Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F60 I1 R1	20	57.0	1936532	99.1%	1919103	138.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,489,144	G→A	P9S (CCG→TCG)	yfdE ←	acetyl‑CoA:oxalate CoA‑transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,489,144	0	G	A	100.0%	202.7 / NA	59	P9S (CCG→TCG)	yfdE	acetyl‑CoA:oxalate CoA‑transferase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (33/26); total (33/26)

ACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGG  >  NC_000913/2489007‑2489277

aCCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGAcc                                                                                                                                     >  1:512349/1‑140 (MQ=255)

          cGGGTATCATCACCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGACCCTTTGCTTTc                                                                                                                           <  1:185403/140‑1 (MQ=255)

               atcatcACCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGACCCTTTGCTTTCattat                                                                                                                      <  1:635659/140‑1 (MQ=255)

                     aCCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGACCCTTTGCTTTCATTATTTGTCa                                                                                                                <  1:554405/140‑1 (MQ=255)

                         tGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGACCCTTTGCTTTCATTATTTGTCa                                                                                                                >  2:300375/1‑136 (MQ=255)

                         tGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGACCCTTTGCTTTCATTATTTGTCa                                                                                                                <  1:300375/136‑1 (MQ=255)

                         tGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGACCCTTTGCTTTCATTATTTGTCATTTg                                                                                                            >  1:290708/1‑140 (MQ=255)

                           aCCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGACCCTTTGCTTTCATTATTTGTCATTTGCt                                                                                                          <  1:850159/140‑1 (MQ=255)

                               ggcggcTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGACCCTTTGCTTTCATTATTTGTCATTTGCTTATg                                                                                                      >  2:858693/1‑140 (MQ=255)

                                 cggcTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGACCCTTTGCTTTCATTATTTGTCATTTGCTTATGcc                                                                                                    >  1:346024/1‑140 (MQ=255)

                                 cggcTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGACCCTTTGCTTTCATTATTTGTCATTTGCTTATGcc                                                                                                    >  1:399342/1‑140 (MQ=255)

                                 cggcTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGACCCTTTGCTTTCATTATTTGTCATTTGCTTATGcc                                                                                                    <  2:399342/140‑1 (MQ=255)

                                    cTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGACCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTg                                                                                                 >  2:434932/1‑140 (MQ=255)

                                              aTTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGACCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCg                                                                                       <  2:290708/140‑1 (MQ=255)

                                                tACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGACCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGaa                                                                                     <  2:558997/140‑1 (MQ=255)

                                                tACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGACCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGaa                                                                                     <  1:411174/140‑1 (MQ=255)

                                                        cgcCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGACCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGaaaa                                                                             <  2:512349/140‑1 (MQ=255)

                                                                 tACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGACCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCg                                                                    >  2:467569/1‑140 (MQ=255)

                                                                         agTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGACCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTAt                                                            >  1:18536/1‑140 (MQ=255)

                                                                          gTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGACCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCAt                                                                <  1:805816/135‑1 (MQ=255)

                                                                          gTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGACCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCAt                                                                >  2:805816/1‑135 (MQ=255)

                                                                           ttGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGACCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGt                                                          >  1:693129/1‑140 (MQ=255)

                                                                             gAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGACCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTaa                                                        <  2:693129/140‑1 (MQ=255)

                                                                                   cGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGACCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCaa                                                  <  1:434932/140‑1 (MQ=255)

                                                                                    gAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGACCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAAt                                                 >  1:482710/1‑140 (MQ=255)

                                                                                    gAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGACCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAAt                                                 <  1:510907/140‑1 (MQ=255)

                                                                                     aaaGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGACCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATa                                                >  1:260885/1‑140 (MQ=255)

                                                                                        ggTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGACCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACa                                             <  1:467569/140‑1 (MQ=255)

                                                                                              ttAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGACCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGatta                                       <  1:858693/140‑1 (MQ=255)

