Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A46 F60 I1 R1 17 83.7 2854354 99.1% 2828664 138.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,542,485 G→A L10L (CTG→TTG)  cysU ← sulfate/thiosulfate ABC transporter inner membrane subunit CysU

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,542,4850GA100.0% 155.0 / NA 46L10L (CTG→TTG) cysUsulfate/thiosulfate ABC transporter inner membrane subunit CysU
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/16);  total (30/16)

CTGCGGGTTGGTGATCACCTCCCAGTACTGCGCCCAGCTCATCTGGGCCAGTTGCATCACCAGCGCGGAGAGCGGCAGCAGCAAAATCAGGCACACAAACAGCAGACTGGTGCCGAGGCTTAAGG‑TAAAGCCCGGCAGCACGCGTCTGGAGGAGACAGCAAACATCAGTTACGCCCCGCCGCTAACAGCTTGTCTAACTCGCCGCCGCTGGTGAAGTGGGTTTTCATCACTTCCGGCCAGGAGCCAAATTTGTCTTCCACGCGGAACA  >  NC_000913/2542348‑2542615
                                                                                                                                          |                                                                                                                                  
cTGCGGGTTGGTGATCACCTCCCAGTACTGCGCCCAGCTCATCTGGGCCAGTTGCATCACCAGCGCGGAGAGCGGCAGCAGCAAAATCAGGCACACAAACAGCAGACTGGTGCCGAGGCTTAAGG‑TAAAGCCCGgcaaca                                                                                                                                  <  2:703079/140‑1 (MQ=255)
cTGCGGGTTGGTGATCACCTCCCAGTACTGCGCCCAGCTCATCTGGGCCAGTTGCATCACCAGCGCGGAGAGCGGCAGCAGCAAAATCAGGCACACAAACAGCAGACTGGTGCCGAGGCTTAAGG‑TAAAGCCCGgcaaca                                                                                                                                  <  2:1196039/140‑1 (MQ=255)
 tGCGGGTTGGTGATCACCTCCCAGTACTGCGCCCAGCTCATCTGGGCCAGTTGCATCACCAGCGCGGAGAGCGGCAGCAGCAAAATCAGGCACACAAACAGCAGACTGGTGCCGAGGCTTAAGG‑TAAAGCCCGGCAacac                                                                                                                                 <  1:924936/140‑1 (MQ=255)
     ggTTGGTGATCACCTCCCAGTACTGCGCCCAGCTCATCTGGGCCAGTTGCATCACCAGCGCGGAGAGCGGCAGCAGCAAAATCAGGCACACAAACAGCAGACTGGTGCCGAGGCTTAAGG‑TAAAGCCCGGCAACACGCGt                                                                                                                             <  2:683244/140‑1 (MQ=255)
               cACCTCCCAGTACTGCGCCCAGCTCATCTGGGCCAGTTGCATCACCAGCGCGGAGAGCGGCAGCAGCAAAATCAGGCACACAAACAGCAGACTGGTGCCGAGGCTTAAGG‑TAAAGCCCGGCAACACGCGTCTGGAGgaga                                                                                                                   <  1:772036/140‑1 (MQ=255)
               cACCTCCCAGTACTGCGCCCAGCTCATCTGGGCCAGTTGCATCACCAGCGCGGAGAGCGGCAGCAGCAAAATCAGGCACACAAACAGCAGACTGGTGCCGAGGCTTAAGG‑TAAAGCCCGGCAACACGCGTCTGGAGgaga                                                                                                                   >  1:683091/1‑140 (MQ=255)
                   tCCCAGTACTGCGCCCAGCTCATCTGGGCCAGTTGCATCACCAGCGCGGAGAGCGGCAGCAGCAAAATCAGGCACACAAACAGCAGACTGGTGCCGAGGCTTAAGG‑TAAAGCCCGGCAACACGCGTCTGGAGGAGACAGc                                                                                                               <  2:584018/140‑1 (MQ=255)
                         tACTGCGCCCAGCTCATCTGGGCCAGTTGCATCACCAGCGCGGAGAGCGGCAGCAGCAAAATCAGGCACACAAACAGCAGACTGGTGCCGAGGCTTAAGG‑TAAAGCCCGGCAACACGCGTCTGGAGGAGACa                                                                                                                 >  2:304997/1‑132 (MQ=255)
