Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A23 F60 I1 R1	20	97.1	3292108	99.2%	3265771	138.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,428,974	T→C	E422G (GAA→GGA)	purF ←	amidophosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,428,974	0	T	C	100.0%	415.0 / NA	105	E422G (GAA→GGA)	purF	amidophosphoribosyltransferase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (45/60); total (45/60)

Only 100 of 105 total aligned reads displayed.

GACGTAGACGCCGTTGAACACCGAGCATTCAAACTGCTGGATATCCGGATTTTCAGCGCGAACGGCGTCGATCAGATCGTTCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTTCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCAATACCATAAACGTTCGGGAAGCGAATTTCCGGTGCCGCAGAAGCGAGGTACACTTTCTTCGCTCCGGCTTCGCGTGCCATCTCGATAATCTG  >  NC_000913/2428838‑2429107

gACGTAGACGCCGTTGAACACCGAGCATTCAAACTGCTGGATATCCGGATTTTCAGCGCGAACGGCGTCGATCAGATCGTTCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCcgc                                                                                                                                    >  2:816520/1‑140 (MQ=255)

 aCGTAGACGCCGTTGAACACCGAGCATTCAAACTGCTGGATATCCGGATTTTCAGCGCGAACGGCGTCGATCAGATCGTTCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCcgcg                                                                                                                                   >  2:30658/1‑140 (MQ=255)

   gTAGACGCCGTTGAACACCGAGCATTCAAACTGCTGGATATCCGGATTTTCAGCGCGAACGGCGTCGATCAGATCGTTCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGAc                                                                                                                                 <  2:929266/140‑1 (MQ=255)

   gTAGACGCCGTTGAACACCGAGCATTCAAACTGCTGGATATCCGGATTTTCAGCGCGAACGGCGTCGATCAGATCGTTCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGAc                                                                                                                                 <  2:763753/140‑1 (MQ=255)

   gTAGACGCCGTTGAACACCGAGCATTCAAACTGCTGGATATCCGGATTTTCAGCGCGAACGGCGTCGATCAGATCGTTCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGAc                                                                                                                                 <  1:404117/140‑1 (MQ=255)

     aGACGCCGTTGAACACCGAGCATTCAAACTGCTGGATATCCGGATTTTCAGCGCGAACGGCGTCGATCAGATCGTTCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCg                                                                                                                               <  2:871342/140‑1 (MQ=255)

      gACGCCGTTGAACACCGAGCATTCAAACTGCTGGATATCCGGATTTTCAGCGCGAACGGCGTCGATCAGATCGTTCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGt                                                                                                                              <  1:1158568/140‑1 (MQ=255)

      gACGCCGTTGAACACCGAGCATTCAAACTGCTGGATATCCGGATTTTCAGCGCGAACGGCGTCGATCAGATCGTTCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGt                                                                                                                              <  2:692877/140‑1 (MQ=255)

        cGCCGTTGAACACCGAGCATTCAAACTGCTGGATATCCGGATTTTCAGCGCGAACGGCGTCGATCAGATCGTTCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGa                                                                                                                            <  1:801937/140‑1 (MQ=255)

           cGTTGAACACCGAGCATTCAAACTGCTGGATATCCGGATTTTCAGCGCGAACGGCGTCGATCAGATCGTTCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGt                                                                                                                              <  2:715743/135‑1 (MQ=255)

           cGTTGAACACCGAGCATTCAAACTGCTGGATATCCGGATTTTCAGCGCGAACGGCGTCGATCAGATCGTTCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGt                                                                                                                              >  1:715743/1‑135 (MQ=255)

           cGTTGAACACCGAGCATTCAAACTGCTGGATATCCGGATTTTCAGCGCGAACGGCGTCGATCAGATCGTTCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCg                                                                                                                         >  1:697724/1‑140 (MQ=255)

