Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F60 I1 R1	16	42.5	1464094	99.1%	1450917	138.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,552,222	A→G	intergenic (‑84/‑130)	ypeA ← / → amiA	putative acetyltransferase YpeA/N‑acetylmuramoyl‑L‑alanine amidase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,552,222	0	A	G	100.0%	151.4 / NA	41	intergenic (‑84/‑130)	ypeA/amiA	putative acetyltransferase YpeA/N‑acetylmuramoyl‑L‑alanine amidase A
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (15/26); total (15/26)

GTGATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATTCTCAACAGCAAACCGTCGTAACGGATTACGCGATACGATATAACATCTGGAACTTTATTATTACAACT  >  NC_000913/2552092‑2552343

gTGATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGGAAAAGTGGc                                                                                                                  <  1:618925/140‑1 (MQ=255)

gTGATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGGAAAAGTGGc                                                                                                                  <  1:530066/140‑1 (MQ=255)

   atgaCCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGGCGAAAATCGGGTGGAAAAGCGGCATa                                                                                                               >  2:540066/1‑140 (MQ=255)

   atgaCCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGGAAAAGTGGCATa                                                                                                               >  2:529912/1‑140 (MQ=255)

   atgaCCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGGAAAAGTGGCATa                                                                                                               >  2:149958/1‑140 (MQ=255)

        ctctTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGGAAAAGTGGCATAATggg                                                                                                          <  2:91837/140‑1 (MQ=255)

        ctctTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGGAAAAGTGGCATAATggg                                                                                                          <  1:540066/140‑1 (MQ=255)

          ctTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGGAAAAGTGGCATAATGGGGa                                                                                                        >  2:630736/1‑140 (MQ=255)

          ctTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGGAAAAGTGGCATAATGGGGa                                                                                                        >  2:422820/1‑140 (MQ=255)

           ttCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGGAAAAGTGGCATAATGGGGAg                                                                                                       >  2:249348/1‑140 (MQ=255)

                  tCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGGAAAAGTGGCATAATGGGGAGTTGTCaa                                                                                                <  1:396080/140‑1 (MQ=255)

                     tCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGGAAAAGTGGCATAATGGGGAGTTGTCAACTa                                                                                             >  2:326749/1‑140 (MQ=255)

                                          tcCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGGAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACaa                                                                        >  1:64676/1‑140 (MQ=255)

                                            cATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGGAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAAtt                                                                      <  1:251960/140‑1 (MQ=255)

                                            cATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGGAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAAtt                                                                      <  1:149958/140‑1 (MQ=255)

                                               ggTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGGAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATtctc                                                                   <  1:582750/140‑1 (MQ=255)

                                                   ttCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGGAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATTCTCAACa                                                               >  2:517062/1‑140 (MQ=255)

                                                      gTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGGAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATTCTCAACAGCa                                                            >  1:541929/1‑140 (MQ=255)

                                                        tACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGGAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATTCTCAACAGCaaa                                                          <  1:529912/140‑1 (MQ=255)

                                                           cTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGGAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATTCTCAACAGCAAACcg                                                       >  1:799/1‑140 (MQ=255)

                                                                tGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGGAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATTCTCAACAGCAAACCGTCGTa                                                  <  1:630736/140‑1 (MQ=255)

                                                                  cGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGGAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATTCTCAACAGCAAACCGTCGTAAc                                                >  1:637997/1‑140 (MQ=255)

                                                                       tAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGGAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATTCTCAACAGCAAACCGTCGTAACGGAtt                                           <  2:541929/140‑1 (MQ=255)

                                                                          aaGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGGAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATTCTCAACAGCAAACCGTCGTAACGGATTAcg                                        <  1:326749/140‑1 (MQ=255)

                                                                             gCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGGAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATTCTCAACAGCAAACCGTCGTAACGGATTACGcga                                     <  2:64676/140‑1 (MQ=255)

                                                                             gCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGGAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATTCTCAACAGCAAACCGTCGTAACGGATTACGcga                                     <  1:422820/140‑1 (MQ=255)

                                                                              cTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGGAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATTCTCAACAGCAAACCGTCGTAACGGATTACGcgat                                    <  2:637997/140‑1 (MQ=255)

                                                                               tGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGGAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATTCTCAACAGCAAACCGTCGTAACGGATTACGcgata                                   <  2:799/140‑1 (MQ=255)

                                                                               tGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGGAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATTCTCAACAGCAAACCGTCGTAACGGATTACGcgata                                   >  2:528881/1‑140 (MQ=255)

                                                                               tGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGGAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATTCTCAACAGCAAACCGTCGTAACGGATTACGcgata                                   <  1:517062/140‑1 (MQ=255)

                                                                               tGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGGAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATTCTCAACAGCAAACCGTCGTAACGGATTACGcgata                                   <  2:81051/140‑1 (MQ=255)

                                                                               tGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGGAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATTCTCAACAGCAAACCGTCGTAACGGATTACGcgata                                   <  1:175291/140‑1 (MQ=255)

                                                                                gATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGGAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATTCTCAACAGCAAACCGTCGTAACGGATTACGcgatac                                  <  2:206561/140‑1 (MQ=255)

                                                                                 aTTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGGAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATTCTCAACAGCAAACCGTCGTAACGGATTAcgcg                                      >  1:644758/1‑135 (MQ=255)

                                                                                 aTTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGGAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATTCTCAACAGCAAACCGTCGTAACGGATTAcgcg                                      <  2:644758/135‑1 (MQ=255)

                                                                                       gCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGGAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATTCTCAACAGCAAACCGTCGTAACGGATTACGCGATACGATATaa                           >  1:224096/1‑140 (MQ=255)

                                                                                           gAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGGAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATTCTCAACAGCAAACCGTCGTAACGGATTACGCGATACGATATAACATc                       >  2:276237/1‑140 (MQ=255)

                                                                                                     aTTAGCCAATATCTGACGAAAATCGGTTGGAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATTCTCAACAGCAAACCGTCGTAACGGATTACGCGATACGATATAACATCTGGAACTtta             <  1:276237/140‑1 (MQ=255)

                                                                                                      ttAGCCAATATCTGACGAAAATCGGTTGGAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATTCTCAACAGCAAACCGTCGTAACGGATTACGCGATACGATATAACATCTGGAACTttat            <  2:224096/140‑1 (MQ=255)

                                                                                                      ttAGCCAATATCTGACGAAAATCGGTTGGAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATTCTCAACAGCAAACCGTCGTAACGGATTACGCGATACGATATAACATCTGGAACTttat            <  1:528881/140‑1 (MQ=255)

                                                                                                          ccAATATCTGACGAAAATCGGTTGGAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATTCTCAACAGCAAACCGTCGTAACGGATTACGCGATACGATATAACATCTGGAACTttattatt        <  1:249348/140‑1 (MQ=255)

                                                                                                                tCTGACGAAAATCGGTTAGAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATTCTCAACAGCAAACCGTCGTAACGGATTACGCGATACGATATAACATCTGGAACTTTATTATTACAACt  <  1:517924/140‑1 (MQ=255)

GTGATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTGGCATAATGGGGAGTTGTCAACTATTGAAATGAAAAGTAAAACAATTCTCAACAGCAAACCGTCGTAACGGATTACGCGATACGATATAACATCTGGAACTTTATTATTACAACT  >  NC_000913/2552092‑2552343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A