                         tACTGCGCCCAGCTCATCTGGGCCAGTTGCATCACCAGCGCGGAGAGCGGCAGCAGCAAAATCAGGCACACAAACAGCAGACTGGTGCCGAGGCTTAAGG‑TAAAGCCCGGCAACACGCGTCTGGAGGAGACa                                                                                                                 <  1:304997/132‑1 (MQ=255)
                              cgcCCAGCTCATCTGGGCCAGTTGCATCACCAGCGCGGAGAGCGGCAGCAGCAAAATCAGGCACACAAACAGCAGACTGGTGCCGAGGCTTAAGG‑TAAAGCCCGGCAACACGCGTCTGGAGGAGACAGCAAACAt                                                                                                         >  2:549014/1‑135 (MQ=255)
                              cgcCCAGCTCATCTGGGCCAGTTGCATCACCAGCGCGGAGAGCGGCAGCAGCAAAATCAGGCACACAAACAGCAGACTGGTGCCGAGGCTTAAGG‑TAAAGCCCGGCAACACGCGTCTGGAGGAGACAGCAAACAt                                                                                                         <  1:549014/135‑1 (MQ=255)
                              cgcCCAGCTCATCTGGGCCAGTTGCATCACCAGCGCGGAGAGCGGCAGCAGCAAAATCAGGCACACAAACAGCAGACTGGTGCCGAGGCTTAAGG‑TAAAGCCCGGCAACACGCGTCTGGAGGAGACAGCAAACATCAGtt                                                                                                    >  1:518247/1‑140 (MQ=255)
                              cgcCCAGCTCATCTGGGCCAGTTGCATCACCAGCGCGCAGAGCGGCAGCAGCAAAATCAGGCACACAAACAGCAGACTGGTGCCGAGGCTTAAGG‑TAAAGCCCGGCAACACGCGTCTGGAGGAGACAGCAAACATCAGtt                                                                                                    >  1:218815/1‑140 (MQ=255)
                               gcCCAGCTCATCTGGGCCAGTTGCATCACCAGCGCGGAGAGCGGCAGCAGCAAAATCAGGCACACAAACAGCAGACTGGTGCCGAGGCTTAAGG‑TAAAGCCCGGCAACACGCGTCTGGAGGAGACAGCAAACATCAGTTa                                                                                                   >  2:992874/1‑140 (MQ=255)
                                   aGCTCATCTGGGCCAGTTGCATCACCAGCGCGGAGAGCGGCAGCAGCAAAATCAGGCACACAAACAGCAGACTGGTGCCGAGGCTTAAGG‑TAAAGCCCGGCAACACGCGTCTGGAGGAGACAGCa                                                                                                              >  2:894770/1‑125 (MQ=255)
                                   aGCTCATCTGGGCCAGTTGCATCACCAGCGCGGAGAGCGGCAGCAGCAAAATCAGGCACACAAACAGCAGACTGGTGCCGAGGCTTAAGG‑TAAAGCCCGGCAACACGCGTCTGGAGGAGACAGCa                                                                                                              <  1:894770/125‑1 (MQ=255)
                                   aGCTCATCTGGGCCAGTTGCATCACCAGCGCGGAGAGCGGCAGCAGCAAAATCAGGCACACAAACAGCAGACTGGTGCCGAGGCTTAAGG‑TAAAGCCCGGCAACACGCGTCTGGAGGAGACAGCAAACATCAGTTACGcc                                                                                               >  2:1381447/1‑140 (MQ=255)
                                        aTCTGGGCCAGTTGCATCACCAGCGCGGAGAGCGGCAGCAGCAAAATCAGGCACACAAACAGCAGACTGGTGCCGAGGCTTAAGG‑TAAAGCCCGGCAACACGCGTCTGGAGGAGACAGCAAACATCAGTTACGCCccgcc                                                                                          >  2:820209/1‑140 (MQ=255)
                                        aTCTGGGCCAGTTGCATCACCAGCGCGGAGAGCGGCAGCAGCAAAATCAGGCACACAAACAGCAGACTGGTGCCGAGGCTTAAGG‑TAAAGCCCGGCAACACGCGTCTGGAGGAGACAGCAAACATCAGTTACGCCccgcc                                                                                          >  1:1092104/1‑140 (MQ=255)
