Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A42 F60 I1 R1	19	81.6	2766894	99.0%	2739225	138.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,540,397	C→T	G140S (GGC→AGC)	cysA ←	sulfate/thiosulfate ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,540,397	0	C	T	100.0%	113.1 / NA	34	G140S (GGC→AGC)	cysA	sulfate/thiosulfate ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (16/18); total (16/18)

CTTCATGGAGTTGACGCAGCCAGCGACGCAGCTCTTTACGCACCTGCGCATCCAGCGCGCCAAACGGTTCATCAAGCAGCAGAATTTGCGGTTCCACAGCCAGCGCGCGCGCCAGCGCCACGCGCTGTTTCTGGCCGCCGGAAAGCTGCGCCGGATAACGATCCGCCAGATGGGCAAGCTGGACCATTTCCAGCAATTTTGTCACTTTCGCTTTGATGGCTGCGGCATTCGGGCGCTCGCGACGCGGCAGCACCGTCAGGCCAAAAGCGATA  >  NC_000913/2540262‑2540533

cttcATGGAGTTGACGCAGCCAGCGACGCAGCTCTTTACGCACCTGCGCATCCAGCGCGCCAAACGGTTCATCAAGCAGCAGAATTTGCGGTTCCACAGCCAGCGCGCGCGCCAGCGCCACGCGCTGTTTCTGGctgccg                                                                                                                                      <  2:1314314/140‑1 (MQ=255)

cttcATGGAGTTGACGCAGCCAGCGACGCAGCTCTTTACGCACCTGCGCATCCAGCGCGCCAAACGGTTCATCAAGCAGCAGAATTTGCGGTTCCACAGCCAGCGCGCGCGCCAGCGCCACGCGCTGTTTCTGGctgccg                                                                                                                                      <  2:773816/140‑1 (MQ=255)

        aGTTGACGCAGCCAGCGACGCAGCTCTTTACGCACCTGCGCATCCAGCGCGCCAAACGGTTCATCAAGCAGCAGAATTTGCGGTTCCACAGCCAGCGCGCGCGCCAGCGCCACGCGCTGTTTCTGGCTGCCGGAAAGCTg                                                                                                                              <  2:350034/140‑1 (MQ=255)

          ttGACGCAGCCAGCGACGCAGCTCTTTACGCACCTGCGCATCCAGCGCGCCAAACGGTTCATCAAGCAGCAGAATTTGCGGTTCCACAGCCAGCGCGCGCGCCAGCGCCACGCGCTGTTTCTGGCTGCCGGAAAGCTgcg                                                                                                                            <  2:497286/140‑1 (MQ=255)

          ttGACGCAGCCAGCGACGCAGCTCTTTACGCACCTGCGCATCCAGCGCGCCAAACGGTTCATCAAGCAGCAGAATTTGCGGTTCCACAGCCAGCGCGCGCGCCAGCGCCACGCGCTGTTTCTGGCTGCCGGAAAGCTgcg                                                                                                                            <  2:466886/140‑1 (MQ=255)

          ttGACGCAGCCAGCGACGCAGCTCTTTACGCACCTGCGCATCCAGCGCGCCAAACGGTTCATCAAGCAGCAGAATTTGCGGTTCCACAGCCAGCGCGCGCGCCAGCGCCACGCGCTGTTTCTGGCTGCCGGAAAGCTgcg                                                                                                                            >  1:497286/1‑140 (MQ=255)

           tGACGCAGCCAGCGACGCAGCTCTTTACGCACCTGCGCATCCAGCGCGCCAAACGGTTCATCAAGCAGCAGAATTTGCGGTTCCACAGCCAGCGCGCGCGCCAGCGCCACGCGCTGTTTCTGGCTGCCGGAAAGCTgcgc                                                                                                                           >  2:80037/1‑140 (MQ=255)

           tGACGCAGCCAGCGACGCAGCTCTTTACGCACCTGCGCATCCAGCGCGCCAAACGGTTCATCAAGCAGCAGAATTTGCGGTTCCACAGCCAGCGCGCGCGCCAGCGCCACGCGCTGTTTCTGGCTGCCGGAAAGCTgcgc                                                                                                                           >  2:462071/1‑140 (MQ=255)