           cGTTGAACACCGAGCATTCAAACTGCTGGATATCCGGATTTTCAGCGCGAACGGCGTCGATCAGATCGTTCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCg                                                                                                                         >  1:1448506/1‑140 (MQ=255)

           cGTTGAACACCGAGCATTCAAACTGCTGGATATCCGGATTTTCAGCGCGAACGGCGTCGATCAGATCGTTCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCg                                                                                                                         <  2:755140/140‑1 (MQ=255)

             ttGAACACCGAGCATTCAAACTGCTGGATATCCGGATTTTCAGCGCGAACGGCGTCGATCAGATCGTTCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGAt                                                                                                                       <  1:383290/140‑1 (MQ=255)

             ttGAACACCGAGCATTCAAACTGCTGGATATCCGGATTTTCAGCGCGAACGGCGTCGATCAGATCGTTCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGAt                                                                                                                       >  2:26884/1‑140 (MQ=255)

             ttGAACACCGAGCATTCAAACTGCTGGATATCCGGATTTTCAGCGCGAACGGCGTCGATCAGATCGTTCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGAt                                                                                                                       <  1:30658/140‑1 (MQ=255)

              tGAACACCGAGCATTCAAACTGCTGGATATCCGGATTTTCAGCGCGAACGGCGTCGATCAGATCGTTCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGAc                                                                                                                                 >  2:402765/1‑129 (MQ=255)

              tGAACACCGAGCATTCAAACTGCTGGATATCCGGATTTTCAGCGCGAACGGCGTCGATCAGATCGTTCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGAc                                                                                                                                 <  1:402765/129‑1 (MQ=255)

                   acCGAGCATTCAAACTGCTGGATATCCGGATTTTCAGCGCGAACGGCGTCGATCAGATCGTTCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGAt                                                                                                                       <  2:582962/134‑1 (MQ=255)

                   acCGAGCATTCAAACTGCTGGATATCCGGATTTTCAGCGCGAACGGCGTCGATCAGATCGTTCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGAt                                                                                                                       >  1:582962/1‑134 (MQ=255)

                     cGAGCATTCAAACTGCTGGATATCCGGATTTTCAGCGCGAACGGCGTCGATCAGATCGTTCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCg                                                                                                               <  1:168668/140‑1 (MQ=255)

                     cGAGCATTCAAACTGCTGGATATCCGGATTTTCAGCGCGAACGGCGTCGATCAGATCGTTCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCg                                                                                                               >  1:399190/1‑140 (MQ=255)

                     cGAGCATTCAAACTGCTGGATATCCGGATTTTCAGCGCGAACGGCGTCGATCAGATCGTTCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCg                                                                                                               >  1:1292426/1‑140 (MQ=255)

                       aGCATTCAAACTGCTGGATATCCGGATTTTCAGCGCGAACGGCGTCGATCAGATCGTTCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGAt                                                                                                                       <  1:21836/130‑1 (MQ=255)

                       aGCATTCAAACTGCTGGATATCCGGATTTTCAGCGCGAACGGCGTCGATCAGATCGTTCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGAt                                                                                                                       >  2:21836/1‑130 (MQ=255)

                       aGCATTCAAACTGCTGGATATCCGGATTTTCAGCGCGAACGGCGTCGATCAGATCGTTCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTg                                                                                                             >  2:1166782/1‑140 (MQ=255)

                          aTTCAAACTGCTGGATATCCGGATTTTCAGCGCGAACGGCGTCGATCAGATCGTTCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGgcg                                                                                                          >  1:512655/1‑140 (MQ=255)

                            tCAAACTGCTGGATATCCGGATTTTCAGCGCGAACGGCGTCGATCAGATCGTTCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCt                                                                                                        <  1:905816/140‑1 (MQ=255)

                            tCAAACTGCTGGATATCCGGATTTTCAGCGCGAACGGCGTCGATCAGATCGTTCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCt                                                                                                        <  1:26884/140‑1 (MQ=255)

