQ=255)
                                                                                                 gACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGAGGTCGGGCATTGACCCg                                              >  2:2367105/1‑131 (MQ=255)
                                                                                                 gACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGAGGTCGGGCATTGACCCg                                              <  1:2367105/131‑1 (MQ=255)
                                                                                                 gACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGAGGTCGGGCATTGACCCGAAAATCTGa                                     >  1:1400503/1‑140 (MQ=255)
                                                                                                    ggTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGAGGTCGGGCATTGACCCGAAAATCTGAACt                                  <  2:818254/140‑1 (MQ=255)
                                                                                                     gTGACACGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGTAGGTCGGGCATTGACCCg                                              >  2:1661474/1‑127 (MQ=255)
                                                                                                         cacGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGAGGTCGGGCATTGACCCGAAAATCTGAACtgttgt                             >  2:451255/1‑140 (MQ=255)
                                                                                                         cacGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGAGGTCGGGCATTGACCCGAAAATCTGAACtgttgt                             <  2:1302163/140‑1 (MQ=255)
                                                                                                         cacGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGAGGTCGGGCATTGACCCGAAAATCTGAACtgttgt                             <  2:1939703/140‑1 (MQ=255)
                                                                                                           cGATTGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGAGGTCGGGCATTGACCCGAAAATCTGAACTGTTGTCt                           <  1:451255/140‑1 (MQ=255)
                                                                                                              ttGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGAGGTCGGGCATTGACCCGAAAATCt                                       >  2:1618538/1‑125 (MQ=255)
                                                                                                              ttGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGAGGTCGGGCATTGACCCGAAAATCt                                       <  1:1618538/125‑1 (MQ=255)
                                                                                                              ttGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGAGGTCGGGCATTGACCCGAAAATCTGAACTGTTGTCTGcc                        <  2:628183/140‑1 (MQ=255)
                                                                                                              ttGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGAGGTCGGGCATTGACCCGAAAATCTGAACTGTTGTCTGcc                        <  2:214955/140‑1 (MQ=255)
                                                                                                              ttGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGAGGTCGGGCATTGACCCGAAAATCTGAACTGTTGTCTGcc                        <  1:1314743/140‑1 (MQ=255)
                                                                                                              ttGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGAGGTCGGGCATTGACCCGAAAATCTGAACTGTTGTCTGcc                        <  1:977065/140‑1 (MQ=255)
                                                                                                              ttGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGAGGTCGGGCATTGACCCGAAAATCTGAACTGTTGTCTGcc                        <  2:1585845/140‑1 (MQ=255)
                                                                                                              ttGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGAGGTCGGGCATTGACCCGAAAATCTGAACTGTTGTCTGcc                        <  2:936598/140‑1 (MQ=255)
                                                                                                              ttGCCGCGGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCGGTAAAAACGGAGGTCGGGCATTGACCCGAAAATCTGAACTGTTGTCTGcc                        >  1:1077703/1‑140 (MQ=255)
                                                                                                                  cgcgGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGAGGTCGGGCATTGACCCGAAAATCTGAACTGTTGTCTGCCCAGt                    >  2:1227883/1‑140 (MQ=255)
                                                                                                                    cgGATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGAGGTCGGGCATTGACCCGAAAATCTGAACTGTTGTCTGCCCAGtat                  >  2:1934489/1‑140 (MQ=255)
                                                                                                                      gATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGAGGTCGGGCATTGACCCGAAAATCTGAACTGTTGTCTGCCCAGTATAg                <  1:169397/140‑1 (MQ=255)
                                                                                                                      gATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGAGGTCGGGCATTGACCCGAAAATCTGAACTGTTGTCTGCCCAGTATAg                <  1:2240018/140‑1 (MQ=255)
                                                                                                                      gATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGAGGTCGGGCATTGACCCGAAAATCTGAACTGTTGTCTGCCCAGTATAg                <  1:310537/140‑1 (MQ=255)
                                                                                                                      gATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGAGGTCGGGCATTGACCCGAAAATCTGAACTGTTGTCTGCCCAGTATAg                <  2:1811879/140‑1 (MQ=255)
                                                                                                                      gATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGAGGTCGGGCATTGACCCGAAAATCTGAACTGTTGTCTGCCCAGTATAg                <  1:1144055/140‑1 (MQ=255)
                                                                                                                      gATTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGAGGTCGGGCATTGACCCGAAAATCTGAACTGTTGTCTGCCCAGTATAg                <  2:1400503/140‑1 (MQ=255)
                                                                                                                       aTTTCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGAGGTCGGGCATTGACCCGAAAATCTGAACTGTTGTCTGCCCAGTATAGc               <  2:1462753/140‑1 (MQ=255)
                                                                                                                        tttCACGCCCGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGAGGTCGGGCATTGACCCGAAAATCTGAACTGTTGTCTGCCCAGTATAGCg              <  2:2464680/140‑1 (MQ=255)
                                                                                                                                 cGGCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGAGGTCGGGCATTGACCCGAAAATCTGAACTGTTGTCTGCCCAGTATAGCGGTCtaataa     >  2:1079495/1‑140 (MQ=255)
                                                                                                                                   gCAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGAGGTCGGGCATTGACCCGAAAATCTGAACTGTTGTCTGCCCAGTATAGCGGTCTAATAACa   <  2:1767104/140‑1 (MQ=255)
                                                                                                                                    cAATCGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGAGGTCGGGCATTGACCCGAAAATCTGAACTGTTGTCTGCCCAGTATAGCGGTCTAATAACAt  <  1:71456/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                       
AACGTGGACCGTGTTGCTGCGCCAGCGGGTTGCCATCGTAAGCGACATCATGTTCAGGAAGCAGTTTGAACGCTGCATTACGGACGATTTCGCCATCGACGGTGACACGATTGCCGCGGATTTCACGCCCGGCAATAGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGAGGTCGGGCATTGACCCGAAAATCTGAACTGTTGTCTGCCCAGTATAGCGGTCTAATAACAT  >  NC_000913/2280300‑2280571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A