            gACGCAGCCAGCGACGCAGCTCTTTACGCACCTGCGCATCCAGCGCGCCAAACGGTTCATCAAGCAGCAGAATTTGCGGTTCCACAGCCAGCGCGCGCGCCAGCGCCACGCGCTGTTTCTGGCTGCCGGAAAGCTGCGcc                                                                                                                          >  1:250960/1‑140 (MQ=255)

            gACGCAGCCAGCGACGCAGCTCTTTACGCACCTGCGCATCCAGCGCGCCAAACGGTTCATCAAGCAGCAGAATTTGCGGTTCCACAGCCAGCGCGCGCGCCAGCGCCACGCGCTGTTTCTGGCTGCCGGAAAGCTGCGcc                                                                                                                          <  1:695607/140‑1 (MQ=255)

            gACGCAGCCAGCGACGCAGCTCTTTACGCACCTGCGCATCCAGCGCGCCAAACGGTTCATCAAGCAGCAGAATTTGCGGTTCCACAGCCAGCGCGCGCGCCAGCGCCACGCGCTGTTTCTGGCTGCCGGAAAGCTGCGcc                                                                                                                          >  2:1114668/1‑140 (MQ=255)

            gACGCAGCCAGCGACGCAGCTCTTTACGCACCTGCGCATCCAGCGCGCCAAACGGTTCATCAAGCAGCAGAATTTGCGGTTCCACAGCCAGCGCGCGCGCCAGCGCCACGCGCTGTTTCTGGCTGCCGGAAAGCTGCGcc                                                                                                                          >  2:1106888/1‑140 (MQ=255)

                  gccagcGACGCAGCTCTTTACGCACCTGCGCATCCAGCGCGCCAAACGGTTCATCAAGCAGCAGAATTTGCGGTTCCACAGCCAGCGCGCGCGCCAGCGCCACGCGCTGTTTCTGGCTGCCGGAAAGCTGCGCCGGATaa                                                                                                                    >  1:604192/1‑140 (MQ=255)

                  gccagcGACGCAGCTCTTTACGCACCTGCGCATCCAGCGCGCCAAACGGTTCATCAAGCAGCAGAATTTGCGGTTCCACAGCCAGCGCGCGCGCCAGCGCCACGCGCTGTTTCTGGCTGCCGGAAAGCTGCGCCGGATaa                                                                                                                    >  1:1246844/1‑140 (MQ=255)

                    cagcGACGCAGCTCTTTACGCACCTGCGCATCCAGCGCGCCAAACGGTTCATCAAGCAGCAGAATTTGCGGTTCCACAGCCAGCGCGCGCGCCAGCGCCACGCGCTGTTTCTGGCTGCCGGAAAGCTGCGCCGGATAACg                                                                                                                  <  2:4024/140‑1 (MQ=255)

                             aGCTCTTTACGCACCTGCGCATCCAGCGCGCCAAACGGTTCATCAAGCAGCAGAATTTGCGGTTCCACAGCCAGCGCGCGCGCCAGCGCCACGCGCTGTTTCTGGCTGCCGGAAAGCTGCGCCGGATAACGATCCGCCAg                                                                                                         <  2:1209169/140‑1 (MQ=255)

                             aGCTCTTTACGCACCTGCGAATCCAGCGCGCCAAACGGATCATCAAGCACCAGAATTTGCGGTTCCACAGCCAGCGCGCGCGCCAGCGCCACGCGCTGTTTCTGGCTGCCGGAAAGCTGCGCCGGATAACGAACCGCCAg                                                                                                         >  1:516178/1‑140 (MQ=255)

                                       gCACCTGCGCATCCAGCGCGCCAAACGGTTCATCAAGCAGCAGAATTTGCGGTTCCACAGCCAGCGCGCGCGCCAGCGCCACGCGCTGTTTCTGGCTGCCGGAAAGCTGCGCCGGATAACGATTCGCCAGATGGGCAAGc                                                                                               <  2:1369336/140‑1 (MQ=255)

                                         aCCTGCGCATCCAGCGCGCCAAACGGTTCATCAAGCAGCAGAATTTGCGGTTCCACAGCCAGCGCGCGCGCCAGCGCCACGCGCTGTTTCTGGCTGCCGGAAAGCTGCGCCGGATAACGATCCGCCAGATGGGc                                                                                                   <  2:98796/134‑1 (MQ=255)