                            tCAAACTGCTGGATATCCGGATTTTCAGCGCGAACGGCGTCGATCAGATCGTTCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCt                                                                                                        <  1:1493759/140‑1 (MQ=255)

                             cAAACTGCTGGATATCCGGATTTTCAGCGCGAACGGCGTCGATCAGATCGTTCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTc                                                                                                       <  1:190530/140‑1 (MQ=255)

                             cAAACTGCTGGATATCCGGATTTTCAGCGCGAACGGCGTCGATCAGATCGTTCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTc                                                                                                       <  1:664923/140‑1 (MQ=255)

                               aaCTGCTGGATATCCGGATTTTCAGCGCGAACGGCGTCGATCAGATCGTTCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCgg                                                                                                     <  1:1257542/140‑1 (MQ=255)

                               aaCTGCTGGATATCCGGATTTTCAGCGCGAACGGCGTCGATCAGATCGTTCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCgg                                                                                                     <  2:1266161/140‑1 (MQ=255)

                               aaCTGCTGGATATCCGGATTTTCAGCGCGAACGGCGTCGATCAGATCGTTCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCgg                                                                                                     <  1:547795/140‑1 (MQ=255)

                               aaCTGCTGGATATCCGGATTTTCAGCGCGAACGGCGTCGATCAGATCGTTCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCgg                                                                                                     <  1:816520/140‑1 (MQ=255)

                               aaCTGCTGGATATCCGGATTTTCAGCGCGAACGGCGTCGATCAGATCGTTCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCgg                                                                                                     <  1:1368628/140‑1 (MQ=255)

                               aaCTGCTGGATATCCGGATTTTCAGCGCGAACGGCGTCGATCAGATCGTTCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCgg                                                                                                     <  1:671210/140‑1 (MQ=255)

                               aaCTGCTGGATATCCGGATTTTCAGCGCGAACGGCGTCGATCAGATCGTTCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCgg                                                                                                     <  1:386423/140‑1 (MQ=255)

                                    ctgGATATCCGGATTTTCAGCGCGAACGGCGTCGATCAGATCGTTCAGATCCTGGAAAATCAACCCGTTAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCatat                                                                                                >  2:1061104/1‑140 (MQ=255)

                                      ggATATCCGGATTTTCAGCGCGAACGGCGTCGATCAGATCGTTCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCa                                                                                              >  1:392566/1‑140 (MQ=255)

                                       gATATCCGGATTTTCAGTGCGAACGGCGTCGATCAGATCGTTCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCaa                                                                                             <  1:1467029/140‑1 (MQ=255)

                                       gATATCCGGATTTTCAGCGCGAACGGCGTCGATCAGATCGTTCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCaa                                                                                             <  2:1559402/140‑1 (MQ=255)

                                         tatCCGGATTTTCAGCGCGAACGGCGTCGATCAGATCGTTCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCAATa                                                                                           <  2:1292426/140‑1 (MQ=255)

                                                 ttttCAGCGCGAACGGCGTCGATCAGATCGTTCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCAATACCATAAAc                                                                                   >  1:283575/1‑140 (MQ=255)

                                                  tttCAGCGCGAACGGCGTCGATCAGATCGTTCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCAATACCATAAACg                                                                                  >  1:1617361/1‑140 (MQ=255)

                                                      aGCGCGAACGGCGTCGATCAGATCGTTCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCAATACCATAAACGTTCg                                                                              >  1:691741/1‑140 (MQ=255)

                                                        cgcgAACGGCGTCGATCAGATCGTTCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCAATACCATAAACGTTCggg                                                                            <  2:283575/140‑1 (MQ=255)

                                                         gcgAACGGCGTCGATCAGATCGTTCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCAATACCATAAACGTTCGGGa                                                                           >  1:12863/1‑140 (MQ=255)

                                                                      aTCAGATCGTTCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCAATACCATAAACGTTCGGGAAg                                                                         <  1:552414/129‑1 (MQ=255)

