Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A24 F60 I1 R1	19	84.9	2879288	99.1%	2853374	137.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,140,928	A→G	I120T (ATC→ACC)	hybD ←	putative hydrogenase 2 maturation protease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,140,928	0	A	G	100.0%	175.8 / NA	47	I120T (ATC→ACC)	hybD	putative hydrogenase 2 maturation protease
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (23/24); total (23/24)

TCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCGGCCAGGCCAAGCTGATGCGGAGAGATTTTGTTGGTAAACAACGCCGGAACTTCTTCATCCCGCAGGAT  >  NC_000913/3140792‑3141061

tCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGGTCa                                                                                                                                    >  1:535334/1‑140 (MQ=255)

  aGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGGTCACg                                                                                                                                  <  2:535334/140‑1 (MQ=255)

   gTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGGTCACGc                                                                                                                                 >  2:579140/1‑140 (MQ=255)

              ccTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGGTCACGCCGACCAGGGTc                                                                                                                      >  2:1023106/1‑140 (MQ=255)

                    gTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGGTCACGCCGACCAGGGTCAGCttt                                                                                                                >  2:657170/1‑140 (MQ=255)

                          tGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGGTCACGCCGACCAGGGTCAGCTTTTTCGGa                                                                                                          <  1:1099059/140‑1 (MQ=255)

                            gCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGGTCACGCCGACCAGGGTCAGCTTTTTCGGaaa                                                                                                        >  1:380401/1‑140 (MQ=255)

                                   cGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGGTCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCg                                                                                                 >  1:1147478/1‑140 (MQ=255)

                                      cAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGGTCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTg                                                                                              <  2:1064429/140‑1 (MQ=255)

                                      cAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGGTCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTg                                                                                              >  1:332368/1‑140 (MQ=255)

                                      cAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGGTCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTg                                                                                              <  2:380401/140‑1 (MQ=255)

                                                cagcgcagcCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGGTCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAggg                                                                                    <  1:370589/140‑1 (MQ=255)

                                                        ccAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGGTCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAg                                                                            >  2:457289/1‑140 (MQ=255)

                                                             aCCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGGTCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGAc                                                                               >  2:1382264/1‑132 (MQ=255)

                                                             aCCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGGTCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGAc                                                                               <  1:1382264/132‑1 (MQ=255)

                                                                tGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGGTCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCggc                                                                    <  1:361886/140‑1 (MQ=255)

                                                                tGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGGTCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCggc                                                                    <  1:454385/140‑1 (MQ=255)

                                                                 gCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGGTCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCggcc                                                                   <  2:332368/140‑1 (MQ=255)

                                                                  cTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGGTCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCggcca                                                                  >  1:367361/1‑140 (MQ=255)

                                                                      aGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGGTCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCggccaggcc                                                              <  1:568768/140‑1 (MQ=255)

                                                                             gTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGGTCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCGGCCAGGCCAAGCTGa                                                       <  1:457289/140‑1 (MQ=255)

                                                                                       tGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGGTCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCGGCCAGGCCAAGCTGATGCGgagaga                                             >  1:1034292/1‑140 (MQ=255)

                                                                                         cTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGGTCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCGGCCAGGCCAAGCTGATGCg                                                   <  1:521428/132‑1 (MQ=255)

                                                                                         cTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGGTCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCGGCCAGGCCAAGCTGATGCg                                                   >  2:521428/1‑132 (MQ=255)

                                                                                           tCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGGTCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCGGCCAGGCCAAGCTGATGCGGAGAGAtttt                                         <  1:657170/140‑1 (MQ=255)

                                                                                             aaCCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGGTCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCGGCCAGGCCAAGCTGATGCg                                                   <  2:743651/128‑1 (MQ=255)

                                                                                             aaCCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGGTCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCGGCCAGGCCAAGCTGATGCGGAGAGATTttgt                                       <  1:902342/140‑1 (MQ=255)

                                                                                             aaCCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGGTCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAAACGTCGGCCAGGCCAAGCTGATGCg                                                   >  1:743651/1‑128 (MQ=255)

                                                                                                 gTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGGTCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCGGCCAGGCCAAGCTGATGCGGAGAGATTTTGTTGGt                                   >  1:446980/1‑140 (MQ=255)

                                                                                                  tCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGGTCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCGGCCAGGCCAAGCTGATGCGGAGAGATTTTGTTGGTa                                  >  2:234629/1‑140 (MQ=255)