                                         aCCTGCGCATCCAGCGCGCCAAACGGTTCATCAAGCAGCAGAATTTGCGGTTCCACAGCCAGCGCGCGCGCCAGCGCCACGCGCTGTTTCTGGCTGCCGGAAAGCTGCGCCGGATAACGATCCGCCAGATGGGc                                                                                                   >  1:98796/1‑134 (MQ=255)

                                         aCCTGCGCATCCAGCGCGCCAAACGGTTCATCAAGCAGCAGAATTTGCGGTTCCACAGCCAGCGCGCGCGCCAGCGCCACGCGCTGTTTCTGGCTGCCGGAAAGCTGCGCCGGATAACGATCCGCCAGATGGGCAAGCTg                                                                                             >  2:1211391/1‑140 (MQ=255)

                                           cTGCGCATCCAGCGCGCAAAACGGTTCATCAAGCAGCAGAATTTGCGGTTCCACAGCCAGCGCGCGCGCCAGCGCCACGCGCTGTTTCTGGCTGCCGGAAAGCTGCGCCGGATAACGATCCGCCAGATGGGCAAGCTGGa                                                                                           <  1:462071/140‑1 (MQ=255)

                                            tGCGCATCCAGCGCGCCAAACGGTTCATCAAGCAGCAGAATTTGCGGTTCCACAGCCAGCGCGCGCGCCAGCGCCACGCGCTGTTTCTGGCTGCCGGAAAGCTGCGCCGGATAACGATCCGCCAGATGGGCAAGCTGGAc                                                                                          >  1:271144/1‑140 (MQ=255)

                                              cgcATCCAGCGCGCCAAACGGTTCATCAAGCAGCAGAATTTGCGGTTCCACAGCCAGCGCGCGCGCCAGCGCCACGCGCTGTTTCTGGCTGCCGGAAAGCTGCGCCGGATAACGATCCGCCAGATGGGCAAGCTGGACCa                                                                                        <  1:1106888/140‑1 (MQ=255)

                                                    cAGCGCGCCAAACGGTTCATCAAGCAGCAGAATTTGCGGTTCCACAGCCAGCGCGCGCGCCAGCGCCACGCGCTGTTTCTGGCTGCCGGAAAGCTGCGCCGGATAACGATCCGCCAGATGGGCAAGCTGGACCATTTCCa                                                                                  <  2:1246844/140‑1 (MQ=255)

                                                         cgcCAAACGGTTCATCAAGCAGCAGAATTTGCGGTTCCACAGCCAGCGCGCGCGCCAGCGCCACGCGCTGTTTCTGGCTGCCGGAAAGCTGCGCCGGATAACGATCCGCCAGATGGGCAAGCTGGACCATTTCCAGCAAt                                                                             <  2:271144/140‑1 (MQ=255)

                                                              aaCGGTTCATCAAGCAGCAGAATTTGCGGTTCCACAGCCAGCGCGCGCGCCAGCGCCACGCGCTGTTTCTGGCTGCCGGAAAGCTGCGCCGGATAACGATCCGCCAGATGGGCAAGCTGGACCATTTCCAGCAATTTTGt                                                                        <  1:716165/140‑1 (MQ=255)

                                                              aaCGGTTCATCAAGCAGCAGAATTTGCGGTTCCACAGCCAGCGCGCGCGCCAGCGCCACGCGCTGTTTCTGGCTGCCGGAAAGCTGCGCCGGATAACGATCCGCCAGATGGGCAAGCTGGACCATTTCCAGCAATTTTGt                                                                        <  1:1161629/140‑1 (MQ=255)

                                                                    tcataaAGCAGCAGAATTTGCGGTTCCACAGCCAGCGCGCGCGCGAGCGCCACGCGGTGTTTCTGGCTGCCGGAAAGCTGCGCCGGATAACGATCCGCCAGCTGGGCAAGCTGGCCCATTTCCAGCAATTTTGTCACttt                                                                  >  2:960659/1‑140 (MQ=255)