                                                                      aTCAGATCGTTCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCAATACCATAAACGTTCGGGAAg                                                                         >  2:552414/1‑129 (MQ=255)

                                                                      aTCAGATCGTTCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCAATACCATAAACGTTCGGGAAGCGAATTTCCgg                                                              >  1:1297111/1‑140 (MQ=255)

                                                                      aTCAGATCGTTCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCAATACCATAAACGTTCGGGAAGCGAATTTCCgg                                                              >  1:1408774/1‑140 (MQ=255)

                                                                        cAGATCGTTCAGATCCTGGAAAATCAACCCGTTAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCAATACCATAAACGTTCGGGAAGCGAATTTCCGGTg                                                            <  1:1061104/140‑1 (MQ=255)

                                                                          gATCGTTCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCAATACCATAAACGTTCGGGAAGCGAATTTCCGGTGcc                                                          >  1:263661/1‑140 (MQ=255)

                                                                           aTCGTTCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCAATACCATAAACGTTCGGGAAGCGAATTTCCGGTGCCg                                                         >  2:701650/1‑140 (MQ=255)

                                                                               ttCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCAATACCATAAACGTTCGGGAAGCGAATTTCCGGTGCCGCAGa                                                     >  1:1458899/1‑140 (MQ=255)

                                                                                tCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCAATACCATAAACGTTCGGGAAGCGAATTTCCGGTGCCGCAGaa                                                    <  1:1166782/140‑1 (MQ=255)

                                                                                tCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCAATACCATAAACGTTCGGGAAGCGAATTTCCGGTGCCGCAGaa                                                    <  2:1471010/140‑1 (MQ=255)

                                                                                tCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCAATACCATAAACGTTCGGGAAGCGAATTTCCGGTGCCGCAGaa                                                    <  2:1617361/140‑1 (MQ=255)

                                                                                tCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCAATACCATAAACGTTCGGGAAGCGAATTTCCGGTGCCGCAGaa                                                    >  1:470125/1‑140 (MQ=255)

                                                                                tCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCAATACCATAAACGTTCGGGAAGCGAATTTCCGGTGCCGCAGaa                                                    >  1:1471010/1‑140 (MQ=255)

                                                                                tCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCAATACCATAAACGTTCGGGAAGCGAATTTCCGGTGCCGCAGaa                                                    >  1:227854/1‑140 (MQ=255)

                                                                                tCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCAATACCATAAACGTTCGGGAAGCGAATTTCCGGTGCCGCAGaa                                                    >  1:218827/1‑140 (MQ=255)

                                                                                    aTCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCAATACCATAAACGTTCGGGAAGCGAATTTCCGGTGCCGCAGAAGCGa                                                >  1:95245/1‑140 (MQ=255)

                                                                                     tCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCAATACCATAAACGTTCGGGAAGCGAATTTCCGGTGCCGCAGAAGCGAg                                               >  1:1386407/1‑140 (MQ=255)

                                                                                      ccTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCAACAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCAATACCATAAACGTTCGGGAAGCGAATTTCCGGTGCCGCAGAAGCGAgg                                              <  2:1463474/140‑1 (MQ=255)

                                                                                       cTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCAATACCATAAACGTTCGGGAAGCGAATTTCCGGTGCCGCAGAAGCGAGGt                                             <  2:691741/140‑1 (MQ=255)

                                                                                       cTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCAATACCATAAACGTTCGGGAAGCGAATTTCCGGTGCCGCAGAAGCGAGGt                                             <  1:701650/140‑1 (MQ=255)

                                                                                         ggAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCAATACCATAAACGTTCGGGAAGCGAATTTCCGGTGCCGCAGaa                                                    <  1:1057788/131‑1 (MQ=255)

                                                                                         ggAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCAATACCATAAACGTTCGGGAAGCGAATTTCCGGTGCCGCAGAAGCGAGGTac                                           >  2:1641930/1‑140 (MQ=255)