                                                                                                 aGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGACCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGattaatt                                    <  2:18536/140‑1 (MQ=255)

                                                                                                 aGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGACCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGattaatt                                    >  2:635405/1‑140 (MQ=255)

                                                                                                      aTGTGTCATATCGATAACTAATAAGCCTTCAAACGACCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGaa                               >  1:459128/1‑140 (MQ=255)

                                                                                                        gtgtCATATCGATAACTAATAAGCCTTCAAACGACCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGa                                >  1:645937/1‑137 (MQ=255)

                                                                                                        gtgtCATATCGATAACTAATAAGCCTTCAAACGACCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGa                                <  2:645937/137‑1 (MQ=255)

                                                                                                        gtgtCATATCGATAACTAATAAGCCTTCAAACGACCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAAc                             >  1:946160/1‑140 (MQ=255)

                                                                                                        gtgtCATATCGATAACTAATAAGCCTTCAAACGACCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAAc                             >  1:425198/1‑140 (MQ=255)

                                                                                                           tCATATCGATAACTAATAAGCCTTCAAACGACCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAg                          <  1:635405/140‑1 (MQ=255)

                                                                                                                tCGATAACTAATAAGCCTTCAAACGACCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGAc                     <  2:482710/140‑1 (MQ=255)

                                                                                                                   aTAACTAATAAGCCTTCAAACGACCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATTATTAATTATGAAACCAGTCGACTGa                  >  1:462791/1‑140 (MQ=255)

                                                                                                                   aTAACTAATAAGCCTTCAAACGACCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGa                  >  2:119422/1‑140 (MQ=255)

                                                                                                                   aTAACTAATAAGCCTTCAAACGACCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGa                  >  2:712831/1‑140 (MQ=255)

                                                                                                                   aTAACTAATAAGCCTTCAAACGACCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGa                  >  2:748850/1‑140 (MQ=255)

                                                                                                                   aTAACTAATAAGCCTTCAAACGACCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGa                  >  2:440741/1‑140 (MQ=255)

                                                                                                                   aTAACTAATAAGCCTTCAAACGACCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGa                  >  2:516432/1‑140 (MQ=255)

                                                                                                                      aCTAATAAGCCTTCAAACGACCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACAt               <  2:459128/140‑1 (MQ=255)

                                                                                                                      aCTAATAAGCCTTCAAACGACCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACAt               <  1:440741/140‑1 (MQ=255)

                                                                                                                      aCTAATAAGCCTTCAAACGACCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACAt               <  1:119422/140‑1 (MQ=255)

                                                                                                                             aGCCTTCAAACGACCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCca        >  1:178712/1‑140 (MQ=255)

                                                                                                                              gCCTTCAAACGACCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCcac       >  1:724920/1‑140 (MQ=255)

                                                                                                                                cTTCAAACGACCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACaa     <  2:724920/140‑1 (MQ=255)

                                                                                                                                 ttCAAACGACCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAg    <  2:425198/140‑1 (MQ=255)

                                                                                                                                  tCAAACGACCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACAt               <  2:55024/128‑1 (MQ=255)

                                                                                                                                  tCAAACGACCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACAt               >  1:55024/1‑128 (MQ=255)

                                                                                                                                  tCAAACGACCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAgg   >  2:558890/1‑140 (MQ=255)

                                                                                                                                  tCAAACGACCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAgg   >  2:656163/1‑140 (MQ=255)

                                                                                                                                  tCAAACGACCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAgg   >  2:760447/1‑140 (MQ=255)

                                                                                                                                  tCAAACGACCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAgg   >  2:795330/1‑140 (MQ=255)

                                                                                                                                  tCAAACGACCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAgg   >  2:95721/1‑140 (MQ=255)

                                                                                                                                   cAAACGACCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAggg  >  1:448176/1‑140 (MQ=255)

ACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGG  >  NC_000913/2489007‑2489277

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A