                                                                     catcaAGCAGCAGAATTTGCGGTTCCACAGCCAGCGCGCGCGCCAGCGCCACGCGCTGTTTCTGGCTGCCGGAAAGCTGCGCCGGATAACGATCCGCCAGATGGGCAAGCTGGACCATTTCCAGCAATTTTGTCACTTTc                                                                 <  2:604192/140‑1 (MQ=255)

                                                                                agAATTTGCGGTTCCACAGCCAGCGCGCGCGCCAGCGCCACGCGCTGTTTCTGGCTGCCGGAAAGCTGCGCCGGATAACGATCCGCCAGATGGGCAAGCTGGACCATTTCCAGCAATTTTGTCACTTTCGCTTTGATGGc                                                      >  1:649706/1‑140 (MQ=255)

                                                                                    tttGCGGTTCCACAGCCAGCGCGCGCGCCAGCGCCACGCGCTGTTTCTGGCTGCCGGAAAGCTGCGCCGGATAACGATCCGCCAGATGGGCAAGCTGGACCATTTCCAGCAATTTTGTCACTTTCGCTTTGATGGCTGCg                                                  >  2:82571/1‑140 (MQ=255)

                                                                                     ttGCGGTTCCACAGCCAGCGCGCGCGCCAGCGCCACGCGCTGTTTCTGGCTGCCGGAAAGCTGCGCCGGATAACGATCCGCCAGATGGGCAAGCTGGACCATTTCCAGCAATTTTGTCACTTTCGCTTTGATGGCTGCgg                                                 <  1:1211391/140‑1 (MQ=255)

                                                                                                     agcGCGCGCGCCAGCGCCACGCGCTGTTTCTGGCTGCCGGAAAGCTGCGCCGGATAACGATCCGCCAGATGGGCAAGCTGGACCATTTCCAGCAATTTTGTCACTTTCGCTTTGATGGCTGCGGCATTCGGGCGCTcgcg                                 >  1:192400/1‑140 (MQ=255)

                                                                                                     agcGCGCGCGCCAGCGCCACGCGCTGTTTCTGGCTGCCGGAAAGCTGCGCCGGATAACGATCCGCCAGATGGGCAAGCTGGACCATTTCCAGCAATTTTGTCACTTTCGCTTTGATGGCTGCGGCATTCGGGCGCTcgcg                                 >  2:61813/1‑140 (MQ=255)

                                                                                                       cgcgcgcgcCAGCGCCACGCGCTGTTTCTGGCTGCCGGAAAGCTGCGCCGGATAACGATCCGCCAGATGGGCAAGCTGGACCATTTCCAGCAATTTTGTCACTTTCGCTTTGATGGCTGCGGCATTCGGGCGCTCGCGAc                               >  1:1068166/1‑140 (MQ=255)

                                                                                                                    gccaCGCGCTGTTTCTGGCTGCCGGAAAGCTGCGCCGGATAACGATCCGCCAGATGGGCAAGCTGGACCATTTCCAGCAATTTTGTCACTTTCGCTTTGATGGCTGCGGCATTCGGGCGCTCGCGACGCGGCAGCACCGt                  <  1:80037/140‑1 (MQ=255)

                                                                                                                                    ggCTGCCGGAAAGCTGCGCCGGATAACGATCCGCCAGATGGGCAAGCTGGACCATTTCCAGCAATTTTGTCACTTTCGCTTTGATGGCTGCGGCATTCGGGCGCTCGCGACGCGGCAGCACCGTCAGGCCAAAAGCGata  <  1:61813/140‑1 (MQ=255)

CTTCATGGAGTTGACGCAGCCAGCGACGCAGCTCTTTACGCACCTGCGCATCCAGCGCGCCAAACGGTTCATCAAGCAGCAGAATTTGCGGTTCCACAGCCAGCGCGCGCGCCAGCGCCACGCGCTGTTTCTGGCCGCCGGAAAGCTGCGCCGGATAACGATCCGCCAGATGGGCAAGCTGGACCATTTCCAGCAATTTTGTCACTTTCGCTTTGATGGCTGCGGCATTCGGGCGCTCGCGACGCGGCAGCACCGTCAGGCCAAAAGCGATA  >  NC_000913/2540262‑2540533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A