                                                                                         ggAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCAATACCATAAACGTTCGGGAAGCGAATTTCCGGTGCCGCAGAAGCGAGGTac                                           >  2:900353/1‑140 (MQ=255)

                                                                                         ggAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCAATACCATAAACGTCCGGGAAGCGAATTTCCGGTGCCGCAGaa                                                    >  2:1057788/1‑131 (MQ=255)

                                                                                              aTCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCAATACCATAAACGTTCGGGAAGCGAATTTCCGGTGCCGCAGaa                                                    >  1:649578/1‑126 (MQ=255)

                                                                                              aTCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCAATACCATAAACGTTCGGGAAGCGAATTTCCGGTGCCGCAGaa                                                    >  1:731985/1‑126 (MQ=255)

                                                                                              aTCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCAATACCATAAACGTTCGGGAAGCGAATTTCCGGTGCCGCAGaa                                                    <  2:731985/126‑1 (MQ=255)

                                                                                              aTCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCAATACCATAAACGTTCGGGAAGCGAATTTCCGGTGCCGCAGaa                                                    <  2:649578/126‑1 (MQ=255)

                                                                                                 aaCCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCAATACCATAAACGTTCGGGAAGCGAATTTCCGGTGCCGCAGAAGCGAGGTACACTttctt                                   <  2:1458899/140‑1 (MQ=255)

                                                                                                    ccGTCAGCACCGATGATCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCAATACCATAAACGTTCGGGAAGCGAATTTCCGGTGCCGCAGAAGCGAGGTACACTTTCTTCGc                                >  1:1139488/1‑140 (MQ=255)

                                                                                                                atgatCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCAATACCATAAACGTTCGGGAAGCGAATTTCCGGTGCCGCAGAAGCGAGGTACACTTTCTTCGCTCCGGCTTcgcg                    >  1:1003269/1‑140 (MQ=255)

                                                                                                                atgatCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCAATACCATAAACGTTCAGGAAGCGAATTTCCGGTGCCGCAGAAGCGAGGTACACTTTCTTCGCTCCGGCTTcgcg                    >  2:703817/1‑140 (MQ=255)

                                                                                                                 tgatCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCAATACCATAAACGTTCGGGAAGCGAATTTCCGGTGCCGCAGAAGCGAGGTACACTTTCTTCGCTCCGGCTTCGCGt                   <  1:1484084/140‑1 (MQ=255)

                                                                                                                 tgatCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCAATACCATAAACGTTCGGGAAGCGAATTTCCGGTGCCGCAGAAGCGAGGTACACTTTCTTCGCTCCGGCTTCGCGt                   <  2:218827/140‑1 (MQ=255)

                                                                                                                    tCTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCAATACCATAAACGTTCGGGAAGCGAATTTCCGGTGCCGCAGAAGCGAGGTACACTTTCTTCGCTCCTGCTTCGCGTGcc                <  2:697724/140‑1 (MQ=255)

                                                                                                                     cTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCAATTCCATAAACGTTCGGGAAGCGAATTTCCGGTGCCGCAGAAGCGAGGTACACTTTCTTCGCTCCGGCTTCGCGTGCCa               <  1:1605761/140‑1 (MQ=255)

                                                                                                                     cTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCAATACCATAAACGTTCGGGAAGCGAATTTCCGGTGCCGCAGAAGCGAGGTACACTTTCTTCGCTCCGGCTTCGCGTGCCa               <  2:1386407/140‑1 (MQ=255)

                                                                                                                     cTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCAATACCATAAACGTTCGGGAAGCGAATTTCCGGTGCCGCAGAAGCGAGGTACACTTTCTTCGCTCCGGCTTCGCGTGCCa               <  2:227854/140‑1 (MQ=255)

                                                                                                                     cTGGCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCAATACCATAAACGTTCGGGAAGCGAATTTCCGGTGCCGCAGAAGCGAGGTACACTTTCTTCGCTCCGGCTTCGCGTGCCa               <  2:1003269/140‑1 (MQ=255)

