Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A39 F60 I1 R1	19	46.8	1599832	99.2%	1587033	138.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,126,234	T→A	T97S (ACC→TCC)	glcE ←	glycolate dehydrogenase, putative FAD‑binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,126,234	0	T	A	100.0%	90.4 / NA	27	T97S (ACC→TCC)	glcE	glycolate dehydrogenase, putative FAD‑binding subunit
Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (10/17); total (10/17)

CATCACTTCGCCACCAAAACGCAGATGTTTTCCAGCGCCGGTAATGATGCGCGTGCCGAGGACAAAATCGCGGACCGAACCGCTCCACGGGCGACGCGGCCCCGCCAGCCCGCAGGCGACCATCCCGCCCCAGGTGGCTTCTTCACCATAATGCGGCGGCTCACAGGGGAGCATTTGCCCCGCGCTTTCCAGCGCCGCTTCAATTGTCACCAGCGGCGTTCCGACACGCGCGGTTATCACCAGCTCGGTCGGGTCGTAATTAACA  >  NC_000913/3126100‑3126364

catcaCTTCGCCACCAAAACGCAGATGTTTTCCAGCGCCGGTAATGATGCGCGTGCCGAGGACAAAATCGCGGACCGAACCGCTCCACGGGCGACGCGGCCCCGCCAGCCCGCAGGCGACCATCCCGCCCCAGGAGGctt                                                                                                                               <  1:297225/140‑1 (MQ=255)

   caCTTCGCCACCAAAACGCAGATGTTTTCCAGCGCCGGTAATGATGCGCGTGCCGAGGACAAAATCGCGGACCGAACCGCTCCACGGGCGACGCGGCCCCGCCAGCCCGCAGGCGACCATCCCGCCCCAGGAGGcttctt                                                                                                                            <  1:80448/140‑1 (MQ=255)

               aaaaCGCAGATGTTTTCCAGCGCCGGTAATGATGCGCGTGCCGAGGACAAAATCGCGGACCGAACCGCTCCACGGGCGACGCGGCCCCGCCAGCCCGCAGGCGACCATCCCGCCCCAGGAGGCTTCTTCACCATAATg                                                                                                                  <  1:48858/138‑1 (MQ=255)

               aaaaCGCAGATGTTTTCCAGCGCCGGTAATGATGCGCGTGCCGAGGACAAAATCGCGGACCGAACCGCTCCACGGGCGACGCGGCCCCGCCAGCCCGCAGGCGACCATCCCGCCCCAGGAGGCTTCTTCACCATAATg                                                                                                                  >  2:48858/1‑138 (MQ=255)

                aaaCGCAGATGTTTTCCAGCGCCGGTAATGATGCGCGTGCCGAGGACAAAATCGCGGACCGAACCGCTCCACGGGCGACGCGGCCCCGCCAGCCCGCAGGCGACCATCCCGCCCCAGGAGGCTTCTTCACCATAATgcgg                                                                                                               <  1:47797/140‑1 (MQ=255)

                         tGTTTTCCAGCGCCGGTAATGATGCGCGTGCCGAGGACAAAATCGCGGACCGAACCGCTCCACGGGCGACGCGGCCCCGCCAGCCCGCAGGCGACCATCCCGCCCCAGGAGGCTTCTTCACCATAATGCGGCGGCTcaca                                                                                                      <  2:15361/140‑1 (MQ=255)

                             ttCCAGCGCCGGTAATGATGCGCGTGCCGAGGACAAAATCGCGGACCGAACCGCTCCACGGGCGACGCGGCCCCGCCAGCCCGCAGGCGACCATCCCGCCCCAGGAGGCTTCTTCACCATAATGCGGCGGCTCACAgggg                                                                                                  <  2:2045/140‑1 (MQ=255)

                               ccAGCGCCGGTAATGATGCGCGTGCCGAGGACAAAATCGCGGACCGAACCGCTCCACGGGCGACGCGGCCCCGCCAGCCCGCAGGCGACCATCCCGCCCCAGGAGGCTTCTTCACCATAATgcgg                                                                                                               <  1:430986/125‑1 (MQ=255)