                                           tGGGCCAGTTGCATCACCAGCGCGGAGAGCGGCAGCAGCAAAATCAGGCACACAAACAGCAGACTGGTGCCGAGGCTTAAGG‑TAAAGCCCGGCAACACGCGTCTGGAGGAGACAGCAAACATCAGTTACGCCCCGCCGCt                                                                                       <  1:1376335/140‑1 (MQ=255)
                                             ggCCAGTTGCATCACCAGCGCGGAGAGCGGCAGCAGCAAAATCAGGCACACAAACAGCAGACTGGTGCCGAGGCTTAAGG‑TAAAGCCCGGCAACACGCGTCTGGAGGAGACAGCAAACATCAGTTACGCCCCGCCGCTaa                                                                                     <  2:683091/140‑1 (MQ=255)
                                                   ttGCATCACCAGCGCGGAGAGCGGCAGCAGCAAAATCAGGCACACAAACAGCAGACTGGTGCCGAGGCTTAAGG‑TAAAGCCCGGCAACACGCGTCTGGAGGAGACAGCAAACATCAGTTACGCCCCGCCGCTAACAGCtt                                                                               >  1:1029916/1‑140 (MQ=255)
                                                        tCACCAGCGCGGAGAGCGGCAGCAGCAAAATCAGGCACACAAACAGCAGACTGGTGCCGAGGCTTAAGG‑TAAAGCCCGGCAACACGCGTCTGGAGGAGACAGCAAACATCAGTTACGCCCCGCCGCTAACAGCTTGTCTa                                                                          >  1:241291/1‑140 (MQ=255)
                                                            cAGCGCGGAGAGCGGCAGCAGCAAAATCAGGCACACAAACAGCAGACTGGTGCCGAGGCTTAAGG‑TAAAGCCCGGCAACACGCGTCTGGAGGAGACAGCAAACATCAGTTACGCCCCGCCGCTAACAGCTTGTCTAACTc                                                                      >  1:172424/1‑140 (MQ=255)
                                                               cgcgGAGAGCGGCAGCAGCAAAATCAGGCACACAAACAGCAGACTGGTGCCGAGGCTTAAGG‑TAAAGCCCGGCAACACGCGTCTGGAGGAGACAGCAAACATCAGTTACGCCCCGCCGCTAACAGCTTGTCTAACTcgcc                                                                   <  1:1312981/140‑1 (MQ=255)
                                                               cgcgGAGAGCGGCAGCAGCAAAATCAGGCACACAAACAGCAGACTGGTGCCGAGGCTTAAGG‑TAAAGCCCGGCAACACGCGTCTGGAGGAGACAGCAAACATCAGTTACGCCCCGCCGCTAACAGCTTGTCTAACTcgcc                                                                   >  1:386205/1‑140 (MQ=255)
                                                               cgcgGAGAGCGGCAGCAGCAAAATCAGGCACACAAACAGCAGACTGGTGCCGAGGCTTAAGG‑TAAAGCCCGGCAACACGCGTCTGGAGGAGACAGCAAACATCAGTTACGCCCCGCCGCTAACAGCTTGTCTAACTcgcc                                                                   >  2:1046231/1‑140 (MQ=255)
                                                                     gagCGGCAGCAGCAAAATCAGGCACACAAACAGCAGACTGGTGCCGAGGCTTAAGG‑TAAAGCCCGGCAACACGCGTCTGGAGGAGACAGCAAACATCAGTTACGCCCCGCCGCTAACAGCTTGTCTAACTCGCCGCCGCt                                                             <  1:1745/140‑1 (MQ=255)
                                                                      agCGGCAGCAGCAAAATCAGGCACACAAACAGCAGACTGGTGCCGAGGCTTAAGG‑TAAAGCCCGGCAACACGCGTCTGGAGGAGACAGCAAACATCAGTTACGCCCCGCCGCTAACAGCTTGTCTAACTCGCCGCCGCTg                                                            >  2:754285/1‑140 (MQ=255)
                                                                          gcagcagcaAAATCAGGCACACAAACAGCAGACTGGTGCCGAGGCTTAAGG‑TAAAGCCCGGCAACACGCGTCTGGAGGAGACAGCAAACATCAGTTACGCCCCGCCGCTAACAGCTTGTCTAACTCGCCGCCGCTGGTGa                                                        <  1:1381447/140‑1 (MQ=255)