                                                                                                                        gCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCAATACCATAAACGTTCGGGAAGCGAATTTCCGGTGCCGCAGAAGCGAGGTACACTTTCTTCGCTCCGGCTTCGCGTGcc                >  2:927812/1‑136 (MQ=255)

                                                                                                                        gCGAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCAATACCATAAACGTTCGGGAAGCGAATTTCCGGTGCCGCAGAAGCGAGGTACACTTTCTTCGCTCCGGCTTCGCGTGcc                <  1:927812/136‑1 (MQ=255)

                                                                                                                          gAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCAATACCATAAACGTTCGGGAAGCGAATTTCCGGTGCCGCAGAAGCGAGGTACACTTTCTTCGCTCCGGCTTCGCGTGCCATCTCg          >  2:699591/1‑140 (MQ=255)

                                                                                                                          gAATTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCAATACCATAAACGTTCGGGAAGCGAATTTCCGGTGCCGCAGAAGCGAGGTACACTTTCTTCGCTCCGGCTTCGCGTGCCATCTCg          <  2:512655/140‑1 (MQ=255)

                                                                                                                           aaTTTCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCAATACCATAAACGTTCGGGAAGCGAATTTCCGGTGCCGCAGAAGCGAGGTACACTTTCTTCGCTCCGGCTTCGCGTGCCATCTCGa         >  1:1287268/1‑140 (MQ=255)

                                                                                                                             tttCATCAACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCAATACCATAAACGTTCGGGAAGCGAATTTCCGGTGCCGCAGAAGCGAGGTACACTTTCTTCGCTCCGGCTTCGCGTGCCATCTCGATa       <  1:1641930/140‑1 (MQ=255)

                                                                                                                               tcatcaACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCAATACCATAAACGTTCGGGAAGCGAATTTCCGGTGCCGCAGAAGCGAGGTACACTTTCTTCGCTCCGGCTTCGCGTGCCATCTCGATAAt     <  2:1594693/140‑1 (MQ=255)

                                                                                                                               tcatcaACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCAATACCATAAACGTTCGGGAAGCGAATTTCCGGTGCCGCAGAAGCGAGGTACACTTTCTTCGCTCCGGCTTCGCGTGCCATCTCGATAAt     <  2:12863/140‑1 (MQ=255)

                                                                                                                               tcatcaACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCAATACCATAAACGTTCGGGAAGCGAATTTCCGGTGCCGCAGAAGCGAGGTACACTTTCTTCGCTCCGGCTTCGCATGCCATCTCGATAAt     <  2:1448506/140‑1 (MQ=255)

                                                                                                                                  tcaACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCAATACCATAAACGTTCGGGAAGCGAATTTCCGGTGCCGCAGAAGCGAGGTACACTTTCTTCGCTCCGGCTTCGCGTGCCATCTCGATAATCTg  >  1:783760/1‑140 (MQ=255)

                                                                                                                                  tcaACTCCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCAATACCATAAACGTTCGGGAAGCGAATTTCCGGTGCCGCAGAAGCGAGGTACACTTTCTTCGCTCCGGCTTCGCGTGCCATCTCGATAATCTg  >  2:1617681/1‑140 (MQ=255)

GACGTAGACGCCGTTGAACACCGAGCATTCAAACTGCTGGATATCCGGATTTTCAGCGCGAACGGCGTCGATCAGATCGTTCAGATCCTGGAAAATCAACCCGTCAGCACCGATGATCTGGCGAATTTCATCAACTTCGCGACCGTGAGCGATCAGTTCCGTGGCGCTCGGCATATCAATACCATAAACGTTCGGGAAGCGAATTTCCGGTGCCGCAGAAGCGAGGTACACTTTCTTCGCTCCGGCTTCGCGTGCCATCTCGATAATCTG  >  NC_000913/2428838‑2429107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A