                               ccAGCGCCGGTAATGATGCGCGTGCCGAGGACAAAATCGCGGACCGAACCGCTCCACGGGCGACGCGGCCCCGCCAGCCCGCAGGCGACCATCCCGCCCCAGGAGGCTTCTTCACCATAATgcgg                                                                                                               >  2:430986/1‑125 (MQ=255)

                                  gcgcCGGTAATGATGCGCGTGCCGAGGACAAAATCGCGGACCGAACCGCTCCACGGGCGACGCGGCCCCGCCAGCCCGCAGGCGACCATCCCGCCCCAGGAGGCTTCTTCACCATAATGCGGCGGCTCACAGGGGAGCAt                                                                                             >  2:238591/1‑140 (MQ=255)

                                                 cgcgTGCCGAGGACAAAATCGCGGACCGAACCGCTCCACGGGCGACGCGGCCCCGCCAGCCCGCAGGCGACCATCCCGCCCCCGGAGGCTTCTTCACCATAATGCGGCGGCTCACAGGGGAGCATTTGCCCCGCGCTTTc                                                                              >  2:304499/1‑140 (MQ=255)

                                                  gcgTGCCGAGGACAAAATCGCGGACCGAACCGCTCCACGGGCGACGCGGCCCCGCCAGCCCGCAGGCGACCATCCCGCCCCAGGAGGCTTCTTCACCATAATGCGGCGGCTCACAGGGGAGCATTTGCCCCGCGCTTTcc                                                                             >  1:134328/1‑140 (MQ=255)

                                                     tGCCGAGGACAAAATCGCGGACCGAACCGCTCCACGGGCGACGCGGCCCCGCCAGCCCGCAGGCGACCATCCCGCCCCAGGAGGCTTCTTCACCATAATGCGGCGGCTCACAGGGGAGCATTTGCCCcgcg                                                                                   <  2:719176/131‑1 (MQ=255)

                                                     tGCCGAGGACAAAATCGCGGACCGAACCGCTCCACGGGCGACGCGGCCCCGCCAGCCCGCAGGCGACCATCCCGCCCCAGGAGGCTTCTTCACCATAATGCGGCGGCTCACAGGGGAGCATTTGCCCcgcg                                                                                   >  1:719176/1‑131 (MQ=255)

                                                       ccGAGGACAAAATCGCGGCCCGAACCGCTCCACGGGCGACGCGGCCCCGCCAGCCCGCAGGCGACCATCCCGCCCCAGGAGGCTTCTTCACCATAATGCGGCGGCTCACAGGGGAGCATTTGCCCCGCGCTTTCCAgcgc                                                                        <  1:796188/140‑1 (MQ=255)

                                                                                        gggCGACGCGGCCCCGCCAGCCCGCAGGCGACCATCCCGCCCCAGGAGGCTTCTTCACCATAATGCGGCGGCTCACAGGGGAGCATTTGCCCCGCGCTTTCCAGCGCCGCTTCAATTGTCACCAGCGGCGTTCCGACAcg                                       >  1:778115/1‑140 (MQ=255)

                                                                                          gCGACGCGGCCCCGCCAGCCCGCAGGCGACCATCCCGCCCCAGGAGGCTTCTTCACCATAATGCGGCGGCTCACAGGGGAGCATTTGCCCCGCGCTTTCCAGCGCCGCTTCAATTGTCACCAGCGGCGTTCCGACAcgcg                                     <  2:134328/140‑1 (MQ=255)

                                                                                            gACGCGGCCCCGCCAGCCCGCAGGCGACCATCCCGCCCCAGGAGGCTTCTTCACCATAATGCGGCGGCTCACAGGGGAGCATTTGCCCCGCGCTTTCCAGCGCCGCTTCAATTGTCACCAGCGGCGTTCCGACAcgcgcg                                   <  2:758424/140‑1 (MQ=255)