                                                                            agcagcaAAATCAGGCACACAAACAGCAGACTGGTGCCGAGGCTTAAGG‑TAAAGCCCGGCAACACGCGTCTGGAGGAGACAGCAAACATCAGTTACGCCCCGCCGCTAACAGCTTGTCTAACTCGCCGCCGCTGGTGAAg                                                      <  1:992874/140‑1 (MQ=255)
                                                                                gcaAAATCAGGCACACAAACAGCAGACTGGTGCCGAGGCTTAAGG‑TAAAGCCCGGCAACACGCGTCTGGAGGAGACAGCAAACATCAGTTACGCCCCGCCGCTAACAGCTTGTCTAACTCGCCGCCGCTGGTGAAGTggg                                                  >  1:1366917/1‑140 (MQ=255)
                                                                                       cAGGCCCACAAACAGCAGATGGGTGCCGAGG‑TTAAGGTTAAAGCCGGGCAACCCGGTTCGGGAGGAGACGGCAAACAGCAGTTCCGCCCCGCCGCTAACAGCTTGTCTAACTCGCCGCCGCTGGTGAAGTGGGTTTtcat                                           <  2:1366917/140‑1 (MQ=255)
                                                                                                        gACGGGTGCCGAGGTTTAAGG‑TAAAGCCCGGAAACCCGCGTTTGGAGGAGACAGCAAACATCAGTTACGCCCCGCCGCTAACAGCTTGTCTAACTCGCCGCCGCTGGTGAAGTGGGTTTTCATCACTTCCGGCCAGGAGc                          <  2:958048/140‑1 (MQ=255)
                                                                                                         aCTGGTGCCGAGGCTTAAGG‑TAAAGCCCGGCAACACGCGTCTGGAGGAGACAGCAAACATCAGTTACGCCCCGCCGCTAACAGCTTGTCTAACTCGCCGCCGCTGGTGAAGTGGGTTTTCATCACTTCCGGCCAGGAGcc                         >  1:405915/1‑140 (MQ=255)
                                                                                                           tGGTGCCGAGGCTTAAGG‑TAAAGCCCGGCAACACGCGTCTGGAGGAGACAGCAAACATCAGTTACGCCCCGCCGCTAACAGCTTGTCTAACTCGCCGCCGCTGGTGAAGTGGGTTTTCATCACTTCCGGCCAGGAGCCaa                       >  1:231002/1‑140 (MQ=255)
                                                                                                              tGCCGAGGCTTAAGG‑TAAAGCCCGGCAACACGCGTCTGGAGGAGACAGCAAACATCAGTTACGCCCCGCCGCTAACAGCTTGTCTAACTCGCCGCCGCTGGTGAAGTGGGTTTTCATCACTTCCGGCCAGGAGCCAAAtt                    >  2:969263/1‑140 (MQ=255)
                                                                                                              tGCCGAGGCTTAAGG‑TAAAGCCCGGCAACACGCGTCTGGAGGAGACAGCAAACATCAGTTACGCCCCGCCGCTAACAGCTTGTCTAACTCGCCGCCGCTGGTGAAGTGGGTTTTCATCACTTCCGGCCAGGAGCCAAAtt                    >  1:433204/1‑140 (MQ=255)
                                                                                                              tGCCGAGGCTTAAGG‑TAAAGCCCGGCAACACGCGTCTGGAGGAGACAGCAAACATCAGTTACGCCCCGCCGCTAACAGCTTGTCTAACTCGCCGCCGCTGGTGAAGTGGGTTTTCATCACTTCCGGCCAGGAGCCAAAtt                    >  2:738812/1‑140 (MQ=255)
                                                                                                                   aGGCTTAAGG‑TAAAGCCCGGCAACACGCGTCTGGAGGAGACAGCAAACATCAGTTACGCCCCGCCGCTAACAGCTTGTCTAACTCGCCGCCGCTGGTGAAGTGGGTTTTCATCACTTCCGGcc                                >  2:289345/1‑123 (MQ=255)
                                                                                                                   aGGCTTAAGG‑TAAAGCCCGGCAACACGCGTCTGGAGGAGACAGCAAACATCAGTTACGCCCCGCCGCTAACAGCTTGTCTAACTCGCCGCCGCTGGTGAAGTGGGTTTTCATCACTTCCGGcc                                <  1:289345/123‑1 (MQ=255)