                                                                                                    ggCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGGTCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCGGCCAGGCCAAGCTGATGCGGAGAGATTTTGTTGGTaaa                                >  2:536105/1‑140 (MQ=255)

                                                                                                         tAAGCCGATGTGTGGCTCCAGCGATTCCGGGGTCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCGGCCAGGCCAAGCTGATGCGGAGAGATTTTGTTGGTAAACAACg                           >  1:318649/1‑140 (MQ=255)

                                                                                                           aGCCGATGTGTGGCTCCAGCGATTCCGGGGTCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCGGCCAGGCCAAGCTGATGCGGAGAGATTTTGTTGGTAAACAACGcc                         <  2:1034292/140‑1 (MQ=255)

                                                                                                               gATGTGTGGCTCCAGCGATTCCGGGGTCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCGGCCAGGCCAAGCTGATGCGGAGAGATTTTGTTGGTAAACAACGCCGGaa                     <  2:367361/140‑1 (MQ=255)

                                                                                                                      ggCTCCAGCGATTCCGGGGTCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCGGCCAGGCCAAGCTGATGCGGAGAGATTTTGTTGGTAAACAACGCCGGAACttcttc              <  1:234629/140‑1 (MQ=255)

                                                                                                                       gCTCCAGCGATTCCGGGGTCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCGGCCAGGCCAAGCTGATGCGGAGAGATTTTGTTGGTAAACAACGCCGGAACTTCTTCa             <  2:318649/140‑1 (MQ=255)

                                                                                                                       gCTCCAGCGATTCCGGGGTCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCGGCCAGGCCAAGCTGATGCGGAGAGATTTTGTTGGTAAACAACGCCGGAACTTCTTCa             <  1:536105/140‑1 (MQ=255)

                                                                                                                        cTCCAGCGATTCCGGGGTCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCGGCCAGGCCAAGCTGATGCGGAGAGATTTTGTTGGTAAACAACGCCGGATCTTCTTCAt            <  2:600383/140‑1 (MQ=255)

                                                                                                                           cAGCGATTCCGGGGTCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCGGCCAGGCCAAGCTGATGCGGAGAGATTTTGTTGGTAAACAACGCCGGAACTTCTTCATccc         <  2:787130/140‑1 (MQ=255)

                                                                                                                           cAGCGATTCCGGGGTCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCGGCCAGGCCAAGCTGATGCGGAGAGATTTTGTTGGTAAACAACGCCGGAACTTCTTCATccc         <  2:446980/140‑1 (MQ=255)

                                                                                                                            aGCGATTCCGGGGTCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCGGCCAGGCCAAGCTGATGCGGAGAGATTTTGTTGGTAAACAACGCCGGAACTTCTTCATCCCg        >  1:1128775/1‑140 (MQ=255)

                                                                                                                             gtgATTCCGGGGTCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCGGCCAGGCCAAGCTGATGCGGAGAGATTTTGTTGGTAAACAACGCCGGAACTTCTTCATCCCGc       >  2:774838/3‑140 (MQ=255)

                                                                                                                             gCGATTCCGGGGTCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCGGCCAGGCCAAGCTGATGCGGAGAGATTTTGTTGGTAAACAACGCCGGAACTTCTTCATCCCGc       >  1:165821/1‑140 (MQ=255)

                                                                                                                                 ttCCGGGGTCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCGGCCAGGCCAAGCTGATGCGGAGAGATTTTGTTGGTAAACAACGCCGGAACTTCTTCATCCCGCAGGa   >  2:1164379/1‑140 (MQ=255)

                                                                                                                                 ttCCGGGGTCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCGGCCAGGCCAAGCTGATGCGGAGAGATTTTGTTGGTAAACAACGCCGGAACTTCTTCATCCCGCAGGa   >  2:114696/1‑140 (MQ=255)

                                                                                                                                  tCCGGGGTCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCGGCCAGGCCAAGCTGATGCGGAGAGATTTTGTTGGTAAACAACGCCGGAACTTCTTCATCCCGCAGGat  <  1:319964/140‑1 (MQ=255)

                                                                                                                                  tCCGGGGTCACGCCGACCAGGGTCAGCTTTTTCAGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCGGCCAGGCCAAGCTGATGCGGAGAGATTTTGTTGGTAAACAACGCCGGAACTTCTTCATCCCGCAGGat  >  2:379893/1‑140 (MQ=255)

TCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCGGCCAGGCCAAGCTGATGCGGAGAGATTTTGTTGGTAAACAACGCCGGAACTTCTTCATCCCGCAGGAT  >  NC_000913/3140792‑3141061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A