                                                                                             aCGCGGCCCCGCCAGCCCGCAGGCGACCATCCCGCCCCAGGAGGCTTCTTCACCATAATGCGGCGGCTCACAGGGGAGCATTTGCCCCGCGCTTTCCAGCGCCGCTTCAATTGTCACCAgcg                                                    <  2:303556/122‑1 (MQ=255)

                                                                                             aCGCGGCCCCGCCAGCCCGCAGGCGACCATCCCGCCCCAGGAGGCTTCTTCACCATAATGCGGCGGCTCACAGGGGAGCATTTGCCCCGCGCTTTCCAGCGCCGCTTCAATTGTCACCAgcg                                                    >  1:303556/1‑122 (MQ=255)

                                                                                               gcgGCCCCGCCAGCCCGCAGGCGACCATCCCGCCCCAGGAGGCTTCTTCACCATAATGCGGCGGCTCACAGGGGAGCATTTGCCCCGCGCTTTCCAGCGCCGCTTCAATTGTCACCAGCGGCGTTCCGACACGCGCGGtt                                <  2:778115/140‑1 (MQ=255)

                                                                                                        ccAGCCCGCAGGCGACCATCCCGCCCCAGGAGGCTTCTTCACCATAATGCGGCGGCTCACAGGGGAGCATTTGCCCCGCGCTTTCCAGCGCCGCTTCAATTGTCACCAGCGGCGTTCCGACACGCGCGGTTATCACCAGc                       >  2:634878/1‑140 (MQ=255)

                                                                                                                 aGGCGACCATCCCGCCCCAGGAGGCTTCTTCACCATAATGCGGCGGCTCACAGGGGAGCATTTGCCCCGCGCTTTCCAGCGCCGCTTCAATTGTCACCAGCGGCGTTCCGACACGCGCGGTTATCACCAGCTCggtcggg              <  1:634878/140‑1 (MQ=255)

                                                                                                                 aGGCGACCATCCCGCCCCAGGAGGCTTCTTCACCATAATGCGGCGGCTCACAGGGGAGCATTTGCCCCGCGCTTTCCAGCGCCGCTTCAATTGTCACCAGCGGCGTTCCGACACGCGCGGTTATCACCAGCTCggtcggg              <  1:546069/140‑1 (MQ=255)

                                                                                                                  ggCGACCATCCCGCCCCAGGAGGCTTCTTCACCATAATGCGGCGGCTCACAGGGGAGCATTTGCCCCGCGCTTTCCAGCGCCGCTTCAATTGTCACCAGCGGCGTTCCGACACGCGCGGTTATCACCAGCTCggtcgggt             >  2:80071/1‑140 (MQ=255)

                                                                                                                        caacccGCCCCAGGAGGCTTCTTCCCCATAATGCGGCGGCTCACAGGGGAGCATTTGCCCCGCGCTTTCCAGCGAAGCTTCAATTGTCACCAGCGGCGTTCCGACACGCGCGGTTATCACCAGCTCGGTCGGGTCGTAAt       <  1:389558/137‑1 (MQ=255)

                                                                                                                             cGCCCCAGGAGGCTTCTTCACCATAATGCGGCGGCTCACAGGGGAGCATTTGCCCCGCGCTTTCCAGCGCCGCTTCAATTGTCACCAGCGGCGTTCCGACACGCGCGGTTATCACCAGCTCGGTCGGGTCGTAATTAACa  <  1:80071/140‑1 (MQ=255)

CATCACTTCGCCACCAAAACGCAGATGTTTTCCAGCGCCGGTAATGATGCGCGTGCCGAGGACAAAATCGCGGACCGAACCGCTCCACGGGCGACGCGGCCCCGCCAGCCCGCAGGCGACCATCCCGCCCCAGGTGGCTTCTTCACCATAATGCGGCGGCTCACAGGGGAGCATTTGCCCCGCGCTTTCCAGCGCCGCTTCAATTGTCACCAGCGGCGTTCCGACACGCGCGGTTATCACCAGCTCGGTCGGGTCGTAATTAACA  >  NC_000913/3126100‑3126364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A