                                                                                                                    ggCTTAAGG‑TAAAGCCCGGCAACACGCGTCTGGAGGAGACAGCAAACATCAGTTACGCCCCGCCGCTAACAGCTTGTCTAACTCGCCGCCGCTGGTGAAGTGGGTTTTCATCACTTCCGGCCAGGAGCCAAATTTGTCtt              >  1:1018275/1‑140 (MQ=255)
                                                                                                                    ggCTTAAGG‑TAAAGCCCGGCAACACGCGTCTGGAGGAGACAGCAAACATCAGTTACGCCCCGCCGCTAACAGCTTGTCTAACTCGCCGCCGCTGGTGAAGTGGGTTTTCATCACTTCCGGCCAGGAGCCAAATTTGTCtt              <  1:754285/140‑1 (MQ=255)
                                                                                                                    ggCTTAAGG‑TAAAGCCCGGCAACACGCGTCTGGAGGAGACAGCAAACATCAGTTACGCCCCGCCGCTAACAGCTTGTCTAACTCGCCGCCGCTGGTGAAGTGGGTTTTCATCACTTCCGGCCAGGAGCCAAATTTGTCtt              <  2:172424/140‑1 (MQ=255)
                                                                                                                     gCTTAAGG‑TAAAGCCCGGCAACACGCGTCTGGAGGAGACAGCAAACATCAGTTACGCCCCGCCGCTAACAGCTTGTCTAACTCGCCGCCGCTGGTGAAGTGGGTTTTCATCACTTCCGGCCAGGAGCCAAATTTGTCTTc             >  1:1211047/1‑140 (MQ=255)
                                                                                                                        tAAGG‑TAAAGCCCGGCAACACGCGTCTGGAGGAGACAGCAAACATCAGTTACGCCCCGCCGCTAACAGCTTGTCTAACTCGCCGCCGCTGGTGAAGTGGGTTTTCATCACTTCCGGCCAGGAGCCAAATTTGTCTTCCAc          >  1:13830/1‑140 (MQ=255)
                                                                                                                         aaGG‑TAAAGCCCGGCAACACGCGTCTGGAGGAGACAGCAAACATCAGTTACGCCCCGCCGCTAACAGCTTGTCTAACTCGCCGCCGCTGGTGAAGTGGGTTTTCATCACTTCCGGCCAGGAGCCAAATTTGTCTTCCAcg         <  1:969263/140‑1 (MQ=255)
                                                                                                                          aGG‑TAAAGCCCGGCAACACGCGTCTGGAGGAGACAGCAAACATCAGTTACGCCCCGCCGCTAACAGCTTGTCTAACTCGCCGCCGCTGGTGAAGTGGGTTTTCATCACTTCCGGCCAGGAGCCAAATTTGTCTTCCAcgc        >  2:167435/1‑140 (MQ=255)
                                                                                                                                 aGCCCGGCAACACGCGTCTGGAGGAGACAGCAAACATCAGTTACGCCCCGCCGCTAACAGCTTGTCTAACTCGCCGCCGCTGGTGAAGTGGGTTTTCATCACTTCCGGCCAGGAGCCAAATTTGTCTTCCACGCGGAACa  >  1:1076853/1‑140 (MQ=255)
                                                                                                                                 aGCCCGGCAACACGCGTCTGGAGGAGACAGCAAACATCAGTTACGCCCCGCCGCTAACAGCTTGTCTAACTCGCCGCCGCTGGTGAAGTGGGTTTTCATCACTTCCGGCCAGGAGCCAAATTTGTCTTCCACGCGGAACa  >  1:1403981/1‑140 (MQ=255)
                                                                                                                                 aGCCCGGCAACACGCGTCTGGAGGAGACAGCAAACATCAGTTACGCCCCGCCGCTAACAGCTTGTCTAACTCGCCGCCGCTGGTGAAGTGGGTTTTCATCACTTCCGGCCAGGAGCCAAATTTGTCTTCCACGCGGAACa  >  1:789449/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                  
CTGCGGGTTGGTGATCACCTCCCAGTACTGCGCCCAGCTCATCTGGGCCAGTTGCATCACCAGCGCGGAGAGCGGCAGCAGCAAAATCAGGCACACAAACAGCAGACTGGTGCCGAGGCTTAAGG‑TAAAGCCCGGCAGCACGCGTCTGGAGGAGACAGCAAACATCAGTTACGCCCCGCCGCTAACAGCTTGTCTAACTCGCCGCCGCTGGTGAAGTGGGTTTTCATCACTTCCGGCCAGGAGCCAAATTTGTCTTCCACGCGGAACA  >  NC_000913/2542348‑2542615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A